目的 :比较针刺不同穴组对全脑缺血再灌注大鼠外周血WBC(白细胞 )、血清TNF α(肿瘤坏死因子 α)、IL 6 (白细胞介素 6 )和脑组织自由基的影响。方法 :四动脉结扎法造成大鼠全脑缺血再灌注模型

分别针刺大鼠阳明经“曲池”、“足三里”穴 (针刺二组 )与针刺“曲池”、“足三里”合用督脉“后顶”穴 (针刺一组 )

测定外周血WBC、血清TNF α、IL 6值及脑组织LPO(过氧化脂质 )、GSH(谷胱甘肽 )含量。结果 :大鼠全脑缺血再灌注后

外周血WBC、血清TNF α、IL 6值及脑组织LPO含量上升

GSH含量下降

针刺二组可降低大鼠全脑缺血再灌注后升高的IL 6水平

降低脑组织LPO水平 (P <0 0 5)

针刺一组可降低全脑缺血再灌注后升高的WBC、TNF α和IL 6水平

降低脑组织LPO水平 (P <0 0 1 )

提高GSH含量。结论 :针刺“曲池”、“足三里”合用督脉“后顶”穴比单纯应用“曲池”、“足三里”穴对全脑缺血再灌注大鼠外周血WBC、血清TNF α、IL 6和脑组织自由基的影响更强

从而表明对全脑缺血再灌注大鼠脑组织具有更好的保护作用Objective: To observe the effects of electroacupuncture (EA) of different acupoint groups on blood WBC (white blood cells)

TNF (tumor necrosis factor)-α

IL (interleukin)-6 and cerebral free radical levels in cerebral ischemia-reperfusion rats.Methods:A total of 43 Wistar rats were randomly divided into normal control group (n=8)

Sham-operation group (n=8)

ischemia-reperfusion (model) group (n=9)

acupuncture (A)-I group (n=10) and A-II group (n=8). A four-arteries-ligation method was used to make cerebral ischemia-reperfusion model. In acupuncture-I group

"Quchi" (LI 11) and "Zusanli" (ST 36) were punctured and stimulated electrically (30 Hz

3～6 V

20 min) and "Houding" (GV 19) was punctured and stimulated by manipulating the acupuncture needle with uniform reinforcing-reducing method. In acupuncture-II group

only EA was applied to LI-11 and ST-36 with the same parameters. Blood samples were collected from the abdominal aorta to be processed into supernatant for measuring TNF-α

IL-6 and free radicals with radioimmunoassay. Cerebral tissues were taken after decapitation to be processed into supernate for detecting GSH and LPO contents with a spectrophotometer (colorimetric analysis). Peripheral blood WBC count was detected with routine method.Results:In comparison with normal control and sham-operation groups

blood WBC count

serum TNF-α and IL-6 and cerebral LPO (lipid peroxide) increased significantly (P< 0.01～0.001)

and GSH (glutathione) decreased significantly (P<0.001) in model group. After acupuncture treatment

compared with model group

WBC of A-I group

TNF-α

IL-6 and LPO of A-I group

and IL-6 of A-II group all declined considerably (P<0.05～0.01)

while GSH of A-I group increased significantly (P<0.05)

showing that acupuncture can apparently lower WBC

TNF-α

IL-6 and LPO and raise GSH content. Comparison between two acupuncture groups showed that only LPO of A-I group was significantly lower than that of A-II group (P<0.05). Conclusion:EA of "Quchi" (LI 11) and "Zusanli" (ST 36) combined with acupuncture of "Houding" (GV 19) is superior to simple EA of LI-11 and ST-36 in regulating blood WBC

serum TNF-α and IL-6

and cerebral tissue free radical levels in cerebral ischemia-reperfusion rats.