电针对淀粉样前体蛋白转基因小鼠海马微血管淀粉样沉积的影响及其与低密度脂蛋白相关受体1的关系

薛卫国; 张忠; 许红; 吴海霞; 白丽敏

doi:10.13702/j.1000-0607.2011.02.005

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电针对淀粉样前体蛋白转基因小鼠海马微血管淀粉样沉积的影响及其与低密度脂蛋白相关受体1的关系

实验研究 | 更新时间：2023-08-11

- 电针对淀粉样前体蛋白转基因小鼠海马微血管淀粉样沉积的影响及其与低密度脂蛋白相关受体1的关系
- Effect of Electroacupuncture on Learning-memory Ability,and Aβ and LRP1 Immunoactivity in Hippocampal Sulcus Microvessels in APP Transgenic Mice
- 针刺研究 2011年36卷第2期页码：95-100
- 作者机构：
  
  北京中医药大学基础医学院人体形态系
- 作者简介：
- 基金信息：
  
  高等学校博士学科点专项科研基金资助项目(No.20060026015)
- DOI：10.13702/j.1000-0607.2011.02.005
  中图分类号：
- 纸质出版日期：2011
- 稿件说明：
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薛卫国, 张忠, 许红, 等. 电针对淀粉样前体蛋白转基因小鼠海马微血管淀粉样沉积的影响及其与低密度脂蛋白相关受体1的关系[J]. 针刺研究, 2011,36(2):95-100.

XUE Wei-guo, ZHANG Zhong, XU Hong, et al. Effect of Electroacupuncture on Learning-memory Ability,and Aβ and LRP1 Immunoactivity in Hippocampal Sulcus Microvessels in APP Transgenic Mice[J]. Acupuncture research, 2011, 36(2): 95-100.
薛卫国, 张忠, 许红, 等. 电针对淀粉样前体蛋白转基因小鼠海马微血管淀粉样沉积的影响及其与低密度脂蛋白相关受体1的关系[J]. 针刺研究, 2011,36(2):95-100. DOI： 10.13702/j.1000-0607.2011.02.005.

XUE Wei-guo, ZHANG Zhong, XU Hong, et al. Effect of Electroacupuncture on Learning-memory Ability,and Aβ and LRP1 Immunoactivity in Hippocampal Sulcus Microvessels in APP Transgenic Mice[J]. Acupuncture research, 2011, 36(2): 95-100. DOI： 10.13702/j.1000-0607.2011.02.005.

摘要

目的:探讨电针治疗阿尔茨海默病的可能作用机制。方法:将13月龄的淀粉样前体蛋白(APP)转基因小鼠随机分为模型组和电针治疗组

以相同月龄和背景的C 57 BL/6小鼠作对照组。电针治疗组取"百会""涌泉"穴

每次留针15 min

隔日1次

针刺3个月。以LashleyⅢ水迷宫测定各组小鼠的学习记忆能力

以免疫组化方法检测海马β淀粉样蛋白(amyloidβprotein

Aβ)、低密度脂蛋白相关受体1(low densi-ty lipoprotein receptor-related protein 1

LRP 1)的表达。结果:模型组水迷宫游出时间较对照组延长(P<0.05)

海马沟微血管壁Aβ1-42表达的累积吸光度较对照组升高(P<0.01)

海马沟微血管壁LRP 1表达较对照组减低(P<0.01);而电针治疗组的水迷宫游出时间明显低于模型组

海马沟微血管壁Aβ1-42表达低于模型组

海马沟微血管壁LRP 1表达高于模型组(均P<0.05)。结论:电针治疗可能通过提高脑微血管壁Aβ受体LRP 1的清除转运能力

降低APP转基因鼠脑微血管壁的Aβ沉积

从而改善其学习、记忆能力。

Abstract

Objective To investigate the effect of electroacupuncture(EA) of "Baihui"(GV20) and "Yongquan"(KI1) on the expression of amyloid β protein(Aβ) and low density lipoprotein(LDL) receptor-related protein 1(LRP1) in the hippo-campal sulcus microvessels in amyloid precursor protein(APP) 695V717Ⅰ transgenic mice

so as to study its mechanism underlying relief of Alzheimer Disease(AD).Methods Twelve APP 695V717Ⅰ transgenic mice were randomly divided into model group and EA group.Six C 57 BL/6 mice were used as the control group.EA(2Hz/100Hz

3-5mA) was applied to "Baihui"(GV20) and "Yongquan"(KI1) for 15min

once every other day for three months.The learning-memory ability of mice was detected by using Lashley Ⅲ water maze system.The expression level of Aβ1-42

and LRP1 in the hippocampal sulcus microvessels were measured by immunohistochemistry.Results Water maze test showed that the swimming duration from the start to the goal box(terminal) in the Lashley Ⅲ water maze was significantly longer in the model group than in the control group(P<0.05)

suggesting a markedly lower learning-memory capacity of APP 695V717Ⅰ transgenic mice.Compared with the model group

the swimming duration in the EA group was decreased considerably(P<0.05).The integrated optical density(IOD) value of hippo-campal Aβ1-42 immunoreaction(IR) positive products in the model group was significantly higher than that in the control group(P<0.01) and the IOD value of hippocampal LRP 1 IR-positive products in the model group was apparently lo-wer than that in the control group(P<0.01).In comparison with the model group

the IOD value of Aβ 1-42 IR-positive products in the EA group was obviously lower than that in the model group(P<0.05)

while that of LRP1 IR-positive products in the EA group was significantly higher than that in the model group(P<0.05)

suggesting down-regulation of hippocampal Aβ1-42 expression and up-regulation of LRP1 expression after EA

and reduction of deposition of Aβ in the cerebral microvessels after EA.Conclusion EA can improve the learning-memory capacity of APP transgenic mice

which is closely related to its effects in up-regulating hippocampal LRP1 expression and down-regulating hippocampal Aβ1-42 expression.

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