目的 :了解电针对面神经损伤后再生微环境中神经营养因子 (NGF)的影响。方法 :采用同龄成年健康的新西兰兔 50只

随机分为针刺组和对照组

均在 3 %的戊巴比妥钠静脉麻醉下

分离暴露面神经上颊支

在手术放大镜下切断神经

用硅胶管将两断端嵌入并缝合固定

形成再生室。针刺组于术后当天完全醒后开始接受电针治疗

每天 1次

对照组不作任何处理。术后3天、5天、7天、1 0天、1 4天分别处死 1 0只动物

针刺组与对照组各 5只。抽取再生室内液体

两组对照。结果 :在伤后 3～ 7天

针刺组和对照比较再生室内NGF浓度差别无显著性 (P >0 .0 5)

接受电针治疗 1 0天和 1 4天

针刺组再生室内NGF浓度比对照组高 (P <0 .0 1 )

伤后第 7天

针刺组和对照组再生室内NGF浓度均达到峰值

但对照组在伤后 2周开始降低

针刺组仍保持在较高的水平。结论 :电针对面神经损伤后再生微环境中神经生长因子 (NGF)的浓度有提高其水平和维持平稳水平的作用

这可能是电针刺激促进周围神经再生机理的一方面Objective: To observe the effect of electroacupuncture (EA) on NGF level in the injured facial nerve regeneration chamber in the rabbits. Methods: Facial nerve injury was induced by transectioning the upper buccal branch of the facial nerve about 5 mm in length that was then fixed in a 12 mm long silica gel tube (chamber). 50 facial nerve injury rabbits were randomized into control and EA groups

with 25 cases in each group. EA (18～20 Hz

1.5 V

30 min/time) was applied to "Yifeng" (TE 17)

"Dicang" (ST 4) to "Jiache" (ST 6) and "Hegu" (LI 4)

once daily

continuously for 3～14 days. EA and control groups were respectively divided into 3 d

5 d

7 d

10 d

14 d subgroups. The fluids samples accumulated in the chamber were collected at different postoperative intervals: 3 d

5 d

7 d

10 d

14 d for detecting NGF content using double antibody Sandwiched ELISA. Results: In 7 d control and EA subgroups

NGF levels peaked

and decreased in 10 d and 14 d control subgroups

suggesting that EA can maintain the higher secretion level. NGF contents of the 5 EA subgroups particularly 10 d and 14 d subgroups ( P <0.01) were all higher or significantly higher than those of the corresponding 5 control subgroups

indicating that EA treatment can potentiate the secretion of NGF of the injured facial nerve and the effect of EA peaked on the 10 th day. In 7 d control and EA subgroups

NGF levels peaked

and decreased in 10 d and 14 d control subgroups

suggesting that EA can maintain the higher secretion level. Conclusion: Electroacupuncture therapy can promote the secretion of NGF of the injured nerve which may contribute to the effect of acupuncture in promoting the regeneration of the injured nerve.