目的 :通过肿瘤坏死因子 (TNFα)基因表达

研究针灸治疗绝经后骨质疏松症的作用机理。方法 :8个月龄SD雌性大鼠 40只

双侧卵巢切除后

均分为模型、针刺、艾灸、西药四组。相同水、饲料喂养 3个月后

针刺、艾灸、西药组治疗 3个疗程。将 40只鼠断头取血

检测血清中骨钙素(BGP)、雌二醇 (E2 )含量 ;取双侧股骨测骨密度 ;无菌条件下取出大鼠右侧胫骨

分离骨髓细胞 ;利用对L92 9细胞的杀伤作用检测TNFαmRNA生物活性 ;利用RT PCR技术检测TNFαmRNA表达。结果 :针刺、艾灸组BGP、E2 较模型组有显著增高 (P <0 0 5) ;L92 9死亡率较模型组明显减少(P <0 0 0 1) ;RT PCR显示TNFαmRNA表达较模型组明显减少。结论 :针刺、艾灸治疗绝经后骨质疏松症是在基因转录后水平上进行的

即针灸治疗绝经后骨质疏松症是通过调控TNFαmRNA表达

从而控制TNFα蛋白合成来实现的Objective:To observe the effect of acupuncture on tumor necrosis factor (TNFα)expression in bone marrow of experimental osteoporosis rats for studying mechanisms of acupuncture in the treatment of postmenopausal osteoporosis. Methods:40 ovariectomized SD rats were evenly divided into control

acupuncture

moxibustion and medication groups. In acupuncture group

“Mingmen" (GV 4)

“Dazhui"(GV 14)

“Dazhu"(BL 11)

“Shenshu"(BL 23)

“Pishu"(BL 20) and “Zusanli" (ST 36) were punctured and stimulated with uniform reinforcing and reducing method (once every other day

36 times altogether). In moxibustion group

the abovementioned acupoints were used for moxibustion

2 moxa cones every acupoint. Animals of medication group were fed with Nilestriol (0.5 mL/100 g

once every week). The contents of serum ositeocalcin(BGP)and estradiol (E 2)contents were measured with radioimmunoassay. Bone mineral density(BMD)of bilateral femora

tumor necrosis factor (TNF)α bioactivity (L929 cell death rates) were measured and TNFα expression of the bone marrow (tibia) tissue cells was measured with RT PCR method.Results: Contents of BGP and E 2 of acupuncture and moxibustion and E 2 of medication groups were obviously higher than those of control group (P<0.05

0.01). BGP level of medication group was also significantly lower than those of acupuncture and moxibustion groups (P<0.01). No significant differences were found among acupuncture

moxibustion and medication groups in E 2 levels

and between acupuncture and moxibustion groups in BGP levels (P>0.05). The BMD values of the 3 treatment groups were all significantly higher than those of model group (P<0.05); while TNFα bioactivity of the 3 treatment groups were considerably lower than that of control group (P<0.001). No significant differences were found in BMD and L929 death rates among the 3 treatment groups (P>0.05). The situations of TNFα mRNA expression presented a similar tendency to those of L929 death rate. Results suggest that acupuncture and moxibustion induced upregulation of BGP and E 2 and downregulation of TNFα mRNA expression and TNFα bioactivity may contribute to their effect in resisting osteoporosis. Conclusion:Acupuncture and moxibustion can relieve the decrease of E 2 and BGP levels

and downregulate TNFα mRNA expression and the bioactivity of TNFα in ovariectomized rats.