目的 :观察褪黑素和电针对创伤大鼠脾脏淋巴细胞转化功能、ConA诱导的IL 2活性及脾淋巴细胞、血浆、下丘脑及垂体ACTH含量的影响。方法 :MTT法检测淋巴细胞转化功能、IL 2活性

放射免疫分析法检测各组织ACTH的含量。结果 :腹腔注射褪黑素能有效逆转手术创伤大鼠的淋巴细胞转化功能

低水平IL 2诱生活性得到恢复

同时创伤后升高的脾淋巴细胞、血浆、下丘脑ACTH水平下降

而垂体内降低的ACTH水平回升。电针刺激“足三里”、“阑尾”穴也能明显改善创伤应激诱导的免疫抑制

并降低脾淋巴细胞、血浆、下丘脑ACTH含量

增加垂体ACTH含量。褪黑素与电针合用可进一步调节被抑制的免疫功能

且与单独褪黑素或电针相比有显著差异。结论 :褪黑素和 /或电针能恢复创伤大鼠降低的淋巴细胞转化功能、IL 2诱生活性

并调节机体ACTH水平Objective: To observe the effect of melatonin (MT) and electroacupuncture (EA) on the spleen lymphocyte proliferation

concanavaline (Con) A induced IL 2 activity and ACTH content in the spleen lymphocyte supernate

plasma

hypothalamus and the pituitary of traumatic rats. Methods: SD rats with body weight of 200±20 g were randomly divided into normal control group

traumatic injury (TI) +MT solvent control group

TI+EA group

TI+MT group and TI+MT+EA group

with 6 cases in each group. Following anesthesia with pentobarbital sodium (0.4%

2 mL)

a longitudinal incision was made along the back and the abdominal midline to mimic clinical visceral exploring surgical operation for establishing traumatic injury model. EA (2 Hz

1 mA) was applied to "Zusanli"(ST 36) and "Lanwei"(Extra 37) for 60 min before intraperitoneal (i.p.) injection of MT (5%

5 mg/kg) that was given at 4～5 o'clock of the operation day. MTT colorimetric assay was used to detect lymphocyte proliferation rate and Con A induced IL 2 activity of the spleen lymphocyte supernate

and radioimmunoassay was used to detect ACTH contents in the spleen lymphocytes

plasma

hypothalamus and the pituitary. Results: Administration of MT and EA were able to revise TI generated depression of the lymphocyte proliferation rate and IL 2 activity ( P <0.05) compared with TI+MT solvent control group. In comparison with MT+TI and EA+TI groups

both the lymphocyte proliferation rate and IL 2 activity were raised further in TI+MT+EA group ( P <0.05). Intraperitoneal administration of MT and EA could considerably lower traumatic injury induced increase of ACTH levels in the spleen lymphocytes ( P <0.001)

plasma ( P <0.05) and hypothalamus ( P <0.05)

and significantly inhibit TI induced decrease of ACTH in the pituitary ( P <0.05

vs TI group respectively). When MT and EA used together

TI induced increase of ACTH levels in the spleen lymphocytes

plasma and the hypothalamus was lowered further ( P <0.05

vs TI+EA or MT group)

while the decreased ACTH content in the pituitary was raised further ( P <0.05

vs TI+EA or MT group). Conclusion: MT and/or EA could recover TI induced changes of lymphocyte proliferation and Con A generated changes of IL 2 activity

modulate ACTH levels in the spleen lymphocytes plasma

hypothalamus and the pituitary in traumatic rats.