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1. 浙江中医药大学第三临床医学院
2. 浙江中医药大学第三临床医学院,杭州,310053
纸质出版日期:2007
移动端阅览
方剑乔, 刘芳, 邵晓梅, 等. 电针对角叉菜胶致炎大鼠的抗炎效应及对白细胞介素-1β、肿瘤坏死因子-α的影响[J]. 针刺研究, 2007,(4):224-228.
FANG Jian-qiao, LIU Fang, SHAO Xiao-mei, et al. Effect of Electroacupuncture on Carrageenan-induced Inflammation,IL-1β and TNF-α Concentrations and Their mRNA Expressions in Toe Tissue in Rats[J]. Acupuncture research, 2007, (4): 224-228.
方剑乔, 刘芳, 邵晓梅, 等. 电针对角叉菜胶致炎大鼠的抗炎效应及对白细胞介素-1β、肿瘤坏死因子-α的影响[J]. 针刺研究, 2007,(4):224-228. DOI:
FANG Jian-qiao, LIU Fang, SHAO Xiao-mei, et al. Effect of Electroacupuncture on Carrageenan-induced Inflammation,IL-1β and TNF-α Concentrations and Their mRNA Expressions in Toe Tissue in Rats[J]. Acupuncture research, 2007, (4): 224-228. DOI:
目的:观察电针对角叉菜胶致炎大鼠的治疗作用
并探讨电针抗急性炎症的部分机理。方法:正常Wistar大鼠分别在电针、消炎痛和罗非昔布预处理5 d后致角叉菜胶急性炎症模型。电针刺激"曲池"穴
刺激参数为:连续波
频率2 Hz
强度5 mA
时间30 min
每天1次
连续5 d;消炎痛和罗非昔布预处理分别以7.5 mg/kg灌胃
每天1次
连续5 d;另一组大鼠在相同造模后进行即刻电针处理(参数同电针预处理组)。以毛细管放大法检测足跖肿胀度
ELISA法检测炎症足爪PGE2、IL-1β和TNF-α水平
RT-PCR法检测足爪炎症组织IL-1βmRNA、TNF-αmRNA表达。结果:电针可有效抑制角叉菜胶致炎大鼠足跖肿胀
尤以造模后2、3 h最为显著;可降低大鼠炎症足爪组织中的PGE2水平;不同时段电针介入治疗均能下调炎症足爪组织中IL-1β和TNF-α水平
但以造模后治疗为佳;电针对足爪炎症组织IL-1βmRNA、TNF-αmRNA表达产生不同程度的下调作用。结论:电针对角叉菜胶致炎大鼠急性炎症具有良好的抗炎效应
并通过抑制局部促炎症因子(如IL-1β、TNF-α)的生成与表达而控制炎症的发展。
Objective: To observe the anti-inflammatory effects of electroacupuncture(EA) at "Quchi"(LI 11) and its influence on IL-1β
TNF-α concentrations and mRNA expressions in carrageenan(CAR)-induced inflammation rats
so as to reveal partial mechanisms of EA in relieving acute inflammation.Methods: Sixty male Wistar rats were divided into control
model
Indomethacin(IND
7.5 mg/kg
oral perfusion)
Rofecoxib(7.5 mg/kg
oral perfusion)
EA-pre-treatment(EA was given once daily for 5 days before modeling)
EA-post-treatment(EA was given once after modeling) groups
with 10 cases in each.EA(2 Hz
5 mA and persistent waves) was applied to "Quchi"(LI 11) for 30 min each time.Arthritis model was established by subcutaneous injection of 1% CAR
0.1 mL into the right hind paw.Paw edema degree of the affected hind paw was measured with a foot-volume surveyor.After processing samples of the affected paw toes(including smashing
centrifugalization
etc)
concentrations of prostaglandin(PG) E2
interleukin(IL)-1β
tumor necrosis factor(TNF)-α were measured by enzyme linked immunosorbent assay(ELISA).Expressions of IL-1β mRNA and TNF-α mRNA in the local inflammatory tissues were detected by reverse-transcription polymerase chain reaction(RT-PCR) assay.Results: Compared with model group
the affected hind-paw-volume decreased significantly from 2 h on in EA-post-treatment group and from 1 h on in IND group after injection of CAR.Compared with control group
PGE2
IL-1β and TNF-α contents in the toe tissue of model group increased significantly;while in comparison with model group
PGE2 and IL-1β levels in EA-post-treatment group
IL-1β level in IND group decreased significantly(P<0.05)
and TNF-α level in IND group increased significantly.No significant differences were found between model group and Rofecoxib and EA-pre-treatment groups in most time-courses of the hind-paw-volume
between model and IND
Rofecoxib and EA-pre-treatment groups in PGE2
between model and EA-pre-treatment groups in IL-1β
and between model and Rofecoxib
EA-pre-treatment and EA-post-treatment groups in TNF-α contents(P>0.05).Compared with model group
IL-1β mRNA expression of the local inflammatory tissues in IND and EA-post-treatment groups was down-regulated
and TNF-α mRNA expression in Rofecoxib and two EA groups down-regulated slightly.Conclusion: EA possesses anti-inflammatory effect in acute arthritis rats
which is closely related to its effects in reducing the secretion of PGE2
IL-1β and TNF-α and the expression of IL-1β mRNA and TNF-α mRNA.
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