电针“神门”“三阴交”对失眠大鼠下丘脑室旁核能量代谢的影响

诸毅晖; 杨翠花; 何林熹; 乔玲; 成词松

doi:10.13702/j.1000-0607.170996

您当前的位置：

首页 >

文章列表页 >

电针“神门”“三阴交”对失眠大鼠下丘脑室旁核能量代谢的影响

机制探讨 | 更新时间：2023-08-11

- 电针“神门”“三阴交”对失眠大鼠下丘脑室旁核能量代谢的影响
- Effect of electroacupuncture at “Shenmen”(HT7) and “Sanyinjiao”(SP6) on energy metabolism in paraventricular nucleus of hypothalamus of insomnia rats
- 针刺研究 2019年44卷第3期页码：170-175
- 作者机构：
  
  1. 成都中医药大学针灸推拿学院
  2. 成都中医药大学基础医学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(No.81273851)
- DOI：10.13702/j.1000-0607.170996
  中图分类号：
- 纸质出版日期：2019
- 稿件说明：
移动端阅览
诸毅晖, 杨翠花, 何林熹, 等. 电针“神门”“三阴交”对失眠大鼠下丘脑室旁核能量代谢的影响[J]. 针刺研究, 2019,44(3):170-175.

ZHU Yi-hui, YANG Cui-hua, HE Lin-xi, et al. Effect of electroacupuncture at “Shenmen”(HT7) and “Sanyinjiao”(SP6) on energy metabolism in paraventricular nucleus of hypothalamus of insomnia rats[J]. Acupuncture research, 2019, 44(3): 170-175.
诸毅晖, 杨翠花, 何林熹, 等. 电针“神门”“三阴交”对失眠大鼠下丘脑室旁核能量代谢的影响[J]. 针刺研究, 2019,44(3):170-175. DOI： 10.13702/j.1000-0607.170996.

ZHU Yi-hui, YANG Cui-hua, HE Lin-xi, et al. Effect of electroacupuncture at “Shenmen”(HT7) and “Sanyinjiao”(SP6) on energy metabolism in paraventricular nucleus of hypothalamus of insomnia rats[J]. Acupuncture research, 2019, 44(3): 170-175. DOI： 10.13702/j.1000-0607.170996.

摘要

目的:观察电针"神门""三阴交"对失眠大鼠下丘脑室旁核(PVN)能量代谢的影响

探讨电针治疗失眠症的中枢调节机制。方法:SD大鼠随机分为正常组、模型组和电针组

每组22只。模型组和电针组采用慢性束缚法束缚15d复制失眠模型。电针组电针"神门""三阴交"

每日1次

每次15min

治疗5d。用24h小动物自发活动数据采集系统检测大鼠24h自发活动量

以负重力竭游泳实验检测大鼠负重力竭游泳时间

采用蛋白免疫印迹法检测PVN内的腺苷酸活化蛋白激酶(AMPK)表达

酶联免疫法检测PVN内的乙酰辅酶A(Ac-CoA)、钠-钾三磷酸腺苷酶(Na+-K+-ATPase)含量以及血浆皮质酮(CORT)含量

透射电镜观察PVN的细胞结构。结果:与正常组比较

模型组大鼠白天活动量增多、夜晚活动量减少(P

0.05)

负重力竭游泳时间缩短(P

0.05);PVN内AMPK表达增强(P

0.05)、Ac-CoA和Na

-K+-K

-ATPase含量降低(P

0.05)

血浆CORT含量降低(P

0.05);电镜下可见模型大鼠PVN内线粒体肿胀

核糖体部分消失。电针后

与模型组比较

电针组大鼠白天活动量减少、夜晚活动量增多(P

0.05)

负重力竭游泳时间延长(P

0.05);PVN内AMPK表达减弱(P

0.05)、Ac-CoA和NA+-ATPase含量降低(P

0.05)

血浆CORT含量降低(P

0.05);电镜下可见模型大鼠PVN内线粒体肿胀

核糖体部分消失。电针后

与模型组比较

电针组大鼠白天活动量减少、夜晚活动量增多(P

0.05)

负重力竭游泳时间延长(P

0.05);PVN内AMPK表达减弱(P

0.05)、Ac-CoA和NA

-K+-K

-ATPase含量升高(P

0.05)

血浆CORT含量升高(P

0.05);PVN内线粒体仅轻度肿胀。结论:电针"神门""三阴交"对失眠大鼠下丘脑室旁核能量代谢有良好调整作用

通过调节下丘脑室旁核AMPK表达、Ac-CoA和Na+-ATPase含量升高(P

0.05)

