针药结合对溃疡性结肠炎大鼠结肠粘膜IFN-γ、IL-12表达的影响

施茵; 吴焕淦; 汪崇淼; 施征; 华雪桂; 刘慧荣

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针药结合对溃疡性结肠炎大鼠结肠粘膜IFN-γ、IL-12表达的影响

更新时间：2023-08-11

- 针药结合对溃疡性结肠炎大鼠结肠粘膜IFN-γ、IL-12表达的影响
- Effects of Acupuncture Combined with Medicine on the Expression of IFN-γ and IL-12 of Colonic Mucous Membrane in Ulcerative Colitis Rats
- 针刺研究 2005年第4期页码：215-218
- 作者机构：
  
  1. 上海市针灸经络研究所
  2. 上海市第六人民医院
- 作者简介：
- 基金信息：
  
  上海市卫生局中医药重大研究项目[沪卫中医(1999)39号];上海市重点学科建设项目资助(T0302)
- DOI：
  中图分类号：
- 纸质出版日期：2005
- 稿件说明：
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施茵, 吴焕淦, 汪崇淼, 等. 针药结合对溃疡性结肠炎大鼠结肠粘膜IFN-γ、IL-12表达的影响[J]. 针刺研究, 2005,(4):215-218.

SHI Yin, WU Huan-gan, WANG Chong-miao, et al. Effects of Acupuncture Combined with Medicine on the Expression of IFN-γ and IL-12 of Colonic Mucous Membrane in Ulcerative Colitis Rats[J]. Acupuncture research, 2005, (4): 215-218.
施茵, 吴焕淦, 汪崇淼, 等. 针药结合对溃疡性结肠炎大鼠结肠粘膜IFN-γ、IL-12表达的影响[J]. 针刺研究, 2005,(4):215-218. DOI：

SHI Yin, WU Huan-gan, WANG Chong-miao, et al. Effects of Acupuncture Combined with Medicine on the Expression of IFN-γ and IL-12 of Colonic Mucous Membrane in Ulcerative Colitis Rats[J]. Acupuncture research, 2005, (4): 215-218. DOI：

摘要

目的:探讨针药结合对溃疡性结肠炎(UC)大鼠结肠粘膜IFN-γI、L-12表达的调节机制。方法:在成功建立大鼠溃疡性结肠炎模型的基础上

采用电针“足三里”穴结合西药柳氮磺胺吡啶(SASP)治疗

并与单纯电针、西药、模型及正常组进行对照

以免疫组化和HE染色法观察UC大鼠结肠粘膜IFN-γ、IL-12免疫阳性细胞的表达和组织形态学变化。结果:与正常组相比

模型组大鼠结肠粘膜IFN-γI、L-12免疫阳性细胞表达出现极显著升高(P<0.01)

针药、电针及西药组IFN-γ表达则见不同程度的降低(P<0.05

0.01)

其中针药组表达接近于正常组水平;而IL-12表达

针药、电针及西药组均较模型组有极显著性降低(P<0.01)。同时

各治疗组对UC大鼠结肠粘膜的组织形态学变化均有一定程度的改善。结论:UC大鼠结肠粘膜IFN-γI、L-12的异常表达是溃疡性结肠炎的发病机制之一。针药结合可能通过有效抑制UC结肠粘膜IFN-γI、L-12的表达达到其治疗目的。

Abstract

Objective: To study the mechanism of electroacupuncture(EA) combined with medicine in adjusting the expression of mucosal IFN-γ and IL-12 in ulcerative colitis rats.Methods: Fifty SD rats were averagely randomized into normal control

model

medication and EA+medication groups.Ulcerative colitis model was established by using immunity(intramuscular injection of tissue antigen of the ulcerative colitis patient's colonic mucous and Freund adjuvant) plus local stimulation(enema of 3% formalin) methods.For rats of EA and EA+medication groups

EA(2 Hz

4 mA

continuous electric pulses) was applied to bilateral "Zusanli"(ST 36) for 20 min

once daily

continuously for 10 days.For rats of medication and EA+medication groups

Salicylazosulfapyridine(SASP) solution(3 mL) was given by intra-gastric perfusion

twice daily

continuously for 10 days.At the end of experiments

the rats were killed for sampling colonic tissues.The tissue was embedded with paraffin and stained with HE and immunohistochemical techniques separately for observing morphological changes of the colonic mucous membrane and expression of IFN-γ and IL-12.Results: Compared with normal group

the immuno-reactive positive cells of IFNγ and IL-12 increased significantly in model group.In comparison with model group

the expression of IFN-γ and IL-12 in EA

medication and EA+medication groups were significantly lower(P<0.05

0.01)

indicating that EA

medication and EA plus medication all could suppress the expression of IFN-γ and IL-12 in the colonic mucous membrane.No significant differences were found among EA and medication and EA+medication groups in these two indexes(P>0.05)

but the values of IFN-γ and IL-12 in EA+medication group were closer to those of normal control group.In model group

tissue lesion such as large area of erosion

ulceration

congestion/ hemorrhage

edema

inflammatory cell infiltration

partial colonic mucous necrosis

etc was seen

while in EA

medication and EA+medication groups

these changes were lighter.Conclusion: Acupuncture combined with medicine therapy can effectively suppress experimental ulcerative colitis induced abnormal expression of IFN-γ and IL-12

which may contribute to its effect in relieving ulcerative colitis.

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