通督调神针灸预处理对脑缺血再灌注大鼠相关微小RNA调控机制的研究

郑仕平; 韩为; 储浩然; 王颖; 张玲; 朱玲玲; 张国庆; 崔倩倩

doi:10.13702/j.1000-0607.2015.02.003

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通督调神针灸预处理对脑缺血再灌注大鼠相关微小RNA调控机制的研究

机制探讨 | 更新时间：2023-08-11

- 通督调神针灸预处理对脑缺血再灌注大鼠相关微小RNA调控机制的研究
- Effect of Electroacupuncture and Moxibustion Pretreatment on Expression of Cerebral microRNAs and Aquaporin Protein-4 in Cerebral Infarction Rats
- 针刺研究 2015年40卷第2期页码：99-103
- 作者机构：
  
  安徽中医药大学第二附属医院
- 作者简介：
- 基金信息：
  
  安徽省自然科学基金项目(No.1308085MH 174)
- DOI：10.13702/j.1000-0607.2015.02.003
  中图分类号：
- 纸质出版日期：2015
- 稿件说明：
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郑仕平, 韩为, 储浩然, 等. 通督调神针灸预处理对脑缺血再灌注大鼠相关微小RNA调控机制的研究[J]. 针刺研究, 2015,40(2):99-103.

ZHENG Shi-ping, HAN Wei, CHU Hao-ran, et al. Effect of Electroacupuncture and Moxibustion Pretreatment on Expression of Cerebral microRNAs and Aquaporin Protein-4 in Cerebral Infarction Rats[J]. Acupuncture research, 2015, 40(2): 99-103.
郑仕平, 韩为, 储浩然, 等. 通督调神针灸预处理对脑缺血再灌注大鼠相关微小RNA调控机制的研究[J]. 针刺研究, 2015,40(2):99-103. DOI： 10.13702/j.1000-0607.2015.02.003.

ZHENG Shi-ping, HAN Wei, CHU Hao-ran, et al. Effect of Electroacupuncture and Moxibustion Pretreatment on Expression of Cerebral microRNAs and Aquaporin Protein-4 in Cerebral Infarction Rats[J]. Acupuncture research, 2015, 40(2): 99-103. DOI： 10.13702/j.1000-0607.2015.02.003.

摘要

目的:观察通督调神针灸预处理对脑缺血再灌注大鼠相关微小RNA(microRNA

miRNAs)、水通道蛋白-4(Aquaporin-4

AQP 4)表达的影响

探讨通督调神针灸预处理脑保护的作用机制。方法 :Wistar大鼠随机选取10只作为空白组

其余120只分为电针预处理组、艾灸预处理组、阿司匹林预处理组及模型组各30只。采用Longa线栓法制备脑缺血再灌注模型。电针和艾灸预处理组取"百会""风府""大椎"穴。电针预处理组进行电针治疗

艾灸预处理组用清艾条悬灸治疗

阿司匹林预处理组用阿司匹林10mg/kg灌胃治疗

治疗7d后各组造模

并于再灌注24h后

运用实时荧光PCR和Western blot法分别检测皮质区相关miRNAs、AQP 4的表达。结果:与空白组相比

模型组miRNA 290、miRNA 494相对表达量均明显降低(P<0.01);与模型组相比

各预处理组miRNA 290、miRNA 494相对表达量均显著提高(P<0.01);与阿司匹林预处理组相比

电针预处理组和艾灸预处理组miRNA 290、miRNA 494相对表达量均显著提高(P<0.01);与艾灸预处理组相比

电针预处理组相对表达量显著提高(P<0.05)。与空白组相比

模型组AQP 4相对表达量显著提高(P<0.01);与模型组相比

各预处理组AQP 4相对表达量均显著降低(P<0.05

P<0.01);与阿司匹林预处理组相比

电针预处理组和艾灸预处理组AQP 4相对表达量均显著降低(P<0.01

P<0.05);与艾灸预处理组相比

电针预处理组相对表达量降低更明显(P<0.05)。结论:通督调神针灸预处理是预防脑梗死的有效方法

通督调神针灸预处理脑保护机制之一可能是通过提高miRNA 290、miRNA 494的表达

降低AQP 4相对表达量

诱导脑缺血耐受

减轻脑水肿实现的。

Abstract

Objective The present study aimed at observing the effect of moxibustion pretreatment on the expression of cerebral microRNAs and Aquaporin protein-4(AQP 4)in rats with cerebral ischemia and reperfusion(CI/R)

so as to reveal its mechanism underlying improvement of cerebral infarction.Methods A total of 130 Wistar rats were randomly divided into blank control(n=10)

CI/R model(n=30)

electroacupuncture(EA

n=30)

moxibustion(n=30)

Aspirin groups(n=30).Before modeling

EA(2Hz/5Hz

1-2mA)or moxibustion was applied to"Baihui"(GV 20)

"Fengfu"(GV 16)and"Dazhui"(GV 14)for 20 min

once daily for 7days.The rats of the Asprin group were treated by intragastric administration of Aspirin(10mg/kg

1mg/mL)

once daily for 7days before modeling.The CI/R model was established by occlusion of the bilateral carotid arteries.The expression levels of cerebral miRNAs and AQP 4were detected by real-time PCR and Western blot

respectively.ResultsCompared with the blank control group

the expression levels of cerebral miRNA 290 and miRNA 494 were significantly reduced

while that of AQP 4was obviously up-regulated in the model group(P<0.01).After pretreatment with EA and moxibustion

the relative expression levels of miRNA 290 and miRNA 494 were significantly higher in the EA

moxibustion and Aspirin pretreatment groups than in the model group(P<0.01)

while cortical AQP 4expression levels were significantly lower in the EA

moxibustion and Aspirin pretreatment groups than in the model group(P<0.01

P<0.05).The effects of both EA and moxibustion groups were significantly superior to those of Aspirin pretreatment group in up-regulating expression of miRNA 290 and miRNA 494 and down-regulating expression of AQP 4(P<0.01

P<0.05).In addition

the EA pretreatment was markedly superior to moxibustion pretreatment in the aforementioned effects(P<0.05).Conclusion EA pretreatment of GV 14

GV 16 and GV 20 can effectively up-regulate cerebral cortical miRNA 290 and miRNA 494 and down-regulate AQP 4in CI/R rats

which may contribute to its effect in preventing the cerebral tissue from ischemia/reperfusion injury.

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