Objective To observe the different effects of electroacupucnture of"Neiguan"(PC 6)grouping with other acupoints on myocardial ischemia(MI)injury

so as to provide evidence for clinical acupoint formulas.Methods Forty Wistar male rats were randomly divided into normal

model

EA 1[bilateral"Neiguan"(PC 6)

bilateral"Zusanli"(ST 36)

"Guanyuan"(CV 4)]

and EA 2 [bilateral"Neiguan"(PC 6)

bilateral"Shenmen"(HT 7)

"Danzhong"(CV 17)]groups

10 rats in each group.The MI model was established by subcutaneous injection with ISO solution(5mL/kg)

once per day for 7days.Both EA 1and EA 2groups were subjected to EA(2Hz/100 Hz

1mA)for 10 min before subcutaneous injection

once daily for 21 days.Electrocardiogram(ECG)was recorded after treatment.Serum cTnT was detected by biochemistry.SOD

GSH-Px and MDA in myocardial tissues of left ventricular were measured by ELISA

and the ultrastructural changes of myocardial cells in the left ventricular were observed by electron microscope.Results Compared with the normal group

the amplitude of S-T segment and HR of ECG

cTnT and MDA contents were significantly increased in the model group(P<0.01)accompanied with seriously destroyed ultrastructure in the myocardial tissue

while SOD and GSH-Px levels were lower than those in the normal group(P<0.01).However

EA treatments reversed all the abnormal changes in the model group(P<0.05).There was no significant difference between EA 1and EA 2groups in the amplitude of S-T segment and HR(P>0.05);cTnT and MDA levels in the EA 1group were lower than those in the EA 2group(P <0.05)

while SOD and GSH-Px levels were higher than those in the EA 2group(P<0.05)

accompanied with lighter degree of ultrastructure damage in the myocardial tissue in the EA 1group.ConclusionEA can prevent myocardial ischemic injury

and the effect of EA 1treatment was better than that of EA 2.