血浆CORT含量升高(P

0.05);PVN内线粒体仅轻度肿胀。结论:电针"神门""三阴交"对失眠大鼠下丘脑室旁核能量代谢有良好调整作用

通过调节下丘脑室旁核AMPK表达、Ac-CoA和Na

-K+-K

-ATPase含量

电针可以有效改善失眠及失眠后疲劳症状。Objective To observe the effect of electroacupuncture(EA)at"Shenmen"(HT7)and"Sanyinjiao"(SP6)on energy metabolism in paraventricular nucleus(PVN)of hypothalamus in insomnia rats

so as to explore its mechanism underlying improvement of insomnia.Methods A total of 66 SD rats(half male and half female)were randomized into 3 groups:normal control

model and EA groups(n=22 per group).The insomnia model was established by binding the rat for at least 4 h(step increase of 30 min per day)

once daily for 15 days.EA(5 Hz/25 Hz

0.5-1.0 mA)was applied to unilateral HT7 and SP6 for 15 min

once daily for 5 days.The rats' spontaneous activities during day and night were recorded by using the ClockLab Data Collection and Analysis System

and the duration of exhausted swimming was detected by using load-bearing endurance swimming test.The expression of adenosine 5′-monophosphate(AMP)-activated protein kinase(AMPK)of PVN tissue was assayed by Western blot

and the contents of acetyl coenzyme A(Ac-CoA)and Na+-ATPase含量

电针可以有效改善失眠及失眠后疲劳症状。

Abstract

Objective To observe the effect of electroacupuncture(EA)at"Shenmen"(HT7)and"Sanyinjiao"(SP6)on energy metabolism in paraventricular nucleus(PVN)of hypothalamus in insomnia rats

so as to explore its mechanism underlying improvement of insomnia.Methods A total of 66 SD rats(half male and half female)were randomized into 3 groups:normal control

model and EA groups(n=22 per group).The insomnia model was established by binding the rat for at least 4 h(step increase of 30 min per day)

once daily for 15 days.EA(5 Hz/25 Hz

0.5-1.0 mA)was applied to unilateral HT7 and SP6 for 15 min

once daily for 5 days.The rats' spontaneous activities during day and night were recorded by using the ClockLab Data Collection and Analysis System

and the contents of acetyl coenzyme A(Ac-CoA)and Na

-K+-K

adenosine triphosphatase(Na+-K+-ATPase)in the PVN tissue

and corticosterone(CORT)in plasma were assayed by ELISA.Changes of the ultrastructure of PVN cells were observed by transmission electron microscope.Results After modeling

the rats' daytime and nocturnal locomotor activities were significantly increased and decreased

respectively(P

0.05)

and the duration of exhausted swimming was considerably shortened in the model group compared with that of the normal control group(P

0.05).The expression level of AMPK protein in the PVN was obviously up-regulated(P

0.05)

and the contents of Ac-CoA and Na+adenosine triphosphatase(Na+-K+-ATPase)in the PVN tissue

and corticosterone(CORT)in plasma were assayed by ELISA.Changes of the ultrastructure of PVN cells were observed by transmission electron microscope.Results After modeling

the rats' daytime and nocturnal locomotor activities were significantly increased and decreased

respectively(P

0.05)

and the duration of exhausted swimming was considerably shortened in the model group compared with that of the normal control group(P

0.05).The expression level of AMPK protein in the PVN was obviously up-regulated(P

0.05)

and the contents of Ac-CoA and Na

-K+-K

-ATPase in PVN and CORT in plasma were markedly decreased in the model control group relevant to the normal group(P

0.05).After EA intervention

the increased daytime locomotion and the decreased nocturnal activities

the shortened duration of exhausted swimming

the up-regulated expression of AMPK

and the decreased Ac-CoA

Na+-K+-ATPase and CORT contents were all reversed in the EA treated rats relevant to those of the insomnia rats(all P

0.05).Moreover

ultrastructural observation showed mitochondrial swelling and disappearance of partial ribosomes in the plasma of PVN cells in the model group

while in the EA group

only mild swelling of some mitochondria was found

being with basically normal nuclear membrane

mitochondria

rough endoplasmic reticulum

Golgi complex and ribosomes.Conclusion EA at HT7 and SP6 has a positive effect in improving insomnia and insomnia-induced fatigue in insomnia rats

which may be associated with its effects in restraining the expression of AMPK protein

and up-regulating the contents of Ac-CoA and Na+-ATPase in PVN and CORT in plasma were markedly decreased in the model control group relevant to the normal group(P

0.05).After EA intervention

the increased daytime locomotion and the decreased nocturnal activities

the shortened duration of exhausted swimming

the up-regulated expression of AMPK

and the decreased Ac-CoA

Na+-K+-ATPase and CORT contents were all reversed in the EA treated rats relevant to those of the insomnia rats(all P

0.05).Moreover

ultrastructural observation showed mitochondrial swelling and disappearance of partial ribosomes in the plasma of PVN cells in the model group

while in the EA group

only mild swelling of some mitochondria was found

being with basically normal nuclear membrane

mitochondria

rough endoplasmic reticulum

Golgi complex and ribosomes.Conclusion EA at HT7 and SP6 has a positive effect in improving insomnia and insomnia-induced fatigue in insomnia rats

which may be associated with its effects in restraining the expression of AMPK protein

and up-regulating the contents of Ac-CoA and Na

-K+-K

-ATPase in PVN and CORT in plasma.

关键词

Keywords

references

浏览量

895

下载量

CNKI被引量

文章被引用时，请邮件提醒。

提交

工具集

关联资源

杏仁中央核-下丘脑室旁核神经环路介导胃俞募配穴针刺调节胃功能机制研究

电针五脏俞对失眠大鼠脑内神经递质及细胞因子的调节作用

电针单穴与腧穴配伍对失眠大鼠血清中白介素-1β、脑源性神经生长因子等睡眠-觉醒相关因子含量的影响

针药结合对脑缺血大鼠下丘脑室旁核脑源性神经营养因子和血管内皮生长因子表达的影响