手针与电针对急性运动大鼠骨骼肌肌浆网Ca~(2+)的影响

高明; 杨华元; 蒯乐; 刘堂义; 顾训杰

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手针与电针对急性运动大鼠骨骼肌肌浆网Ca~(2+)的影响

实验研究 | 更新时间：2023-08-11

- 手针与电针对急性运动大鼠骨骼肌肌浆网Ca~(2+)的影响
- Effects of Manual Acupuncture and Electroacupuncture on Ca~(2+) Content and Ca~(2+)-ATPase Activity in Sarcoplasmic Reticulum of Skeletal Muscle Cells in Rats During Acute Swimming Exercise
- 针刺研究 2008年第1期页码：13-16
- 作者机构：
  
  上海中医药大学中医工程研究室
- 作者简介：
- 基金信息：
  
  上海高校选拔培养优秀青年教师科研专项基金(No.F114)
- DOI：
  中图分类号：
- 纸质出版日期：2008
- 稿件说明：
移动端阅览
高明, 杨华元, 蒯乐, 等. 手针与电针对急性运动大鼠骨骼肌肌浆网Ca~(2+)的影响[J]. 针刺研究, 2008,(1):13-16.

GAO Ming, YANG Hua-yuan, KUAI Le, et al. Effects of Manual Acupuncture and Electroacupuncture on Ca~(2+) Content and Ca~(2+)-ATPase Activity in Sarcoplasmic Reticulum of Skeletal Muscle Cells in Rats During Acute Swimming Exercise[J]. Acupuncture research, 2008, (1): 13-16.
高明, 杨华元, 蒯乐, 等. 手针与电针对急性运动大鼠骨骼肌肌浆网Ca~(2+)的影响[J]. 针刺研究, 2008,(1):13-16. DOI：

GAO Ming, YANG Hua-yuan, KUAI Le, et al. Effects of Manual Acupuncture and Electroacupuncture on Ca~(2+) Content and Ca~(2+)-ATPase Activity in Sarcoplasmic Reticulum of Skeletal Muscle Cells in Rats During Acute Swimming Exercise[J]. Acupuncture research, 2008, (1): 13-16. DOI：

摘要

目的:研究手针和电针对急性游泳运动大鼠骨骼肌肌浆网Ca2+含量、Ca2+-ATP酶活性的影响

探讨针灸增强运动能力的作用机制。方法:60只SD雄性大鼠随机分为空白对照组、模型组、手针组、电针组。手针组和电针组在急性游泳运动前运用针刺和电针两种不同的方法

对"大椎""命门""环跳""足三里"穴进行20 min刺激。其中手针组运用捻转手法

每间隔3

min运针1次(频率约为1205 min运针1次(频率约为120

40次/min)

每次运针30140次/min)

每次运针30

0 s;电针组在针刺后接BX型电针仪

电针参数为音频脉冲调制波

频率为50060 s;电针组在针刺后接BX型电针仪

电针参数为音频脉冲调制波

频率为500

00 Hz

电流为0.20800 Hz

电流为0.20

.25 mA。采用比色法分别测定骨骼肌肌浆网Ca2+含量、Ca2+-ATP酶活性指标。结果:骨骼肌肌浆网Ca2+含量模型组明显低于空白对照组、手针组和电针组(P

0.01);肌浆网Ca2+-ATP酶活性模型组亦明显低于空白对照组、手针组和电针组(P

0.05

0.01)。手针组肌浆网Ca2+-ATP酶活性比空白对照组明显升高(P

0.05);手针组Ca2+含量及Ca2+-ATP酶活性与电针组比较

两组间差异无显著性意义(P

0.05)。结论:针刺可以提高大鼠肌浆网Ca2+-ATP酶活性

增强肌浆网对细胞内Ca2+浓度的调节作用

参与肌肉兴奋收缩偶联并维持肌细胞胞质Ca2+稳态

这是针灸增强运动能力的重要因素之一。

Abstract

Objective To observe the effects of manual acupuncture(MA) and electroacupuncture(EA) on Ca2+ content and Ca2+-ATPase activity in sarcoplasmic reticulum of skeletal muscle cells in rats of acute swimming exercise(ASE) so as to reveal its underlying mechanism in strengthening motor abilities.Methods Sixty male SD rats were randomly divided into control

model

MA and EA groups

with 15 cases in each.Rats in MA group and EA group were treated respectively with MA(120-140 twirlings/min

30-60 s/time every 3-5 min) and EA(500-800 Hz

0.20-0.25 mA) stimulation of "Dazhui"(GV 14)

"Mingmen"(GV 4)

"Huantiao"(GB 30) and "Zusanli"(ST 36) for 20 min before ASE.After ASE(120 min)

the rats anesthetized with urethane(1 g/kg) were killed for sampling skeletal muscles(quadriceps muscle of thigh) and for detecting Ca2+ content and Ca2+-ATPase activity with thymol blue chromatometry and chemical chromatometry respectively.Results In comparison with control group

Ca2+ content and Ca2+-ATPase activity in sarcoplasmic reticulum of skeletal muscle cells in model group were obviously lower(P<0.01

0.05);while compared with model group

both Ca2+ content and Ca2+-ATPase activity in sarcoplasmic reticulum in MA and EA groups increased significantly(P<0.01).The activity of Ca2+-ATPase of MA group was evidently higher than that of control group(P<0.05).No significant differences were found between MA and EA groups in Ca2+ content and Ca2+-ATPase activity(P>0.05).Conclusion Both MA and EA can increase Ca2+-ATPase activity and Ca2+ content in sarcoplasmic reticulum of skeletal muscle cells in motor fatigue rats

which may contribute to their effects in strengthening motor ability.

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