Objective To observe the effect of transcutaneous electrical acupoint stimulation(TEAS) combined with general anesthesia on gastric dynamics in controlled hypotension dogs

so as to provide experimental evidence for compound acupuncture anesthesia.Methods Eighteen male beagle dogs were randomly divided into general anesthesia group(GA group

n=6)

general anesthesia + controlled hypotension group(GA+CHT group

n=6) and general anesthesia combined with TEAS + controlled hypotension group(TEAS group

n=6).The latter two groups were administered with the same anesthetics [iso-fluane inhalation and intravenous sodium nitroprusside(SNP)]for inducing controlled hypotension(being the 40% of the baseline level).Beagles of the GA group were not treated with controlled hypotension(the initial concentration of SNP = 1μg/kg·min-1

and with an increase rate of 1μg/kg·min-1 until the controlled hypotension phase).In the TEAS group

TEAS [2Hz/100Hz

(4±1)mA]was applied to "Quchi"(LI11)

"Zusanli"(ST 36)

"Hegu"(LI4)

and "Sanyinjiao"(SP 6) from the beginning of the stable physiological condition phase to the end of the controlled-low mean arterial pressure(MAP) phase.Electrogastrogram(EGG) was recorded

and serum gastrin(GAS) and motilin(MTL) contents were detected by enzyme-linked immunosorbent assay.Results Compared with the basic level

the amplitude value of EGG at 60min after controlled hypotension in the GA+CHT group was significantly lower(P<0.05);while the amplitude level of EGG of the TEAS group at the time-point of MAP recovery was significantly higher than those of the GA and GA+CHT groups(P<0.05).No significant changes were found among the 3 groups in the frequency levels of EGG.At the time-points of MAP recovery and 2h after MAP recovery

serum MTL and GAS levels in the TEAS group were close to their basic level(P>0.05)

and serum GAS level at the time-point of 2h after MAP recovery in the TEAS group was considerably higher than those in the GA and GA+CHT groups(P<0.05).Twenty-four

48 and 72h after MAP recovery

serum GAS levels in the TEAS group were moderately(24h and 48h) and significantly(72h) higher than that of the basic level.No significant differences were found between the GA and GA+CHT groups in serum MTL and GAS levels at the time-points of 2

24

48 and 72h after MAP recovery(P>0.05).Conclusion TEAS combining with general anesthesia for controlled hypotension can improve the amplitude of EGG

and serum MTL and GAS contents

favoring the recovery of gastric dynamics and the functional protection of stomach.