目的 :观察电针“内关”对内毒素休克时肝功能的保护作用和肝组织一氧化氮合酶(NOS)表达的影响。方法 :静脉注射内毒素 (LPS) 1.5mg/kg、腹腔注射D 氨基半乳糖 (D GalN)10 0mg/kg造成内毒素休克模型

用电针“内关”和氨基胍 (AG)作对照干预处理

取血清检测肝功四项

用免疫组化方法检测诱生型一氧化氮合酶 (iNOS)、内皮型一氧化氮合酶 (eNOS)在肝组织内的表达。结果 :电针“内关”可以使肝功四项中ALT、AST、LDH值显著下降

使肝内iNOS表达减弱、eNOS表达增强

肝损害减轻。结论 :电针“内关”可以减轻内毒素休克造成的肝损害

这种保护作用可能是通过调节肝组织NOS表达而产生的Objective: To observe the effect of electroacupuncture (EA) of “Neiguan" (PC 6) on acute liver injury and liver NOS expression in endotoxin shock rats. Methods: 50 SD rats were randomly and evenly divided into control

model

Aminoguanidine Hemisulfate (AG)

EA and EA+AG groups. Endotoxin shock model was induced by intravenous injection of Lipopolysaccharide (LPS

1.5 mg/kg) and intraperitoneal injection of D galactosamine (D GalN

100 mg/kg). The expression of iNOS and eNOS was measured by using immunohistochemical method. Serum alanine aminotransferase (ALT)

aspartic acid transferase (AST)

albumin/globulin and lactic dehydrogenase (LDH) contents were detected with a full automatic biological analyzer. EA (2～14 Hz

1 mA) was applied to bilateral “Neiguan"(PC 6) for 150 min. AG

a selective inhibitor of iNOS was given to the rats intraperitoneally. Results: In comparison with control group

serum ALT

AST and LDH levels of model

AG

EA and EA+AG groups increased significantly from 30 min on after injection of LPS and D GalN (P<0.05～0.01)

suggesting an injury of the liver tissues. Compared with model group

serum ALT

AST and LDH levels of EA and EA+AG groups decreased considerably from 60 min (ALT and AST) or 30 min (LDH) on after EA. No significant differences were found between model group and AG group in ALT

AST and LDH levels

and no apparent changes were found in serum A/G values in the five groups (P>0.05). Immunohistochemical staining displayed that the ratios of eNOS immunoreaction (IR) positive hepatocytes/the observed total hepatocytes of model

AG

EA and EA+AG groups were significantly lower than that of control group (P<0.05～0.01)

and that of EA group was considerably higher than those of AG and model groups (P<0.01). While the ratios of iNOS IR positive hepatocytes of model

AG

EA and EA+AG groups were significantly higher than that of control group

and those of AG

EA and EA+AG groups were significantly lower than that of model group (P<0.05～0.01). Compared with AG group

the ratios of iNOS positive cells of EA and EA+AG groups were strikingly higher (P<0.05～0.01). It indicated that after liver injury

iNOS expression was upregulated and eNOS expression downregulated

AG had a suppressive effect on both iNOS (stronger in the effect) and eNOS (weaker)

and EA could downregulate iNOS expression and upregulate eNOS expression. Conclusion: EA of Neiguan Point can reduce D GalN LPS induced acute hepatocyte injury probably by way of adjusting NOS protein expression.