Objective To explore the possible mechanism of scalp acupuncture(SA) in relieving cerebral ischemia reperfusion(CI-R) injury.Methods Seventy male SD rats were randomized into sham-operation(sham

n=10)

model(n=30) and SA(n=30) groups.The later 2 groups were further divided into 24 h

48 h and 72 h subgroups respectively

with 10 cases in each.CI-R model was established by middle cerebral artery occlusion(MCAO) and reperfusion.Electroacupuncture(2 mA

2 Hz/100 Hz) was applied to "Dingnie Houxiexian"(MS 7) and "Dingnie Qianxiexian"(MS 6) for 30 min

once every 24 h.Changes of the animal behavior were observed by using neurological severity score(NSS)

nuclear factor-kappa B(NF-κB) and cyclooxygenase(COX-2) contents and their mRNA expression were detected with enzyme-linked immunosorbent assay(ELISA) and polymerase chain reaction(PCR) techniques respectively.Results Following modeling

the NSS at 24 h

48 h and 72 h after MCAO increased significantly

while compared with model group

NSS of SA group at the 3 time-points decreased considerably(P<0.05

P<0.01)

suggesting an improvement of the neurological functions after SA treatment.In comparison with sham group

NF-κB mRNA and COX-2 mRNA

and NF-κB and COX-2 protein expression of model group in the infarcted cerebral tissue were significantly upregulated at the 3 time-points(P<0.01) except NF-κB mRNA at 72 h(no significant change)

while compared with the 3 time-points of model group

NF-κB mRNA and COX-2 mRNA

and NF-κB and COX-2 protein expression of SA group were downregulated obviously(P<0.01) except COX-2 mRNA at 72 h.Conclusion Scalp acupuncture can suppress cerebral ischemia-induced upregulation of NF-κB mRNA and COX-2 mRNA

and NF-κB and COX-2 protein expression

which may contribute to its effect in promoting neurofunctional rehabilitation of CI-R rats by reducing cytokines-mediated inflammatory reaction.