目的:探讨“嗅三针”电刺激对急性局灶性脑缺血再灌注大鼠神经元线粒体保护作用的机理。方法:SD大鼠30只

分为模型组、电针组和对照组。电针组给予“嗅三针”电刺激

疏密波

频率80-100 Hz

强度1-3 mA

持续60 min。进行线粒体体视学检测

Tietze还原酶法测定血清还原型谷胱甘肽(GSH)

DT-NB直接法测定血清谷胱甘肽过氧化物酶(GSH-Px)。结果:模型组大脑皮层神经元线粒体体视学及血清GSH和GSH-Px检测结果与对照组相比较均有显著性差异(P<0.05);电针治疗后

大脑皮层神经元线粒体体视学及血清GSH和GSH-Px检测结果均有明显改善

与模型组相比较有显著性差异(P<0.05)。结论:“嗅三针”电刺激对急性局灶性脑缺血再灌注大鼠引发的大脑皮层神经元线粒体损伤具有确切的保护作用

其治疗作用可能是通过拮抗活性氧过氧化损伤来实现的。Objective: To explore the protective effect of electroacupuncture(EA) of "Xiusanzhen"(嗅三针) on mitochondria of cortical neurons in acute cerebral ischemia-reperfusion(CI/R) rats.Methods: A total of 30 cases of SD rats were divided into control

model and EA groups.CI/R model was established by occlusion of the middle cerebral artery and reperfusion.EA(80-100 Hz

1-3 mA) was applied to "Xiusanzhen"(the median line of the nose root

2 mm to the lateral median line on the bilateral sides) for 60 min.Content of serum reduced glutathione(GSH) and glutathion peroxidase(GSH-Px) activity were detected by using Tietze's reducase assay and dithio-bis-nitrobenzoic acid(DTNB) direct method respectively.The surface density(Sv)

numerical density(Nv) and perimeter/area(Pv) of the mitochondria of the cerebral neurons were detected using stereological technique and electronic microscope.Results: Compared with control group

Sv and Pv of model group increased significantly(P<0.05)

while serum GSH content and GSH-Px activity of model group decreased considerably(P<0.05).While in comparison with model group

Sv and Pv of EA group lowered obviously(P<0.05)

and GSH and GSH-Px both increased pronouncedly(P<0.05).No significant difference was found among 3 groups in Nv(P>0.05).Conclusion: EA of "Xiusanzhen" has a definite protective effect on mitochondria of cortical neurons in acute focal cerebral ischemia-reperfusion rats

which may be related with its antioxidation.