Objective To observe the effect of electroacupuncture(EA) preconditioning of "Neiguan"(PC 6) on myocardial enzyme levels in acute recurrent myocardial ischemia rabbits so as to study its mechanisms underlying protection of ischemic myocardium.Methods A total of 40 healthy rabbits were randomized into normal control

myocardial ischemia(MI) model

EA preconditioning(EA-P)

recurrent MI(RMI)

EA-P+RMI groups.MI model was duplicated by auricular intravenous administration of Posterior Pituitary Injection(PPI

0.3 mL/kg).EA(2 Hz/70 Hz

2-4 mA)was applied to bilateral PC 6 for 30 min

once daily for 7 d before MI.The contents of serum aspartate aminotransferase(AST)

isoenzymes of creatine kinase(CK-MB)

lactate dehydrogenase(LDH)

creatine kinase(CK)

α-hydroxybutyrate dehydrogenase(α-HBD) were detected by automatic biochemistry analyzer.Results Following administration of PPI

ECG-STⅡ

serum AST

CK-MB

LDH

CK and α-HBD levels were increased significantly in the MI and RMI groups(P<0.01).Compared with the MI model group

ECG-STⅡ

serum AST

CK-MB

LDH

CK and α-HBD levels were down-regulated considerably in the EA-P group(P<0.01)

and ECG-STⅡ

serum AST

CK-MB

LDH

CK and α-HBD levels were obviously lower in the EA-P+RMI group than in the RMI group(P<0.01).No significant differences were found between the MI and RMI groups

and between EA-P and EA-P+RMI groups in the above-mentioned 6 indexes(P>0.05).Conclusion Electroacupuncture preconditioning has a remarkable effect in the prevention of ischemic myocardial injury of both acute and recurrent myocardial ischemia in the rabbit

which is closely associated with its functions in lowering serum AST

CK-MB

LDH

CK and α-HBD contents.