Laser scanning confocal microscope(LSCM)has been used in the field of morphological research of acupuncture and moxibustion for more than 20 years.It is one of the important tools for revealing the structure of acupoints and the effect of acupuncture from histological perspective.With the help of technical advantages of LSCM

the quality of morphological research of acupuncture and moxibustion has been greatly improved

helping us gain a deeper understanding about the structure of acupoints and meridians as well as histochemical changes induced by acupuncture/moxibustion intervention.In order to promote the application of LSCM in acupuncture and moxibustion

we simply reviewed some recent studies in this field and combined them with our experience

trying to provide some technical suggestions.We expect that the technique of LSCM could be integrated into more experiments in acupuncture medicine to provide more powerful morphological evidence for exploring the underlying mechanisms of acupuncture and moxibution therapies.