ObjectiveTo observe the effect of electroacupuncture(EA) of"Baihui"(GV20) and"Zusanli"(ST36)on the expression of stromal cell derived factor-1α(SDF-1α)and CD34 in ischemic cortex tissue of cerebral ischemia/reperfusion injury(CI/RI)rats

so as to study its mechanisms underlying improving CI/RI.MethodsThirty male SD rats were equally and randomly divided into sham operation

model and EA groups(n=10 rats in each group). The CI/RI model was established by occlusion of the middle cerebral artery for 120 min

followed by reperfusion. EA(40 Hz

1-2 mA)was applied to GV20 and left ST36 for 20 min

once daily for successive 14 days. The neurological deficit severity was assessed by using Longa's and colleagues' methods. The histopathological changes of the ischemic tissues were observed after H. E. staining and the expression of SDF-1α and CD34 in the ischemic cortex tissues was detected by immunohistochemistry.ResultsAfter modeling

the neurological deficit score

and the numbers of SDF-1α and CD34-positive cells in the ischemic cerebral cortex tissue were significantly increased in the model group(P<0. 05). Following EA intervention

the increased neurological deficit score was reversed at the 3 rd

7 thand 14 thday

and the increased SDF-1α and CD34-positive cells were significantly further up-regulated in the EA group(P<0. 05). H. E. staining showed tissue edema

widening of the intercellular space

cavitation-like changes

neuronal shrinkage

nuclear pyknosis with hyperchroma or even disappearance

and aggregation of inflammatory and neurogliocytes in the model group.These situations were relatively milder in the EA group.ConclusionEA of GV20 and ST36 can improve neurological function of CI/RI rats

which may be associated with its effect in up-regulating the expression of SDF-1α and CD34 proteins in the ischemic cerebral cortex tissues.