目的 :观察电针对大鼠蓝斑 (locusceruleus

LC)内一氧化氮合酶 (NOS)阳性神经元表达的影响。方法 :采用还原型尼克酰胺腺嘌呤二核苷酸脱氢酶 (NADPH d)法结合计算机图像分析技术

观察电针大鼠一侧“足三里”穴对LC内NOS表达的影响。结果 :电针大鼠一侧“足三里”穴后

同侧LC内NOS阳性神经元的数量增多 (P <0 .0 5)

染色加深

细胞平均灰度值明显降低(P <0 .0 1) ;非电针侧较正常组无明显改变 (P >0 .0 5)。结论 :电针对大鼠同侧LC内NOS阳性神经元的表达有上调作用Objective: To observe the effect of electroacupuncture (EA) on nitric oxide synthase (NOS) expression of neurons in locus ceruleus (LC) in rats. Methods: 16 SD rats were evenly randomized into control group and EA group. EA (3 V

2 Hz

wave duration 1 ms) was applied to unilateral “Zusanli"(ST 36) for 30 min. NOS expression was assayed with nicotinamide adenosine dinucleotide phosphate diaphorase (NADPH d) method and measured by using computer image analysis system to detect the optical density value of the stained tissue slices

and morphological changes of NOS positive neurons were observed under light microscope. Results: In animals of control group

most NOS immunoreaction (IR) positive neurons of LC presented moderate density of staining and fewer showed dense and mild staining

while in animals of EA group

NOS IR positive neurons of LC on EA side increased significantly in number in comparison with those of the contralateral side of EA. In addition

on EA side

densely stained neurons in LC increased apparently in number

cellular average optical density value lowered strikingly (P<0.01). No significant differences were found between EA group (non EA side) and control group in IR positive neuron number and the average optical density value (P>0.05). Conclusion: Electroacupuncture of “Zusanli"(ST 36) can upregulate NOS expression of neurons in the ipsilateral LC.