目的 :观察电针对脑缺血大鼠谷氨酸、门冬氨酸、甘氨酸及氨基丁酸含量的影响。方法 :利用大鼠大脑中动脉阻塞再灌流模型

采用高效液相 色谱法测定四种氨基酸含量。结果 :大鼠局脑缺血 3 0min

再灌流 4hr后

Glu、ASP含量明显升高 (P <0 0 1

P <0 0 5)

GABA和Gly含量无明显变化 ;而经电针治疗组Glu、ASP含量显著下降 (P <0 0 1 )

GABA含量升高 (P <0 0 5)

Gly含量无明显变化。结论 :电针可抑制兴奋性氨基酸的过量堆积

EAA的减少与GABA的增加相关Objective: To study the effects of electroacupuncture (EA) on glutamate(Glu)

aspartate(Asp)

Glycine (Gly) and γ-Aminobuyric acid(GABA) in the brain tissue in rats with cerebral ischemia-reperfusion (CIR).Methods: Twenty-four Wistar rats were randomly and evenly divided into control group

CIR group and CIR+EA group. CIR model was established by occlusion of the middle cerebral artery for 30 min and removing the occlusion for 4 hours. "Suliao" (GV 25) and "Baihui" (GV 20) were punctured and stimulated with an EA therapeutic apparatus (dense-sparse waves

5/ 45 Hz

0.3 mA) for 30 min. High pressure liquid chromatography (HPLC)-fluorescence assay was used to detect contents of Glu

Asp

Gly and GABA in rat brain tissue before and after EA. Results: Four and half hours after CIR

Glu and Asp contents of the brain tissue increased significantly(P< 0.01

P<0.05)

Gly and GABA levels had no obvious changes. After EA treatment

cerebral Glu and Asp levels reduced considerably (P<0.01)

GABA level increased strikingly (P<0.05)

and Gly content had no evident change. Conclusion: EA can reduce excessive accumulation of excitatory amino acids (Glu and Asp) in the brain that may be related to EA-induced increase of GABA. The regulative action of EA on excitatory and inhibitory amino acid contents in the brain may contribute to EA's neuroprotective effect on ischemic cerebral tissue.