Since the invention of optogenetic technology

it has greatly promoted the development of neuroscience.Currently

optogenetic approaches have been mostly used to map neural circuits and new neuropharmacology but are rarely seen in the research field of acupuncture analgesia.The mechanism of neural circuits contributing to acupuncture analgesia

an important research hotspot in recent years

has not been fully determined.The optogenetic techniques can be used to modulate and control specific cells

provides highly precise spatial and temporal resolution

is repeatable

and may functionally dissect neuronal networks in vivo.The neuronal activities and their information transmission

processing and storage in intercluster neural networks in different brain regions

and the correlation between behavioral changes and electrical activities of neurons in vivo studies are mainly captured by the implanted microelectropode array

etc.If these two(or more)approaches are combined together

it is definitely and highly helpful to reveal the driving dynamics of neural circuits

plasticity and temporal-spatial activity mode of neurons

as well as behavioral reactions of animals with chronic pain during acupuncture analgesia and may open a new prospect for the application of acupuncture analgesia study.