直接灸“肝俞”对原发性肝癌癌前病变大鼠T淋巴细胞亚群的影响

闫亚南; 王宁; 王卓昱; 田瑶; 成宇镇; 马惠芳; 侯中伟; 马佳佳; 关翰宇

doi:10.13702/j.1000-0607.2016.04.007

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直接灸“肝俞”对原发性肝癌癌前病变大鼠T淋巴细胞亚群的影响

机制探讨 | 更新时间：2023-08-11

- 直接灸“肝俞”对原发性肝癌癌前病变大鼠T淋巴细胞亚群的影响
- Effects of Direct Moxibustion of “Ganshu”(BL 18)on the Contents of T cells in Peripheral Blood in Rats with Precancerous Lesion of Primary Hepatocellular Carcinoma
- 针刺研究 2016年41卷第4期页码：321-326
- 作者机构：
  
  北京中医药大学针灸推拿学院
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年科学基金项目(No.81202760)
- DOI：10.13702/j.1000-0607.2016.04.007
  中图分类号：
- 纸质出版日期：2016
- 稿件说明：
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闫亚南, 王宁, 王卓昱, 等. 直接灸“肝俞”对原发性肝癌癌前病变大鼠T淋巴细胞亚群的影响[J]. 针刺研究, 2016,41(4):321-326.

YAN Ya-nan, WANG Ning, WANG Zhuo-yu, et al. Effects of Direct Moxibustion of “Ganshu”(BL 18)on the Contents of T cells in Peripheral Blood in Rats with Precancerous Lesion of Primary Hepatocellular Carcinoma[J]. Acupuncture research, 2016, 41(4): 321-326.
闫亚南, 王宁, 王卓昱, 等. 直接灸“肝俞”对原发性肝癌癌前病变大鼠T淋巴细胞亚群的影响[J]. 针刺研究, 2016,41(4):321-326. DOI： 10.13702/j.1000-0607.2016.04.007.

YAN Ya-nan, WANG Ning, WANG Zhuo-yu, et al. Effects of Direct Moxibustion of “Ganshu”(BL 18)on the Contents of T cells in Peripheral Blood in Rats with Precancerous Lesion of Primary Hepatocellular Carcinoma[J]. Acupuncture research, 2016, 41(4): 321-326. DOI： 10.13702/j.1000-0607.2016.04.007.

摘要

目的:观察不同灸量对原发性肝癌癌前病变模型大鼠外周血中T淋巴细胞亚群含量的影响

探讨直接灸对肝癌大鼠免疫功能的影响。方法:雄性Wistar大鼠随机分为空白组(10只)、模型组(15只)、直接灸1组(15只)、直接灸2组(15只)以及隔姜灸组(15只)

采用腹腔注射二乙基亚硝胺法逐步诱导肝癌模型

为期10周。直接灸1组、直接灸2组造模的同时分别给予直接灸"肝俞"穴3壮/穴、6壮/穴干预

隔日1次;隔姜灸组造模开始的同时运用"肝俞"隔姜灸法进行干预

3壮/穴

隔日1次

连续10周。运用流式细胞仪检测大鼠外周血中T淋巴细胞亚群CD 3

、CD 4+、CD 4

、CD 8+、CD 8

的含量

HE染色观察大鼠肝脏组织形态学变化。结果:与空白组比较

模型组CD 3+的含量

HE染色观察大鼠肝脏组织形态学变化。结果:与空白组比较

模型组CD 3

、CD 4+、CD 4

T细胞含量降低(P

0.05

0.01);直接灸干预后其含量显著升高(P

0.05

0.01);而隔姜灸组与模型组比

CD 3+T细胞含量差异无统计学意义(P

0.05)

CD 4+T细胞含量降低(P

0.05

0.01);直接灸干预后其含量显著升高(P

0.05

0.01);而隔姜灸组与模型组比

CD 3+T细胞含量差异无统计学意义(P

0.05)

CD 4

T细胞含量显著升高(P

0.01)。与空白组比较

模型组CD 8+T细胞含量显著升高(P

0.01)。与空白组比较

模型组CD 8

T细胞含量升高(P

0.01);直接灸1、2组及隔姜灸组CD 8+T细胞含量与模型组比显著降低(P

0.01)。与模型组比较

艾灸后CD 4+T细胞含量升高(P

0.01);直接灸1、2组及隔姜灸组CD 8+T细胞含量与模型组比显著降低(P

0.01)。与模型组比较

艾灸后CD 4

/CD 8+/CD 8

比值显著升高(P

0.01)。在病理改变方面

艾灸各干预组镜下癌细胞分化程度高

异型性低。结论:直接灸和隔姜灸对肝癌癌前病变病理形态学变化有改善作用

可能与调节外周血中CD 3+比值显著升高(P

0.01)。在病理改变方面

艾灸各干预组镜下癌细胞分化程度高

异型性低。结论:直接灸和隔姜灸对肝癌癌前病变病理形态学变化有改善作用

可能与调节外周血中CD 3

、CD 4+、CD 4

、CD 8+、CD 8

T细胞的含量

提高充分免疫应答效应

增强免疫监视

避免肿瘤细胞逃逸相关。Objective To observe the effect of moxibustion stimulation of"Ganshu"(BL 18)region on contents of T cells in the peripheral blood in rats with Diethylnitrosamine(DEN)-induced hepatocellular carcinoma(HCC)

