电针对大鼠胃肠动力障碍的调整作用及其神经化学机制

雷君; 李琴; 李国成; 茹立强

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电针对大鼠胃肠动力障碍的调整作用及其神经化学机制

更新时间：2023-08-11

- 电针对大鼠胃肠动力障碍的调整作用及其神经化学机制
- Regulatory Effects of Electroacupuncture of "Zusanli"(ST 36) on Gastrointestinal Motility Disorder in Rats and Its Related Neurochemical Mechanism
- 针刺研究 2005年第3期页码：131-137
- 作者机构：
  
  1. 华中科技大学同济医学院神经生物学系
  2. 华中科技大学同济医学院神经生物学系,武汉,430030
- 作者简介：
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- 纸质出版日期：2005
- 稿件说明：
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雷君, 李琴, 李国成, 等. 电针对大鼠胃肠动力障碍的调整作用及其神经化学机制[J]. 针刺研究, 2005,(3):131-137.

Regulatory Effects of Electroacupuncture of "Zusanli"(ST 36) on Gastrointestinal Motility Disorder in Rats and Its Related Neurochemical Mechanism[J]. Acupuncture research, 2005, (3): 131-137.
雷君, 李琴, 李国成, 等. 电针对大鼠胃肠动力障碍的调整作用及其神经化学机制[J]. 针刺研究, 2005,(3):131-137. DOI：

Regulatory Effects of Electroacupuncture of "Zusanli"(ST 36) on Gastrointestinal Motility Disorder in Rats and Its Related Neurochemical Mechanism[J]. Acupuncture research, 2005, (3): 131-137. DOI：

摘要

目的:探讨针刺对胃肠运动功能的调整作用及可能的神经化学机制。方法:Wistar大鼠64只

随机分为正常对照组、胃肠动力障碍组、胃肠动力障碍+西药治疗组和胃肠动力障碍+电针治疗组。采用夹尾刺激激怒法制成大鼠胃肠动力障碍模型

用应力传感器记录大鼠胃和十二指肠消化间期移行性复合运动(MMC)

采用组织化学技术观测胃、十二指肠肌间神经丛乙酰胆碱酯酶(AChE)、一氧化氮合酶(NOS)阳性神经元的变化。结果:①MMC的改变:与对照组比较

胃肠动力障碍组胃-肠MMC周期、Ⅰ、Ⅱ相延长

Ⅲ相缩短(P<0.01)

Ⅲ相频率减慢、幅度下降(P<0.01)

胃Ⅲ相发生率降低(P<0.01)

胃-肠平均协调收缩率显著下降(P<0.01);西药治疗组、电针治疗组与胃肠动力障碍组比较

胃-肠MMC周期、Ⅰ、Ⅱ相缩短

Ⅲ相延长(P<0.05

0.01)

Ⅲ相频率加快、幅度上升(P<0.01)

胃Ⅲ相发生率升高(P<0.01)

胃-肠平均协调收缩率显著升高(P<0.01);与西药组比较

电针组各指标的改变较明显(P<0.05

0.01)。②胃窦、十二指肠肌间神经丛AChE阳性神经元表达:与对照组比较

胃肠动力障碍组显著减少(P<0.01);与胃肠动力障碍组比较

西药治疗组、电针治疗组显著增加(P<0.01)。③胃窦、十二指肠肌间神经丛NOS阳性神经元表达:与对照组比较

胃肠动力障碍组显著增加(P<0.01);与胃肠动力障碍组比较

西药治疗组、电针治疗组显著减少(P<0.01)。结论:①胃、十二指肠协调运动障碍可能是胃肠动力障碍大鼠发病机制之一;②胆碱能神经、氮能神经紊乱可能是胃肠动力障碍大鼠胃、十二指肠协调运动障碍的神经化学基础;③针刺能改善胃肠动力障碍大鼠胃、十二指肠运动协调性

效应优于胃肠动力药多潘立酮

其作用机理可能与调节ACh、NO神经递质的释放有关。

Abstract

Objective: To investigate the regulatory effect of electroacupuncture(EA) on gastrointestinal motility and its underlying neurochemical mechanism.Methods: A total of 64 Wistar rats were randomly divided into control

model

medication(Domperidone

31.5 mL/kg/d

intragastric perfusion

7 days altogether) and EA groups

with 16 cases in each group.Gastrointestinal motility disorder model was established by pinching the rat's tail repeatedly with a forceps.EA(1-5 V

4-16 Hz

dense-sparse waves) was applied to bilateral "Zusanli"(ST 36) for 50 min

once daily

one week altogether.The interdigestive migrating motor complex(MMC) waves delivered by using strain gauge electrodes fixed to the subserous layer of gastric antrum and duodenal wall were recorded.Acetylcholinesterase(AChE) and nitric oxide synthase(NOS) activity in gastroduodenal myenteric plexus was assayed by using ferric cupric cyanide direct coloration method and NADPH-diaphorase method respectively.Results: ① MMC: Compared with control group

the cycle

duration of phase Ⅰ and Ⅱ of MMC of the stomach-duodenum in model group prolonged remarkably(P<0.01)

while the duration

frequency

amplitude and the incidence of phase Ⅲ

and the coordinated contraction rate of the gastric antrum-duodenum in model group all decreased significantly(P<0.01);in comparison with model group

the cycle

duration of phase Ⅰand Ⅱ of MMC of both stomach and duodenum in medication and EA groups shortened significantly(P<0.05

0.01)

while the duration

frequency

amplitude and incidence of phase Ⅲ as well as the coordinated contraction of the stomach-duodenum in medication and EA groups increased significantly(P<0.01);and the effects of EA were significantly superior to those of medication in the aforementioned indexes(P<0.05

0.01).② AChE: Compared with control group

the area density(AD) and optical density(OD) values of myenteric plexus in model group decreased significantly(P

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