电针“百会”“涌泉”对APP/PS1双转基因小鼠海马突触可塑性相关蛋白表达的影响

杨光; 裴亚妮; 邵淑君; 高誉珊; 张淑静; 胡聪; 冯思凝; 薛卫国

doi:10.13702/j.1000-0607.190012

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电针“百会”“涌泉”对APP/PS1双转基因小鼠海马突触可塑性相关蛋白表达的影响

机制探讨 | 更新时间：2023-08-11

- 电针“百会”“涌泉”对APP/PS1双转基因小鼠海马突触可塑性相关蛋白表达的影响
- Effects of electroacupuncture at “Baihui” and “Yongquan” on the levels of synaptic plasticity related proteins postsynaptic density-95 and synaptophysin in hippocampus of APP/PS1 mice
- 针刺研究 2020年45卷第4期页码：310-314
- 作者机构：
  
  1. 北京中医药大学针灸推拿学院
  2. 晋城市人民医院中医科
  3. 北京中医药大学中医学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(No.81273826);北京中医药大学研究生自主课题(No.2017-JYB-XS)
- DOI：10.13702/j.1000-0607.190012
  中图分类号：
- 纸质出版日期：2020
- 稿件说明：
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杨光, 裴亚妮, 邵淑君, 等. 电针“百会”“涌泉”对APP/PS1双转基因小鼠海马突触可塑性相关蛋白表达的影响[J]. 针刺研究, 2020,45(4):310-314.

YANG Guang, PEI Ya-ni, SHAO Shu-jun, et al. Effects of electroacupuncture at “Baihui” and “Yongquan” on the levels of synaptic plasticity related proteins postsynaptic density-95 and synaptophysin in hippocampus of APP/PS1 mice[J]. Acupuncture research, 2020, 45(4): 310-314.
杨光, 裴亚妮, 邵淑君, 等. 电针“百会”“涌泉”对APP/PS1双转基因小鼠海马突触可塑性相关蛋白表达的影响[J]. 针刺研究, 2020,45(4):310-314. DOI： 10.13702/j.1000-0607.190012.

YANG Guang, PEI Ya-ni, SHAO Shu-jun, et al. Effects of electroacupuncture at “Baihui” and “Yongquan” on the levels of synaptic plasticity related proteins postsynaptic density-95 and synaptophysin in hippocampus of APP/PS1 mice[J]. Acupuncture research, 2020, 45(4): 310-314. DOI： 10.13702/j.1000-0607.190012.

摘要

目的:从β-淀粉样蛋白(Aβ)对突触的毒性作用和突触可塑性的角度探讨电针在阿尔茨海默病病理前期改善学习记忆能力的机制。方法:将12只4月龄雄性APP/PS1转基因小鼠随机分为电针组和模型组

每组6只;以6只4月龄雄性C57BL/6小鼠作为正常组。电针组针刺"百会""涌泉"穴

双侧"涌泉"穴接电针

每次电针15 min

每周3次

连续6周。采用免疫荧光法观察小鼠左侧海马Aβ阳性表达水平

免疫组织化学法观察小鼠左侧海马突触后致密物-95(PSD-95)的阳性表达

Western blot法检测小鼠右侧海马PSD-95和突触素(SYN)的蛋白表达水平。结果:免疫荧光结果显示

模型组和电针组海马区可见细胞外的Aβ

但未见团状老年斑;与正常组比较

模型组海马区Aβ表达水平显著增加(P<0.01);与模型组比较

电针组海马区Aβ表达水平减少(P<0.05)。免疫组织化学结果显示

与正常组比较

模型组左侧海马区PSD-95阳性表达减少(P<0.05);与模型组比较

电针组左侧海马区PSD-95阳性表达增加(P<0.05)。Western blot结果显示

与正常组比较

模型组右侧海马区PSD-95、SYN的表达水平降低(P<0.05

P<0.01);与模型组比较

电针组右侧海马区PSD-95、SYN的表达水平增高(P<0.05

P<0.01)。结论:电针可以降低APP/PS1小鼠海马内Aβ的表达水平、升高PSD-95和SYN表达水平

提示电针可能通过降低海马内Aβ表达

减少Aβ对突触的毒性作用

从而改善其突触可塑性。

Abstract

Objective From the perspective of β-amyloid(Aβ) toxicity and synaptic plasticity

the mechanism of electroacupuncture to improve learning and memory ability in the early pathological stages of Alzheimer's disease was explored. Methods Twelve male amyloid-protein precursor(APP)/γ-secretase(PS1) double transgenic AD mice were randomly and equally divided into electroacupuncture(EA) group and model group

and other 6 male C57 BL/6 mice were used as the normal group. EA(1 Hz/50 Hz

0.5 mA) was applied to "Baihui"(GV20) and bilateral "Yongquan"(KI1) for 15 min

once every other day for 6 weeks. Immunofluorescence was used to observe the positive expression of Aβ in the left hippocampus. Immunohistochemistry was used to observe the positive expression of postsynaptic density-95(PSD-95) in the left hippocampus. Western blot was used to detect the expression of PSD-95 and synaptophysin(SYN)in the right hippocampus. Results Immunofluorescence results showed that extracellular Aβ was seen in the model group and electroacupuncture group

but no senile plaques were seen. Compared with the normal group

the expression level of Aβ in the hippocampus of the model group increased significantly(P<0.01). Compared with the model group

the expression of Aβ in the hippocampus of the EA group decreased(P<0.05). Immunohistochemical results showed that compared with the normal group

the PSD-95 positive expression in the model group was decreased(P<0.05). Compared with the model group

the expression of PSD-95 in the EA group was increased(P<0.05). Western blot results showed that compared with the normal group

the expression levels of PSD-95 and SYN in the hippocampus of the model group were decreased(P<0.05

P<0.01). Compared with the model group

the expression levels of PSD-95 and SYN in the EA group were increased(P<0.05

P <0.01). Conclusion EA can reduce the expression of Aβ in the hippocampus of APP/PS1 mice and increase the expression of PSD-95 and SYN

which may contribute to its effect in improving the synaptic plasticity.

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