so as to explore its effective in improving immunoregulatory function.Methods Seventy male Wistar rats were randomly divided into control group(n=10)

model group(n=15)

direct moxibustion-15sgroup(n=15)

direct moxibustion-30sgroup(n=15)and ginger-separated moxibustion group(n=15).The primary HCC precancerous lesion model was established by intraperitoneal injection of DEN(50mg/kg)

once every 3days for 10 weeks.Moxibustion was applied to bilateral"Ganshu"(BL 18)for about 15min(3 moxacones)

or 30min(six moxa-cones)

with or without ginger-slice separation

once every other day for 10 consecutive weeks.The contents of T cells of CD 3+T细胞的含量

提高充分免疫应答效应

增强免疫监视

避免肿瘤细胞逃逸相关。

Abstract

Objective To observe the effect of moxibustion stimulation of"Ganshu"(BL 18)region on contents of T cells in the peripheral blood in rats with Diethylnitrosamine(DEN)-induced hepatocellular carcinoma(HCC)

so as to explore its effective in improving immunoregulatory function.Methods Seventy male Wistar rats were randomly divided into control group(n=10)

model group(n=15)

direct moxibustion-15sgroup(n=15)

direct moxibustion-30sgroup(n=15)and ginger-separated moxibustion group(n=15).The primary HCC precancerous lesion model was established by intraperitoneal injection of DEN(50mg/kg)

once every 3days for 10 weeks.Moxibustion was applied to bilateral"Ganshu"(BL 18)for about 15min(3 moxacones)

or 30min(six moxa-cones)

with or without ginger-slice separation

once every other day for 10 consecutive weeks.The contents of T cells of CD 3

CD 4+

CD 4

CD 8+and CD 4+

CD 8+and CD 4

/CD 8+/CD 8

ratio in the peripheral blood were detected with Flow Cytometey(FCM)

pathological changes of liver were observed by light microscope after hematoxylin-eosin(HE)stain.ResultsCompared to the control group

the contents of blood CD 3+ ratio in the peripheral blood were detected with Flow Cytometey(FCM)

pathological changes of liver were observed by light microscope after hematoxylin-eosin(HE)stain.ResultsCompared to the control group

the contents of blood CD 3

and CD 4+ and CD 4

T cells and ratio of CD 4+ T cells and ratio of CD 4

/CD 8+/CD 8

were significantly down-regulated

while that of CD 8+ were significantly down-regulated

while that of CD 8

T cells was obviously increased in the model group(P

0.05

0.01).After moxibustion intervention

the decreased CD 3+T cells was obviously increased in the model group(P

0.05

0.01).After moxibustion intervention

the decreased CD 3

and CD 4+ and CD 4

T cells and CD 4+/CD 8+ T cells and CD 4+/CD 8

levels and the increased CD 8+ levels and the increased CD 8

T cell contents were reversed in all the 3 moxibustion groups(P

0.05

0.01)

except CD 3+ T cell contents were reversed in all the 3 moxibustion groups(P

0.05

0.01)

except CD 3

in the ginger-separated moxibustion group(P

0.05).There were no significant differences among the three moxibustion groups in the CD 3+ in the ginger-separated moxibustion group(P

0.05).There were no significant differences among the three moxibustion groups in the CD 3

CD 4+

CD 4

CD 8+

CD 8

and CD 4+ and CD 4

/CD 8+/CD 8

levels(P

0.05).In addition

the pathological changes of liver tissue as central vein deviation or absence

disordered arrangement of hepatic cords

narrowing of the hepatic sinusoid

hyperplasia of collagen fibers

formation of tuberosis

unevenness of liver cells with nuclear anachromasis and higher heteromorphism

and macronucleus oncocytes in primary HCC rats were not observed or milder after moxibustion intervention.Conclusion Direct moxibustion and ginger-separated moxibustion can improve pathological changes of hepatic cells in rats with HCC

which may be associated with its actions in raising blood CD 3+ levels(P

0.05).In addition

the pathological changes of liver tissue as central vein deviation or absence

disordered arrangement of hepatic cords

narrowing of the hepatic sinusoid

hyperplasia of collagen fibers

formation of tuberosis

unevenness of liver cells with nuclear anachromasis and higher heteromorphism

which may be associated with its actions in raising blood CD 3

and CD 4+ and CD 4

T cell contents and reducing CD 8+T cell contents and reducing CD 8

T levels to improve immune function.

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