电针对足底切口痛大鼠下丘脑及脊髓β-内啡肽的影响

贾旺华; 迟文英; 娄超; 王桂芝

doi:10.13702/j.1000-0607.2016.03.007

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电针对足底切口痛大鼠下丘脑及脊髓β-内啡肽的影响

机制探讨 | 更新时间：2023-08-11

- 电针对足底切口痛大鼠下丘脑及脊髓β-内啡肽的影响
- Effect of Electroacupuncture Intervention on β-endorphin Levels of Hypothalamus and Spinal Cord Tissues in Rats with Pelmatic Incisional Pain
- 针刺研究 2016年41卷第3期页码：225-229
- 作者机构：
  
  潍坊医学院麻醉学系临床麻醉实验室
- 作者简介：
- 基金信息：
- DOI：10.13702/j.1000-0607.2016.03.007
  中图分类号：
- 纸质出版日期：2016
- 稿件说明：
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贾旺华, 迟文英, 娄超, 等. 电针对足底切口痛大鼠下丘脑及脊髓β-内啡肽的影响[J]. 针刺研究, 2016,41(3):225-229.

JIA Wang-hua, CHI Wen-ying, LOU Chao, et al. Effect of Electroacupuncture Intervention on β-endorphin Levels of Hypothalamus and Spinal Cord Tissues in Rats with Pelmatic Incisional Pain[J]. Acupuncture research, 2016, 41(3): 225-229.
贾旺华, 迟文英, 娄超, 等. 电针对足底切口痛大鼠下丘脑及脊髓β-内啡肽的影响[J]. 针刺研究, 2016,41(3):225-229. DOI： 10.13702/j.1000-0607.2016.03.007.

JIA Wang-hua, CHI Wen-ying, LOU Chao, et al. Effect of Electroacupuncture Intervention on β-endorphin Levels of Hypothalamus and Spinal Cord Tissues in Rats with Pelmatic Incisional Pain[J]. Acupuncture research, 2016, 41(3): 225-229. DOI： 10.13702/j.1000-0607.2016.03.007.

摘要

目的:观察电针对足底切口痛大鼠痛阈、下丘脑和脊髓内β-内啡肽(β-EP)、中缝背核(DRN)内5-羟色胺(5-HT)的影响

探讨电针对足底切口痛大鼠的镇痛作用机制。方法:Wistar大鼠随机分为正常组、模型组、电针穴位组、电针非穴位组

每组8只。采用足底切口复制痛模型。电针穴位组选取右侧"足三里""昆仑"穴

电针非穴位组选择两穴位旁开3mm非穴位处

每天电针1次

每次20min

治疗3次。观察各组大鼠痛阈变化

采用酶联免疫分析法、免疫组化法测定下丘脑、脊髓内β-EP以及DRN内5-HT的表达水平。结果:模型组大鼠机械痛阈及热痛阈均较正常组明显降低(P<0.05);下丘脑β-EP含量较正常组明显升高

β-EP阳性细胞数明显增多(P<0.05);DRN内5-HT表达水平较正常组明显升高(P<0.05)。治疗后

电针穴位组大鼠的机械痛阈和热痛阈明显高于模型组和电针非穴位组(P<0.05);下丘脑β-EP含量和阳性细胞数较模型组与电针非穴位组明显升高(P<0.05);DRN内5-HT表达水平较模型组和电针非穴位组明显升高(P<0.05)。各组脊髓内β-EP含量的变化差异无统计学意义(P>0.05)。结论:电针对大鼠足底切口疼痛的镇痛作用可能是通过提高下丘脑β-EP含量进而激活DRN内5-HT的表达介导。

Abstract

Objective To observe the effect of electroacupuncture(EA)intervention on pain thresholds(PT)and contents ofβ-endorphin(EP)in the hypothalamus and spinal cord

and the expression of 5-HT in the dorsal raphe nucleus(DRN)in rats with pelmatic incisional pain

so as to investigate the underlying mechanisms of acupuncture in reducing post-operative pain.Methods Wistar rats were randomized into normal control

model

EA and non-acupoint groups(n=8/group).The pelmatic pain model was induced by making an incision(about 1cm in length

to the fascia and muscle layers)from the heel towards the toes.EA(2Hz

1.5-2V)was applied to "Zusanli"(ST 36)and "Kunlun"(BL 60)or non-acupoint(about 3mm beside the ST 36 and BL 60)on the affected side for 20 min

once daily for three days.The thermal PT and mechanical PT were measured before and after operation and after EA.The contents ofβ-EP in hypothalamus and L3-S4 spinal cord were detected using enzyme linked immunosorbent assay(ELISA)and the expressions ofβ-EP in hypothalamus and 5-HT in DRN were measured with immunohistochemistry.Results After EA intervention

the markedly decreased mechanical and thermal pain thresholds on day 1and 3after paw incision were significantly increased in the EA group(P<0.05)

but not in the non-acupoint group(P>0.05).The hypothalamicβ-EP content was significantly higher in the model group than in the normal group(P<0.05)

and further up-regulated in the EA group(not the non-acupoint group)than in the model group(P<0.05).In addition

the hypothalamicβ-EP immunoreactive(IR)-positive cell number and 5-HT immunoactivity level in DRN were also considerably up-regulated in the EA group(P<0.05)but not in the non-acupoint group(P>0.05).No significant changes were found in the lumbar spinalβ-EP contents in the model

EA and non-acupoint groups(P>0.05).Conclusion EA stimulation of"Zusanli"(ST 36)and "Kunlun"(BL 60)has an analgesic effect in pelmatic incision pain rats

which may be related to its effects in raising the level of hypothalamicβ-EP and the expression of 5-HT in DRN.

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