Effects of Electroacupuncture on the Expression of Epidermal Growth Factor Receptor and Glial Fibrillary Acidic Protein after Spinal Cord Injury in Rats
PENG Bin, MENG Xian-fang, LI Man, et al. Effects of Electroacupuncture on the Expression of Epidermal Growth Factor Receptor and Glial Fibrillary Acidic Protein after Spinal Cord Injury in Rats[J]. Acupuncture research, 2007, (4): 219-223.
PENG Bin, MENG Xian-fang, LI Man, et al. Effects of Electroacupuncture on the Expression of Epidermal Growth Factor Receptor and Glial Fibrillary Acidic Protein after Spinal Cord Injury in Rats[J]. Acupuncture research, 2007, (4): 219-223.DOI:
夹脊电针对大鼠脊髓损伤后表皮生长因子受体及胶质酸性纤维蛋白表达的影响
摘要
目的:探讨电针治疗大鼠脊髓损伤(SCI)的分子机制。方法:45只成年雄性SD大鼠
随机分为假手术组、模型组和电针组(每组各15只)。采用改良的Allen's法建立SCI模型。电针组采用夹脊电针分别治疗3、7和14 d
Objective: To study the molecular mechanism of electroacupuncture(EA) in the treatment of spinal cord injury(SCI) in rats. Methods: Forty-five male SD rats were randomized into control
model and EA groups with 15 cases in each group which was further divided into 3 subgroups(3 d
7 d and 14 d) at average.SCI(T10) model was duplicated by using modified Allen's method.EA(2 Hz
2-6 mA) was applied to bilateral "Jiaji" [EXB 2
superior and inferior to the injured locus(T10)] for 30 min
continuously for 3 days
7 days and 14 days respectively in different subgroups.Changes of SCI rats' behavior(hind-limb motor) were detected by using Basso Beattie Bresnahan(BBB) locomotor scoring scale.The immuno-reaction(IR) activity of epidermal growth factor receptor(EGFR) and glial fibrillary acidic protein(GFAP) in gray matter of the injured cord was determined using immunohistochemical technique on day 3
7 and 14 separately.Results: Compared with control group
BBB scores of model and EA groups were significantly lower in the 3 subgroups(P<0.01);while in comparison with the 3 subgroups of model group
BBB scores of the corresponding time in EA group were significantly higher(P<0.01).Compared with control group
IR-positive cells of both EGFR and GFAP in model and EA groups increased remarkably at the 3 time-points in number(P<0.01);while those of EGFR and GFAP of EA group were significantly fewer than those of model group at the 3 time-points(P<0.01).Conclusion: EA can effectively improve SCI rats' hind-limb locomotor
which may be closely related to its functions in suppressing the expression of EGFR and GFAP in the injured spinal cord and in promoting nerve axon regeneration.