Objective: To observe the effect of electroacupuncture(EA) of "Baihui"(GV 20) and "Taixi"(KI 3) on changes of behavior activities and concentrations of monoamines transmitters in hypothalamic tissues of rats with chronic fatigue syndrome(CFS).Methods: Thirty-six male Wistar rats were evenly randomized into control

model and EA groups.CFS model was established by intramuscular injection of hydrocortisone(20 mg/kg

1 time/d

9 d) and withdrawal of hydrocortisone

and forced swim in cool water(30 min/1 time/d

21 days).EA(2/20 Hz

9 V) was applied to "Baihui"(GV 20) and "Taixi"(KI 3) for(30 min)

once daily and continuously for 21 days.Open-field test

rat's tail suspension immobility test and exhausted swimming test were used to check the rats' behavioral changes and double antibody sandwich enzyme-linked immunosorbnent assay(ABC-ELISA) was used to determine concentrations of noradrenaline(NE)

serotonin(5-HT) and dopamine(DA) in hypothalamus tissues.Results: Compared with control group

the crossing and rearing activity times

the duration of tail-suspension immobility and the duration of exhausted swim

and the contents of NE

5-HT and DA of hypothalamus tissues in model group were all significantly lower(P<0.05);while the values of the abovementioned 7 indexes of EA group were all significantly higher than those of model group(P<0.05).No significant differences were found between EA group and control group in these 7 indexes(P>0.05).It showed that EA could improve behavioral activities of CFS rats and raise the contents of monoamines neurotransmitters in the hypothalamus.Conclusion: EA of "Baihui"(GV 20) and "Taixi"(KI 3) can ameliorate behavior activities of CFS rats and reverse the declined contents of NE

5-HT and DA of hypothalamus tissue.