电针及天麻多糖对局灶性脑缺血大鼠缺血灶周围额叶皮质巢蛋白、干细胞因子表达的影响

赵健; 黄锐; 李怀斌; 熊克仁

doi:10.13702/j.1000-0607.2015.02.005

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电针及天麻多糖对局灶性脑缺血大鼠缺血灶周围额叶皮质巢蛋白、干细胞因子表达的影响

机制探讨 | 更新时间：2023-08-11

- 电针及天麻多糖对局灶性脑缺血大鼠缺血灶周围额叶皮质巢蛋白、干细胞因子表达的影响
- Effect of Electroacupuncture Intervention Combined with Polysaccharide of Gastrodia Elata Blume on Expression of Nestin and Stem Cell Factor around the Ischemic Locus of Frontal Lobe Cortex in Local Cerebral Ischemia Rats
- 针刺研究 2015年40卷第2期页码：108-112
- 作者机构：
  
  皖南医学院解剖学教研室
- 作者简介：
- 基金信息：
  
  安徽省高校省级自然科学项目(No.KJ 2012Z423);皖南医学院中青年科研基金(No.WK 201003)
- DOI：10.13702/j.1000-0607.2015.02.005
  中图分类号：
- 纸质出版日期：2015
- 稿件说明：
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赵健, 黄锐, 李怀斌, 等. 电针及天麻多糖对局灶性脑缺血大鼠缺血灶周围额叶皮质巢蛋白、干细胞因子表达的影响[J]. 针刺研究, 2015,40(2):108-112.

ZHAO Jian, HUANG Rui, LI Huai-bin, et al. Effect of Electroacupuncture Intervention Combined with Polysaccharide of Gastrodia Elata Blume on Expression of Nestin and Stem Cell Factor around the Ischemic Locus of Frontal Lobe Cortex in Local Cerebral Ischemia Rats[J]. Acupuncture research, 2015, 40(2): 108-112.
赵健, 黄锐, 李怀斌, 等. 电针及天麻多糖对局灶性脑缺血大鼠缺血灶周围额叶皮质巢蛋白、干细胞因子表达的影响[J]. 针刺研究, 2015,40(2):108-112. DOI： 10.13702/j.1000-0607.2015.02.005.

ZHAO Jian, HUANG Rui, LI Huai-bin, et al. Effect of Electroacupuncture Intervention Combined with Polysaccharide of Gastrodia Elata Blume on Expression of Nestin and Stem Cell Factor around the Ischemic Locus of Frontal Lobe Cortex in Local Cerebral Ischemia Rats[J]. Acupuncture research, 2015, 40(2): 108-112. DOI： 10.13702/j.1000-0607.2015.02.005.

摘要

目的:观察电针、天麻多糖及电针+天麻多糖对局灶性脑缺血大鼠缺血灶周围额叶皮质巢蛋白(Nestin)和干细胞因子(stem cell factor

SCF)表达的影响

探讨针药结合治疗局灶性脑缺血的机制。方法:成年SD大鼠随机分为正常组、模型组、电针组、天麻多糖组和电针+天麻多糖组

每组8只。以线栓法建立大脑中动脉栓塞模型。模型建立后

电针组和电针+天麻多糖组大鼠给予"百会""足三里"穴电针刺激

时间30min

每天1次

连续14d;天麻多糖组和电针+天麻多糖组大鼠给予天麻多糖100mg/kg灌胃

每天1次

连续14d。采用免疫组织化学SABC法与图像分析系统观察大鼠脑缺血灶周围额叶皮质Nestin和SCF的表达。结果:与正常组比较

模型组缺血灶周围额叶皮质Nestin和SCF阳性表达增加(P<0.05);与模型组比较

电针组、天麻多糖组和电针+天麻多糖组缺血灶周围额叶皮质Nestin和SCF阳性表达显著增加(P<0.05);电针+天麻多糖组阳性表达显著多于电针组或天麻多糖组(P<0.05)。结论:电针与天麻多糖联合应用可明显上调缺血灶周围额叶皮质Nestin和SCF的表达

其作用优于单用电针或天麻多糖

提示电针可能促进天麻多糖在体内的吸收

对促进缺血灶周围额叶皮质内源性神经干细胞的增殖有协同增效作用。

Abstract

Objective To observe the effect of electroacupuncture(EA)

Polysaccharide of Gastrodia elata Blume(PGB)

and EA+PGB on the expression of Nestin and stem cell factor(SCF)in the frontal lobe cortex around the ischemic loci of cerebral ischemia(CI)rats

so as to explore its mechanisms underlying improvement of CI.Methods A total of 40 SpragueDawley adult rats were randomly divided into normal control

CI model

EA intervention

PGB intervention and EA+PGB groups(n=8in each group).The CI model was prepared by middle cerebral artery occlusion.EA(2Hz

2V)was applied to"Baihui"(GV 20)and left"Zusanli"(ST 36)for 30 min

once daily for 14 days.Rats of the PGB and EA+PGB groups were treated by gastrogavage of PGB at a dose of 100mg/kg

once daily for 14 successive days.The expression of Nestin and SCF in the frontal lobe around the ischemic loci of the frontal lobe was detected by immunohistochemistry.Results Compared with the normal control group

the expression levels of regional cerebral cortical Nestin and SCF proteins were significantly increased in the model group(P<0.05).After the treatment

the expression levels of Nestin and SCF were significantly further up-regulated in the EA

PGB and EA+PGB groups in comparison with the model group(P<0.05).Conclusion EA combined with PGB can significantly up-regulate the expression of Nestin and SCF in the frontal lobe around the ischemic loci in cerebral ischemia rats

which may contribute to their function in improving CI.

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