Objective To observe the effect of electroacupuncture(EA) pretreatment at "Neiguan"(PC6) on expression profiles of myocardial long non-coding RNAs(LncRNAs) and mRNAs in myocardial ischemia-reperfusion injury(MIRI) mice

so as to explore its mechanisms underlying prevention of MIRI via regulating LncRNA expression.Methods C57 BL/6 mice were randomly divided into sham

model

and EA groups(n =4 in each group). The MIRI model was established by occlusion of the anterior descending branch(ADB) of the left coronary artery for 30 min

followed by reperfusion for 24 h. In the sham group

the ADB was only threaded beneath the artery without ligation. EA was applied to bilateral PC6 for 30 min prior to ischemia induction. Surgery was performed within 30 min at the end of EA stimulation. The expression profiles of differentially expressed LncRNAs and mRNAs in the left ventricular myocardium were analyzed by using LncRNA microarray. Results There was a significant diffe-rence in the expression pattern of LncRNAs and mRNAs among the sham

model and EA groups. A total of 1 693 LncRNAs and 2 858 mRNAs between the model and sham groups

and 3 859 LncRNAs and 1 343 mRNAs between the EA and model groups were identified to be differentially expressed candidates. According to Venn intersection analysis

LncRNAs with opposite regulative orientations in the model and EA groups were screened and defined as EA-related LncRNAs. LncRNA-mRNA co-expression analysis and Gene Ontology enrichment analysis of the EA-related LncRNAs predicted their roles to regulating post-traumatic stress and repairing of myocardial cells. Meanwhile

the proteins' function encoded by EA-related mRNAs mainly involved post-traumatic stress and inflammatory regulation.Conclusion EA pretreatment at PC6 acupoint can produce extensive regulation on myocardial LncRNAs and mRNAs in MIRI mice

suggesting an involvement of LncRNAs in EA pretreatment induced improvement of MIRI. These results may provide direction and molecular basis for subsequent in-depth studies to reveal the underlying mechanisms of EA pretreatment for MIRI.