目的 :观察不同剂量曲马多对关节炎大鼠热痛敏的镇痛作用及曲马多对针刺镇痛的影响。方法 :在完全弗氏佐剂关节炎大鼠模型上采用辐射热缩腿反射的方法

以痛敏分数作为观察指标

分别腹腔注射 (i.p .) 5mg·kg- 1 ·d- 1 、1 0mg·kg- 1 ·d- 1 、2 0mg·kg- 1 ·d- 1 曲马多和 1 0mL·kg- 1 ·d- 1 生理盐水 1 0天

记录每次给药 2 4hr后痛敏分数变化。继而选择 1 0mg·kg- 1 ·d- 1 曲马多与电针合用

观察单独应用 1 0mg·kg- 1 ·d- 1 曲马多、单独应用电针、曲马多与电针合用及1 0mL·kg- 1 ·d- 1 生理盐水四组 (均为 1 0天 )

每次给药或 /和电针 2 4hr后痛敏分数变化。结果 :i.p .5mg·kg- 1 ·d- 1 曲马多连续应用 1 0天时

大鼠痛敏分数无明显变化 ;i.p.1 0mg·kg- 1 ·d- 1 曲马多 9天时大鼠痛敏分数显著提高 ;i.p .2 0mg·kg- 1 ·d- 1 曲马多 6天时大鼠的痛敏分数显著提高 ;单独应用电针 8天时大鼠痛敏分数显著提高 ;i.p.1 0mg·kg- 1 ·d- 1 曲马多与电针合用 4天时可明显提高大鼠痛敏分数。结论 :长期应用曲马多对慢性炎症痛大鼠具有镇痛作用

呈剂量相关性 ;曲马多与电针合用可加强电针的镇痛作用

并降低曲马多使用剂量。提示曲马多与电针合用是一种治疗慢性痛的有效方法Objective:To investigate the antinociceptive effects of chronically using different dose of tramadol and the effect of tramadol on acupuncture analgesia in arthritis rats. Methods: SD rat arthritis model was established by injection of complete Freund's adjuvant 0.05 mL into the left ankle joint. The paw withdrawal latency (PWA)was detected with a radiant heat algesimeter. The difference between PWA of the inflammatory side and the healthy side was used as the hyperalgesia score. By using paw withdrawal latency to radiant heat stimulation and hyperalgesia score as the endpoint of nociceptive test

5 mg·kg -1 ·d -1

10 mg·kg -1 ·d -1 and 20 mg·kg -1 ·d -1 tramadol and 1 mL·kg -1 ·d -1 0.9% normal saline (NS) were respectively administrated intraperitoneally

once everyday and continuously for ten days. The changes of hyperalgesia score were observed 24 hr after drug administration everyday following i.p. 10 mg ·kg -1 ·d -1 tramadol

electroacupuncture (EA)

EA combined with i.p.10 mg·kg -1 ·d -1 tramadol and i.p. 1 mL·kg -1 ·d -1 0.9% NS (4 groups)

once everyday for ten days. Results: I.p. 5 mg·kg -1 ·d -1 tramadol for ten days did not show any effect ( P > 0.05) . I.p.10 mg·kg -1 ·d -1 tramadol for nine days

i.p. 20 mg·kg -1 ·d -1 for six days and using EA for eight days elevated hyperalgesia score significantly in comparison with NS group ( P <0.05～ 0.01) . Then i.p.10 mg·kg -1 ·d -1 tramadol combined with electroacupuncture for four days

hyperalgesia score was elevated significantly in comparison with NS group ( P <0.05～0.01) and with EA group ( P <0.05). Conclusion: The results show that tramadol has dose relative antinociceptive effect

and tramadol can enhance the analgesic effect of acupuncture under lower dose

suggesting that combination of acupuncture with tramadol is one of the effective treatment methods for chronic pain.