Objective To observe the effect of electroacupuncture(EA)stimulation at Zhongwan(CV 12)on the skin microcirculatory blood flow along the Conception Vessel(CV)in yin-deficiency constitution volunteers.Methods A total of 16yin-deficiency constitution volunteers(9woman and 6men)from 200 students of Fujian University of Traditional Chinese Medicine finished a body constitution questionnaire and showed a yin-deficiency constitution.Skin microcirculatory blood perfusion units(MBPU)at Danzhong(CV 17)

Xiawan(CV 10)

and Qihai(CV 6)of the CV were measured by using a Laser Doppler Flowmetry(Peri Flux 5000)in normal constitution

before and after EA stimulation of Zhongwan(CV 12)for 20 min.Results Under normal condition

the cutaneous MBPU values were significantly lower in the Qihai(CV 6)than in both Danzhong(CV 17)and Xiawan(CV 10)(P<0.01).After EA stimulation of CV 12 for 20min

the MBPU values were significantly decreased in the CV 17 and CV 6acupoint regions compared with pre-stimulation(P<0.05).No significant changes of skin MBPU were found in Xiawan(CV 10)after EA stimulation of CV 12(P>0.05).Conclusion EA at Zhongwan(CV 12)can reduce the skin MBPU values of CV 6and CV 17 of the Conception Vessel in yin-deficiency constitution volunteers.