Objective To observe the effect of application at Back-Shu with Front-Mu acupoints on serum uric acid(SUA) and kidney uric acid transport related proteins in hyperuricemia rats

so as to explore the mechanism of Shu-Mu acupoint application on treatment of hyperuricemia. Methods SD rats were randomly divided into blank control

model

vaseline application and medication application groups

with 8 rats in each group. The hyperuricemia rat model was established by gavage of potassium oxonate. Rats in the vaseline application group received application of vaseline at bilateral "Ganshu"(BL18) and "Qimen"(LI14)

"Pishu"(BL20) and "Zhangmen"(LR13)

"Shenshu"(BL23) and "Jingmen"(GB25). Rats in the medication application group received application of traditional Chinese medicine at the same acupoints. The contents of SUA and creatinine(SCr) were detected by automatic biochemical analyzer. H.E. staining was used to observe the pathological changes of kidney. And the protein expression levels of organic anion transporter 1(OAT1) and adenosine triphosphate binding cassette transporter G2(ABCG2) were detected by immunohistochemistry. Results Rats in the model group showed symptoms such as polydipsia

polyuria

loose stools

fatigue

weakness

etc. The renal tubules atrophied

and urate crystals can be seen in the lumen. Compared with the control group. the SUA content in the model group increased(P<0.01)and the expressions of OAT1 and ABCG2 protein in kidney decreased(P<0.01). After intervention and in comparison with the model group showed that

the diet

excretion function

and mental state of the rats in the medication application group returned to normal

and the pathological changes of the kidney tissue were alleviated

the SUA content was down-regulated(P<0.01)and the expression levels of OAT1 and ABCG2 in the kidney up-regulated(P<0.01). There was no statistically significant difference in the SCr content among the 4 groups(P>0.05). Conclusion Medication application at Shu-Mu points can effectively reduce the SUA level of hyperuricemia rats

which may be related to its effects in up-regulating the protein expressions of OAT1 and ABCG2 in the kidney and reducing the damage to the kidneys.