Objective To study the bio-molecule basis of correlativity between Heart Meridian and hypothalamus from gene differential expression. Methods Twenty-eight healthy Sprague-Dawley male rats were divided into 4 groups randomly: normal control

model

Heart Meridian-electroacupuncture (EA) and Lung Meridian-EA groups

with 7 cases in each. Acute myocardial ischemia (AMI) model was duplicated by occlusion of the left anterior descending branch (LAD) of the coronary artery under anesthesia (3% Nemb

40 mg/kg). EA (1.1 mA

2 Hz

duration of waves 300 μs) was applied to bilateral "Shenmen" (HT 7)-"Tongli" (HT 5

Heart Meridian)

"Taiyuan" (LU 9)-"Lieque" (LU 7

Lung Meridian) separately for 20 min

once daily for 3 days. Then

hypothalamus tissue was taken for differentially expressed gene profiling analysis (Affymetrix GeneChip Rat Genome U 230 Affymetrix). Results Compared with model group

among the differentially expressed genes above 2 times in Heart-Meridian-EA group and Lung-Meridian-EA group

190 and 57 were up-regulated

34 and 26 down-regulated separately. Compared with Lung-Meridian-EA group

among the 175 differentially expressed genes above 2 times in hypothalamus tissues of Heart-Meridian-EA group

147 were up-regulated and 28 down-regulated. Conclusion In comparison with EA of Lung Meridian

a different gene expression profile of hypothalamus tissue is involved in the effect of EA of Heart-Meridian on acute myocardial ischemia.