Objective To compare the effects of electroacupuncture (EA) of Shu-acupoint "Weishu" (BL 21)

Mu-acupoint "Zhongwan" (CV 12) and BL 21+CV 12 on serum large molecular metabolites in functional dyspepsia (FD) rats. Methods A t otal of 50 Wistar rats were randomly divided into control

model

BL 21

CV 12 and BL 21+CV 12 groups (n=10/group). FD model was established by clamping the rats' tails for 30 min

once every 3 h

4 times daily for 7 days. EA was applied to BL 21

CV 12

or BL 21+CV 12

respectively for 20 min

once daily for 10 days. The rats' venous blood was collected for detecting large molecular metabolites by 1H nuclear magnetic resonance (NMR) spectrometer (Varian UnityInova

600 MHz). Segmental integral calculus (0.04 ppm per segment) was performed from 6.0-0 ppm (longitudinal eddy-delay

LED) as defaulted 1H spectra values by using the VNMR software. Data was saved as text or excel files after normalization and then used for pattern recognition analyses. All the data were analyzed by principal component analysis (PCA) using the SIMCA-P 10.04 software (Umetrics AB

Umea

Sweden)

and orthogonal signal correction and partial least squares discriminant analysis (OSC-PLS-DA). Results The rat serum proton-LED-1H NMR spectra revealed different metabolic spectra among control

model

BL 21

CV 12

and BL 21+CV 12 groups.PCA and OSC-PLS-DA showed that compared with the normal control group

FD rats in model group displayed serum N-acetylglucoprotein (NAc) content was reduced considerably

high density lipoprotein (HDL) level decreased to a certain degree

while very low density lipoprotein (VLDL) / LDL increased significantly.In comparison with model group

VLDL/LDL was decreased in BL 21

CV 12 and BL 21+CV 12 groups

while NAc content increased. Compared with BL 21 and CV 12 groups respectively

HDL level in BL 21+CV 12 increased to a certain degree. Conclusion EA of BL 21

CV 12 and BL 21+CV 12 groups can reverse or relieve FD induced increase of VLDL/LDL and decrease of serum NAc level in rats

which may contribute to its function in improving symptoms of FD patients.