电针对VD大鼠脑微血管内皮细胞一氧化氮合酶表达的影响

张会珍; 房家毅; 佘延芬; 马小顺; 田义龙; 王鑫国

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电针对VD大鼠脑微血管内皮细胞一氧化氮合酶表达的影响

更新时间：2023-08-11

- 电针对VD大鼠脑微血管内皮细胞一氧化氮合酶表达的影响
- Effect of Electroacupuncture on Nitric Oxide Synthase Expression of Endothelial Cells inside the Brain Micrangium in Vascular Dementia Rats
- 针刺研究 2005年第3期页码：150-153
- 作者机构：
  
  河北医科大学中医学院
- 作者简介：
- 基金信息：
  
  河北省科技厅课题(No.042761388)
- DOI：
  中图分类号：
- 纸质出版日期：2005
- 稿件说明：
移动端阅览
张会珍, 房家毅, 佘延芬, 等. 电针对VD大鼠脑微血管内皮细胞一氧化氮合酶表达的影响[J]. 针刺研究, 2005,(3):150-153.

Effect of Electroacupuncture on Nitric Oxide Synthase Expression of Endothelial Cells inside the Brain Micrangium in Vascular Dementia Rats[J]. Acupuncture research, 2005, (3): 150-153.
张会珍, 房家毅, 佘延芬, 等. 电针对VD大鼠脑微血管内皮细胞一氧化氮合酶表达的影响[J]. 针刺研究, 2005,(3):150-153. DOI：

Effect of Electroacupuncture on Nitric Oxide Synthase Expression of Endothelial Cells inside the Brain Micrangium in Vascular Dementia Rats[J]. Acupuncture research, 2005, (3): 150-153. DOI：

摘要

目的:观察一氧化氮合酶(iNOS)在血管性痴呆(VD)大鼠脑微血管内皮细胞中的表达情况及电针对其的影响。方法:采用夹闭再通大鼠双侧颈总动脉的方法建立缺血再灌注损伤性VD模型

电针组用G-6805电针治疗仪

以疏密波刺激“百会”“大椎”“足三里”“膈俞”

治疗20 min

每日1次

连续治疗7 d。利用SP免疫组织化学染色方法

观察脑微血管内皮细胞iNOS的活性

并与假手术组、模型组做比较。结果:电针组大鼠脑微血管内皮细胞iNOS的活性较模型组减弱

与模型组比较有显著性差异(P<0.01)。结论:电针可抑制VD大鼠脑微血管内皮细胞iNOS的活性

对脑缺血再灌注损伤具有脑保护作用。

Abstract

Objective: To observe the expression of inducible nitric oxide synthase(iNOS) in the endothelial cells inside the cerebral micrangium and the effect of electroacupuncture(EA) in vascular dementia(VD) rats.Methods: Thirty Wistar rats were evenly randomized into sham-operation(control)

model and EA groups.VD model was established by occlusion of the bilateral common carotid artery and reperfusion plus intraperitoneal injection of sodium nitroprusside(2.5 mg/kg).In EA group

EA(frequency 5/45 Hz

strength 1.5-3 V) was applied to "Baihui"(GV 20)

"Dazhui"(GV 14)

"Zusanli"(ST 36) and "Geshu"(BL 17) for 20 minutes

once a day

continuously for 7 days.The activity of iNOS in the endothelial cells of right cerebral cortex tissue was assayed with immunohistochemical method.The rat's memory-learning ability was detected by using step-down test(passive avoidance reaction

latency and number of errors of step-down).Results: In comparison with control group

the latency of step-down from the platform of model group shortened significantly

and the number of errors of model group increased remarkably.While compared with model group

the latency of step-down of EA group increased significantly and the number of errors decreased markedly(P<0.01).No significant differences were found between control and EA groups in these two indexes(P>(0.05).) Very weak immunoreaction(IR) of iNOS was found only in fewer slices of the brain tissues in control group;while in majority of the slices of model group

moderate IR and stronger IR were found.In comparison with model group

mild IR of iNOS in EA group was predominant while moderate and strong IR were significantly fewer in the number of slices.There was a significant difference between model group and EA group(P<0.01)

suggesting that after EA

the activity of iNOS in most endothelial cells in cerebral micrangium reduced obviously.Conclusion: Electroacupuncture can obviously reduce the activity of iNOS in the endothelial cells of cerebral micrangium in VD rats

and protect the brain from ischemia/reperfusion injury.

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