Objective To observe the effect of traditional manipulations of "Shaoshanhuo"(heat-producing needling) and "Toutianliang"(cool-producing needling) on body temperature and serum endotoxin level in heat syndrome rabbits.Methods Twenty-four Japanese rabbits were randomly divided into control

model

Shaoshanhuo and Toutianliang groups.Heat-syndrome model was established by subcutaneous injection of bacterium coli endotoxin solution(40 μg/mL

2 mL/kg).Heat-producing and cool-producing needling was applied to bilateral "Quchi"(LI 11) for 5 min

respectively.Rectal temperature was detected by using a thermometer

and serum endotoxin content assayed by using Limulus Ameboyte Lysate kit(luminescence measuring).Results In comparison with the control group

both rectal temperature and serum endotoxin levels were increased significantly in the model group(P<0.01).While compared to the model group

the rectal temperature and serum endotoxin levels were down-regulated considerably in both Shaoshanhuo and Toutianliang groups(P<0.05

P<0.01).The effect of the Toutianliang group was obviously superior to that of the Shaoshanhuo group in reducing serum endotoxin content(P<0.01).Conclusion Both heat-producing needling and cool-producing needling can lower rectal temperature and serum endotoxin levels in heat-syndrome rabbits

and the effect of cool-producing needling is relatively better in reducing endotoxin content.