电针对脑缺血再灌注大鼠大脑皮层去甲肾上腺素及细胞凋亡的影响

邹晓静; 施静; 刘敬; 李伶俐; 刘晓春

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电针对脑缺血再灌注大鼠大脑皮层去甲肾上腺素及细胞凋亡的影响

更新时间：2023-08-11

- 电针对脑缺血再灌注大鼠大脑皮层去甲肾上腺素及细胞凋亡的影响
- Effect of Electroacupuncture on NE and Neuronal Apoptosis in the Cerebral Cortex in Rats with Cerebral Ischemia-reperfusion
- 针刺研究 2005年第4期页码：203-207
- 作者机构：
  
  华中科技大学同济医学院神经生物学系
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助(No.30472234);教育部博士点基金资助(No.2003487069)
- DOI：
  中图分类号：
- 纸质出版日期：2005
- 稿件说明：
移动端阅览
邹晓静, 施静, 刘敬, 等. 电针对脑缺血再灌注大鼠大脑皮层去甲肾上腺素及细胞凋亡的影响[J]. 针刺研究, 2005,(4):203-207.

ZOU Xiao-jing, SHI Jing, LIU Jing, et al. Effect of Electroacupuncture on NE and Neuronal Apoptosis in the Cerebral Cortex in Rats with Cerebral Ischemia-reperfusion[J]. Acupuncture research, 2005, (4): 203-207.
邹晓静, 施静, 刘敬, 等. 电针对脑缺血再灌注大鼠大脑皮层去甲肾上腺素及细胞凋亡的影响[J]. 针刺研究, 2005,(4):203-207. DOI：

ZOU Xiao-jing, SHI Jing, LIU Jing, et al. Effect of Electroacupuncture on NE and Neuronal Apoptosis in the Cerebral Cortex in Rats with Cerebral Ischemia-reperfusion[J]. Acupuncture research, 2005, (4): 203-207. DOI：

摘要

目的:探讨电针对脑缺血再灌注神经元保护的作用及其机制。方法:SD大鼠随机分为对照组、缺血再灌注组及电针组

以双侧颈总动脉夹闭方法建立脑缺血模型

用流式细胞仪检测细胞凋亡和Bcl-2及Bax蛋白表达的变化

并用荧光分光法检测皮层去甲肾上腺素(norepinephrine

NE)含量的变化。电针穴位选择“水沟”“承浆”穴。结果:①大鼠脑缺血再灌注48 h后

细胞凋亡率明显高于对照组(P<0.05)

Bcl-2蛋白表达在皮层明显降低

Bax/Bcl-2比值较对照组明显升高(P<0.05);电针治疗后

Bax的表达减少

Bcl-2的表达增加

Bax/Bcl-2比值明显下降(P<0.05)

细胞凋亡率受到抑制(P<0.05)。②大鼠脑缺血再灌注3 min后NE含量在皮层明显升高

与对照组相比有显著性差异(P<0.05);脑缺血再灌注48 h后

皮层NE含量明显回落

与对照组相比无显著性差异;电针治疗后

脑缺血再灌注早期(即缺血再灌3 min后)脑组织内NE升高的现象出现逆转

与模型组比较有显著性差异(P<0.05)。结论:脑缺血再灌注早期大鼠脑NE出现变化

此变化可能与神经元的凋亡发生有关。电针能逆转脑缺血再灌导致的NE的异常变化

并调节Bax/Bcl-2的表达水平

抑制神经元凋亡的发生。

Abstract

Objective: To investigate the underlying neurobiological mechanism of the protective effect of electroacupuncture(EA) on cerebral neurons during cerebral ischemia and reperfusion(CI/R).Methods: Fifteen SD rats were evenly randomized into control

model(CI/R 48 h) and EA groups.CI/R model was established by occlusion of the bilateral carotid arteries and reperfusion.The neuronal apoptosis and expression of Bcl-2 and Bax proteins of the cerebral cortex in each group were detected by flow cytometer.Additionally

other 75 SD rats were evenly randomized into control

CI/R-3 min

CI/R-3 min+EA

CI/R-48 h and CI/R-48 h+EA groups.NE concentration of cerebral cortex was detected by fluorescence spectrometer.EA(4-16 Hz

1-3 V) was applied to "Shuigou"(GV 26) and "Chengjiang"(RN 24) for 30 min before CI/R.Results: In comparison with control group

the number of neurons in the cerebral cortex in phase sub-G_1 and the apoptotic rate in CI/R-48 h group were significantly higher(P<0.05);while comparison between CI/R-48 h and CI/R-48 h+EA groups showed that both the number of neurons in phase sub-G_1 and the apoptotic rate of the later group were significantly lower than those of CI/R-48 h group(P

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