艾灸“肺俞”“膏肓”对博莱霉素A5诱导肺纤维化大鼠Ⅰ/Ⅱ型细胞因子平衡调节的影响

李戎; 周蜜娟; 李翠霞; 李富红; 李文军; 蔡永宁; 李志刚; 罗全安; 刘明芳

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艾灸“肺俞”“膏肓”对博莱霉素A5诱导肺纤维化大鼠Ⅰ/Ⅱ型细胞因子平衡调节的影响

更新时间：2023-08-11

- 艾灸“肺俞”“膏肓”对博莱霉素A5诱导肺纤维化大鼠Ⅰ/Ⅱ型细胞因子平衡调节的影响
- Influence of Moxibustion of "Feishu"(BL 13) and "Gaohuangshu"(BL 43) on the Regulation of Th1/Th2 Cytokines in BLM_(A5)-induced Pulmonary Fibrosis Rats
- 针刺研究 2005年第3期页码：164-166
- 作者机构：
  
  成都中医药大学
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(No.30472236);国家中医药管理局专项课题(No.04-05JP54)
- DOI：
  中图分类号：
- 纸质出版日期：2005
- 稿件说明：
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李戎, 周蜜娟, 李翠霞, 等. 艾灸“肺俞”“膏肓”对博莱霉素A5诱导肺纤维化大鼠Ⅰ/Ⅱ型细胞因子平衡调节的影响[J]. 针刺研究, 2005,(3):164-166.

Influence of Moxibustion of "Feishu"(BL 13) and "Gaohuangshu"(BL 43) on the Regulation of Th1/Th2 Cytokines in BLM_(A5)-induced Pulmonary Fibrosis Rats[J]. Acupuncture research, 2005, (3): 164-166.
李戎, 周蜜娟, 李翠霞, 等. 艾灸“肺俞”“膏肓”对博莱霉素A5诱导肺纤维化大鼠Ⅰ/Ⅱ型细胞因子平衡调节的影响[J]. 针刺研究, 2005,(3):164-166. DOI：

Influence of Moxibustion of "Feishu"(BL 13) and "Gaohuangshu"(BL 43) on the Regulation of Th1/Th2 Cytokines in BLM_(A5)-induced Pulmonary Fibrosis Rats[J]. Acupuncture research, 2005, (3): 164-166. DOI：

摘要

目的:研究艾灸“肺俞”“膏肓”对博莱霉素(BLM)所致肺纤维化大鼠肺组织Ⅰ/Ⅱ型细胞因子平衡调节的影响

探索针灸阻抑肺纤维化的效应机制。方法:SD大鼠随机分为:空白组、模型组、艾灸组、泼尼松组。气管内注入博莱霉素制作大鼠肺纤维化模型

造模后7 d开始治疗

以5 mg左右艾绒灸其双侧“肺俞”“膏肓”

每穴3壮

每天1次

10 d为1疗程

疗程间休息1 d

3个疗程后处死动物

采用酶联免疫吸附法测定肺组织内Ⅰ型细胞因子IL-2I、L-12和Ⅱ型细胞因子IL-4、IL-5含量。结果:造模后大鼠肺组织内Ⅰ型细胞因子含量降低

Ⅱ型细胞因子含量升高(均P<0.01);艾灸及泼尼松治疗能够使大鼠肺组织内Ⅰ型细胞因子含量升高

Ⅱ型细胞因子含量降低

与模型组比较具有统计学差异(P<0.05

0.01);艾灸组与泼尼松组比较

各指标均无统计学差异(P>0.05)。结论:艾灸“肺俞”“膏肓”能升高BLMA5诱导肺纤维化大鼠肺组织中Ⅰ型细胞因子IL-2I、L-12的含量

降低Ⅱ型细胞因子IL-4I、L-5的含量

提示调节Ⅰ/Ⅱ型细胞因子的平衡可能是艾灸阻抑肺纤维化发生的机理之一。

Abstract

Objectives: To observe the effect of moxibustion of "Feishu"(BL 13) and "Gaohuangshu"(BL 43) on the balance of T helper1/T helper2(Th1/Th2) cytokines in BLM_(A5)-induced pulmonary fibrosis rats

and to explore its underlying mechanism.Methods: Twenty-four SD rats were evenly randomized into control

model

moxibustion

and medication groups.Pulmonary fibrosis model was established by injection of Bleomycin via trachea(5 mg/kg).Moxibustion(3 cones/acupoint) was applied to bilateral "Feishu"(BL 13) and "Gaohuangshu"(BL 43)

once daily

with 10 days being a therapeutic course and an interval of one day

3 courses altogether.Rats of medication group were fed with Prednisone(2.5 mg/kg)

once daily and continuously for 30 days.At the end of experiments

the rats were killed for taking out the left mid-lung tissues to be processed into homogenate samples.Cytokines such as IL-2

IL-12

IL-4 and IL-5 were detected with enzyme linked immunosorbent assay according to instructions of reagent kits.Results: In comparison with control group

IL-2 and IL-12 contents of the lung tissue were significantly lower

while IL-4 and IL-5 considerably higher in model group(P<0.01);whereas compared with model group

IL-2 and IL-12 contents were significantly higher(P<0.05

0.01)

IL-4 and IL-5 levels significantly lower in moxibustion and medication group(P<0.01).No significant differences were found between moxibustion group and medication group in the 4 indexes(P>0.05).It indicated that in BLM_(A5)-induced pulmonary fibrosis rats

Th1 cytokines IL-2 and IL-12 levels in the lung tissue decreased markedly

and Th2 cytokines IL-4 and IL-5 levels increased abnormally

both moxibustion and Prednisone could regulate changes of Th1 and Th2 cytokines.Conclusion: Moxibustion of "Feishu"(BL 13) and "Gaohuangshu"(BL 43) can raise the contents of Th1 cytokines(IL-2 and IL-12) and lower those of Th2 cytokines(IL-4 and IL-5)

suggesting that moxibustion induced balance between Th1 and Th2 cytokines may contribute to its action in resisting pulmonary fibrosis.

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