目的:观察传统“热补”针法对实验性关节炎家兔的镇痛效应和探求其中枢作用机制。方法:以卵蛋白诱导的关节炎家兔为疼痛模型

连续治疗6 d后比较药物组、捻针组、电针组和热补组关节炎家兔关节局部痛阈和脑脊液中-βEP和CCK-8的含量。结果:治疗后各治疗组痛阈和-βEP含量均升高

热补组-βEP含量明显高于药物组和捻针组(P<0.01)

但不及电针明显(P<0.05);各治疗组CCK-8含量均向正常水平恢复

热补组CCK-8含量比药物组和捻针组恢复明显(P<0.01)

但不及电针组恢复显著(P<0.01)。结论:“热补”针法的镇痛效应和捻转针法、药物(痹冲剂)相同

但不及电针的镇痛效应;升高脑脊液中-βEP含量和促使CCK-8含量向正常水平恢复

可能是其镇痛的中枢机制之一。Objective: To observe the effect of heat-reinforcing needling manipulation on inflammatory pain and explore its analgesic mechanism in experimental rheumatoid arthritis rabbits.Methods: Fifty-two rabbits were randomized into control(n=9)

model(n=9)

medication(n=9)

needle-twirling(n=9)

electroacupuncture(EA

n=9) and heat-reinforcing(n=9) groups.Rheumatoid arthritis model was established by injecting mixed solution of egg-albumin((4 mg/mL)) and equal volume of complete Freund's adjuvant(CFA) into the subcutaneous tissue(6 points around the shoulder

(0.2 mL/point))

14 days later

the injection was repeated once again

another 6 days later

egg-albumin((0.4 mL)

(20 mg/mL)) solution was injected into the bilateral knee-joints.Rabbits of medication group was fed with "Wangbi Granule"(■痹冲剂) 0.56 g/time(t.i.d.)."Zusanli"(ST 36) and "Hegu"(LI 4) were punctured and stimulated by twirling the needle leftward and rightward for(1 min)(needle retention(30 min))

or electrically((4/20 Hz)

(0.5 ms) pulse-duration

2.4-(3.1 mA) and duration of(30 min)) or by manipulating the needle with heat-reinforcing method for(1 min)(needle-retention(30 min)).The treatment was conducted once daily and continuously for 6 days.The pain threshold(leg-withdrawal latency) was detected

and β-EP and CCK-8 contents in cerebrospinal fluid were assayed with radioimmunoassay.Results: Compared with control group

the pain threshold and CCK-8 contents of model group lowered significantly(P<0.01)

and β-EP of model group increased significantly(P<0.05);while in comparison with model group

the pain threshold and CCK-8 contents of medication

needle-twirling

EA and heat-reinforcing groups increased significantly(P<0.05

0.01)

and β-EP contents of the 4 treatment groups increased further considerably(P<0.05

0.01);and the effects of EA group were significantly superior to those of the other 3 treatment groups in raising pain threshold

and the contents of β-EP and CCK-8(P<0.05

0.01).The effects of heat-reinforcing group were significantly superior to those of needle-twirling and medication groups in raising β-EP and CCK-8 levels(P<0.01).Conclusion: Heat-reinforcing needling manipulation

Chinese herbal medicine

EA and needle-twirling method all have an analgesic effect in rheumatoid arthritis rats

which is probablyrelated to their effects in raising cerebral βEP and CCK-8 contents.