目的 :探讨早期针刺治疗抗脑缺血再灌注炎性损伤的机制。方法 :采用大脑中动脉线栓法制备MCAO缺血再灌注模型

应用原位杂交的方法观察脑缺血再灌注及电针对大鼠大脑皮质、纹状体IL 1 βmRNA、TNF αmRNA表达的影响。结果 :脑缺血再灌注后

缺血侧皮层及纹状体IL 1 βmRNA、TNF αmRNA免疫阳性细胞表达增高 (模型组与正常组、假手术组比较P <0 0 1 ) ;电针可显著降低缺血侧皮层及纹状体IL 1 βmRNA、TNF αmRNA免疫阳性细胞表达 (电针组与模型组比较P <0 0 1 )。结论 :早期针刺治疗可能通过下调缺血脑区IL 1 βmRNA、TNF αmRNA免疫阳性细胞而防治脑缺血再灌注炎性损伤Objective: To explore into mechanisms of early treatment of cerebral ischemia-reperfusion (IR) by acupuncture in resisting inflammation injury in rats. Methods: Forty Wistar rats were randomly and evenly assigned to control

sham-operation

IR-6hr

IR-12hr

IR-24hr

IR-6hr-EA

IR-12hr-EA and IR-24hr-EA groups. Cerebral IR model was established by occlusion of the middle cerebral artery for 30 min and then removal of the occlusion for 6

12 and 24 hr respectively. Electroacupuncture (EA

4～16 Hz

1～3 V

30 min) was delivered via acupuncture needles inserted into "Shuigou"(GV 26) and "Baihui"(GV 20). The expression of IL-1β mRNA?TNF-α mRNA of neurons of the cerebral tissue was displayed using in situ hybridization technique after IR and assayed with a spectrometer system. Results: When compared with control group

the expression of IL-1β mRNA and TNF-α mRNA in the cerebral cortex and striate body on the ischemic side in IR-6hr

IR-12hr and IR-24hr groups increased significantly (P<0.01); while compared with IR-6hr

IR-12hr and IR-24hr groups separately

the expression of IL-1β mRNA and TNF-α mRNA of IR-6hr-EA

IR-12hr-EA and IR-24hr-EA groups was down-regulated considerably (P<0.01) despite being still higher than those of control group evidently. It suggested that EA could suppress the IR-induced over-expression of IL-1β mRNA and TNF-α mRNA of neurons in the brain tissues. Conclusion:The protective effect of acupuncture on cerebral neurons under acute cerebral infarction condition is possible through its downregulative action on IL-1β mRNA and TNF-α mRNA expression.