Objective To observe the effect of moxibustion on the growth of tumor and expression of fibroblast growth factor receptor 1(FGFR1) and vascular endothelial cell growth factor receptor 2(VEGFR2) in mice with sarcoma

so as to explore its mechanisms underlying inhibiting sarcoma growth.Methods C57 BL/6 J mice(half male and half female) were inoculated with S180 sarcoma cells to form transplanted tumors

and divided into model control

medication and moxibustion groups

with 10 mice in each group. Moxibustion was applied to the transplanted tumor directly for 10 min

once a day for 14 days. After the treatment

Luminex liquid suspension chip was used to detect the contents of serum vascular endothelial growth factor(VEGF)

FGFR1 and VEGFR2. The weight of the transplanted tumor was measured

and the expression of VEGF in the transplanted tumor was detected by immunohistochemistry

and the expression of FGFR1 and VEGFR2 mRNAs in the transplanted tumor was detected by fluorescence in situ hybridization.Results The tumor weight

VEGF immunoactivity

serum VEGF

VEGFR2 and FGFR1 contents

and expression levels of VEGFR2 and FGFR1 mRNAs in the transplanted tumor were significantly lower in the moxibustion group than in the model group(P<0.001

P<0.01

P<0.05). Compared with the model group

the tumor weight was remarkably lower in the medication group(P<0.001). Compared with the medication group

th VEGF immunoactivity and the contents of serum VEGF

VEGFR2 and FGFR1 were significantly lower in the moxibustion group(P<0.01

P<0.05). H.E. staining showed a large number of red blood cells were observed in the microenvironment of the transplanted tumor in the moxibustion group rather than in the medication group.Conclusion Moxibustion can inhibit the growth of tumor in mice with sarcoma

which may be related to its function in reducing the expression of FGFR1 and VEGFR2 to inhibit angiogenesis.