Objective To observe the effect of low-frequency or high-frequency electroacupuncture(EA)stimulation of"Neiguan"(PC 6)on changes of skin blood perfusion volume of heart-related and heart-irrelevant acupoints in rats with normal or ischemic myocardium.Methods Wistar rats were randomly divided into the control

sham-operation

model

low-frequency EA and high-frequency EA groups(n=8 in each group).The myocardial ischemic(MI)model was established by occlusion of the anterior descending branch of the left coronary artery.2 Hz and 100 Hz EA was respectively applied to left PC 6 for 20 min

once daily

a total of three times.Electrocardiogram(ECG)was recorded after the last treatment.Serum cardiac troponin T(cTnT)contents were assayed by ELISA.The skin blood perfusion volumes of bilateral PC 6

"Zusanli"(ST 36)and "Yanglingquan"(GB 34)were observed by laser speckle contrast imaging.Results In comparison with the control and sham-operation groups

the differences of"J point"and contents of serum cTnT were significantly increased in the model group(P<0.01).After EA

the differences of"J point"and contents of serum cTnT were significantly decreased in both low-frequency and high-frequency EA groups(P<0.01

P<0.05).Compared with the control group

the skin blood perfusion volumes of bilateral PC 6 and ST 36 decreased obviously in rats of the model group(P<0.01).After EA

the skin blood perfusion volumes were markedly up-regulated in the areas of PC 6 and ST 36(P<0.01).There were no significant changes in that of GB 34 among the 5 groups(P>0.05).Conclusion Skin blood perfusion volumes around PC 6 and ST 36 can specifically reflect the change of the myocardial state.