电针“内关”“天枢”穴对肠易激综合征大鼠结肠动力及结肠D 2受体的影响

王珊; 郭孟玮; 高誉珊; 任晓暄; 蓝莹; 吉毛先; 吴艳英; 李凯歌; 谭莉华; 睢明河

doi:10.13702/j.1000-0607.170416

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电针“内关”“天枢”穴对肠易激综合征大鼠结肠动力及结肠D 2受体的影响

机制探讨 | 更新时间：2023-08-11

- 电针“内关”“天枢”穴对肠易激综合征大鼠结肠动力及结肠D 2受体的影响
- Effect of Electroacupuncture at “Neiguan” (PC 6) and “Tianshu” (ST 25) for Colonic Motility and D2 Receptor in Irritable Bowel Syndrome Rats
- 针刺研究 2018年43卷第1期页码：24-28
- 作者机构：
  
  1. 北京中医药大学针灸推拿学院
  2. 北京中医药大学中医学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金课题(No.81473776)
- DOI：10.13702/j.1000-0607.170416
  中图分类号：
- 纸质出版日期：2018
- 稿件说明：
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王珊, 郭孟玮, 高誉珊, 等. 电针“内关”“天枢”穴对肠易激综合征大鼠结肠动力及结肠D 2受体的影响[J]. 针刺研究, 2018,43(1):24-28.

WANG Shan, GUO Meng-wei, GAO Yu-shan, et al. Effect of Electroacupuncture at “Neiguan” (PC 6) and “Tianshu” (ST 25) for Colonic Motility and D2 Receptor in Irritable Bowel Syndrome Rats[J]. Acupuncture research, 2018, 43(1): 24-28.
王珊, 郭孟玮, 高誉珊, 等. 电针“内关”“天枢”穴对肠易激综合征大鼠结肠动力及结肠D 2受体的影响[J]. 针刺研究, 2018,43(1):24-28. DOI： 10.13702/j.1000-0607.170416.

WANG Shan, GUO Meng-wei, GAO Yu-shan, et al. Effect of Electroacupuncture at “Neiguan” (PC 6) and “Tianshu” (ST 25) for Colonic Motility and D2 Receptor in Irritable Bowel Syndrome Rats[J]. Acupuncture research, 2018, 43(1): 24-28. DOI： 10.13702/j.1000-0607.170416.

摘要

目的:观察并比较电针"天枢""内关"穴对肠易激综合征(IBS)大鼠结肠动力及结肠多巴胺D 2受体表达的影响

探讨不同经脉和不同穴性穴位的效应特异性。方法:Wistar新生大鼠随机分为空白对照组、模型对照组、天枢组和内关组

每组10只。采用母子分离与醋酸灌肠再结合结直肠扩张的方法制备IBS大鼠模型。天枢组和内关组分别给予双侧"天枢"和"内关"电针干预

隔日治疗1次

共5次。记录大鼠Bristol粪便评分、腹部回撤反射的第1次收缩波潜伏期和90s内收缩波个数及免疫组化法检测结肠多巴胺D 2受体的表达。结果:与空白对照组相比

模型对照组大鼠的Bristol评分显著增高(P<0.01)

第1次收缩波潜伏期明显缩短(P<0.01)

90s内收缩波个数明显增多(P<0.01)

结肠内D 2受体免疫反应物阳性表达水平明显下降(P<0.01)。与模型对照组相比

天枢组和内关组大鼠治疗后的Bristol评分均显著下降(P<0.01)

第1次收缩波潜伏期均明显延长(P<0.01)

90s内收缩波个数明显减少(P<0.01)

结肠内D 2受体免疫反应物阳性表达水平明显增强(P<0.01

P<0.05)。与天枢组相比

内关组结肠内D 2受体免疫反应物阳性表达水平下降(P<0.01)。结论:电针"天枢""内关"穴均可明显改善IBS大鼠的结肠动力异常症状

但电针"天枢"穴的效应更佳

说明不同经脉和穴性的穴位存在效应特异性。

Abstract

Objective To observe and compare the effects of electroacupuncture(EA)at"Tianshu"(ST 25)and"Neiguan"(PC 6)for colonic motility and the expression of colon dopamine D 2 in irritable bowel syndrome(IBS)rats

and to explore the specificity of different meridians and different acupoints.Methods Forty Wistar newborn rats were randomly divided into blank

model

Tianshu and Neiguan groups.Separation of mother and child and acetic acid coloclyster combined with colorectal distension were used to establish IBS model in the model

Tianshu and Neiguan groups.At the age of 9 weeks

EA at bilateral ST 25 and PC 6 were applied in the corresponding groups 5 times

once every other day.After the intervention

the Bristol fecal score

the latent period of abdominal retraction reflex and the number of contraction waves were recorded.The expression of dopamine D 2 receptor was detected by immunohistochemistry.Results Compared with the blank group

the Bristol fecal score of the model group was higher(P<0.01)

the 1 st contraction wave latent period was shorter(P<0.01)

the number of contraction waves in 90 sincreased(P<0.01)

the immunoreactive expression of D 2 receptor in colon decreased(P<0.01).Compared with the model group

the Bristol fecal scores of the Tianshu and Neiguan groups decreased(P<0.01)

the 1 st contraction wave latent periods were longer(P<0.01)

the numbers of contraction waves in 90 sdecreased(P<0.01)

the positive expressions of D 2 receptor in colon increased(P<0.01

P<0.05).Compared with the Tianshu group

the immunoreactive expression of D 2 receptor in the Neiguan group decreased(P<0.01).Conclusion EA at ST 25 and PC 6 can improve the symptoms of colonic motility in IBS rats.The effect of EA at ST 25 is better

which indicates that different meridians and different acupoints play specific effects.

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