Objective:To study the scientific basis of specific correlation between the meridian and the Zang-or Fu-organ by using gene differential expression(gene-chip technique)in the rat.Methods:Twenty-one SD rats were randomly and evenly divided into control group

sham-myocardial ischemia(MI)group

MI group

electroacupuncture(EA)-Heart-Meridian(HT)group

EA-Lung-Meridian(LU)group and EA-Small-Intestine-Meridian(SI)group.EA(frequency 2 Hz

stimulating strength 5 V

pulse duration 300 μs)was applied to “Shenmen”(HT 7)-“Tongli”(HT 5)(Heart Meridian)

“Yanglao”(SI 6)-“Zhizheng”(SI 7)(Small Intestine Meridian)

“Taiyuan”(LU 9)-“Lieque”(LU 7)(Lung Meridian)for 20 min

once daily and continuously for 3 days.Myocardial ischemia model was duplicated by occlusion of the anterior descending branch of the left coronary artery

and myocardial tissue of the left ventricle was taken 3 days after EA treatment for analyzing the differentially expressed genes in different groups with rat whole gene U 230 sequence chips(offered by Affymetrix Company).Results:Compared with model group

among the differentially expressed genes being twofolds and above

the up-regulated and the down-regulated were 20 and 70 in EA-HT group

18 and 26 in EA-SI group

and 14 and 20 in EA-LU group separately.Few same genes with the same differential expression were found among the 3 EA groups.Conclusion:There is exactly a molecular basis in the correlation between Heart Meridian and the heart

and EA of Heart Meridian

Small Intestine Meridian and Lung Meridian have their own special signaling pathways.