Objective To observe changes of anesthesia analepsia time and hypothalamic and hippocampal corticotropin-releasing factor(CRF)protein expression after electroacupuncture(EA)intervention in acute traumatic rats

in order to explore its mechanism underlying relief of post-operative stress.Methods Twenty male Sprague-Dawley rats were randomly divided into normal control group

model group

sham EA group and EA group(5rats/group).Acute traumatic model was established by left lobectomy.For rats of the sham EA group

two filiform needles were inserted into the right"Zusanli"(ST 36)and"Sanyinjiao"(SP 6)respectively

without needle-twirling and without electrical stimulation.For rats of the EA group

the right ST36and SP 6were electrically stimulated at 2Hz/100Hz and 2mA for 30minutes after needle insertion.Anesthesia analepsia time was recorded and righting reflex was tested for the acute traumatized rats.The expression of CRF protein in the hypothalamus and hippocampus was analyzed with Western blot.Results The anesthesia analepsia time and righting reflex recovery time were significantly shorter in the EA group than in the model group and sham EA group(P<0.01).There was a close correlation between the anesthesia analepsia time and righting reflex recovery time(P<0.05).In comparison with the normal group

the expression level of hippocampal CRF protein was significantly decreased whereas that of hypothalamic CRF protein evidently increased in the model group(P<0.05).Compared with the model group

hippocampal CRF protein expression was considerably upregulated and hypothalamic CRF protein remarkably down-regulated in the EA group(P<0.05).A markedly negative correlation was found between hypothalamic CRF and hippocampal CRF protein expression

and between hippocampal CRF expression and the stimulating mode(P<0.001)

and a positive correlation was found between hypothalamic CRF expression and righting reflex

and between hippocampal CRF expression and EA intervention(P<0.01).Conclusion EA intervention can shorten anesthesia analepsia time and righting reflex recovery time in liver-traumatized rats

which is closely associated with its effects in upregulating hippocampal CRF protein expression and down-regulating hypothalamic CRF expression.It suggests that CRF mediated hippocampus-hypothalamus-pituitary-axis is involved in EA induced reduction of post-surgical stress.