电针“中髎”“下髎”对慢传输型便秘大鼠结肠5-羟色胺信号系统及粪便短链脂肪酸的影响

马嘉泽; 陈鹏; 张加敏; 丁康; 周惠芬

doi:10.13702/j.1000-0607.20211058

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电针“中髎”“下髎”对慢传输型便秘大鼠结肠5-羟色胺信号系统及粪便短链脂肪酸的影响

机制探讨 | 更新时间：2023-08-10

- 电针“中髎”“下髎”对慢传输型便秘大鼠结肠5-羟色胺信号系统及粪便短链脂肪酸的影响
- Effects of electroacupuncture at “Zhongliao” and “Xialiao” on serotonin signaling system and short-chain fatty acids in slow transit constipation rats
- 针刺研究 2022年47卷第12期页码：1101-1106
- 作者机构：
  
  1. 南京中医药大学附属南京中医院
  2. 南京中医药大学附属南京中医院盆底中心
- 作者简介：
- 基金信息：
  
  南京市中医药青年人才项目(No.ZYQ20030);江苏省研究生实践创新计划项目(No.SJCX20＿0611)
- DOI：10.13702/j.1000-0607.20211058
  中图分类号：
- 纸质出版日期：2022
- 稿件说明：
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马嘉泽, 陈鹏, 张加敏, 等. 电针“中髎”“下髎”对慢传输型便秘大鼠结肠5-羟色胺信号系统及粪便短链脂肪酸的影响[J]. 针刺研究, 2022,47(12):1101-1106.

MA Jia-ze, CHEN Peng, ZHANG Jia-min, et al. Effects of electroacupuncture at “Zhongliao” and “Xialiao” on serotonin signaling system and short-chain fatty acids in slow transit constipation rats[J]. Acupuncture research, 2022, 47(12): 1101-1106.
马嘉泽, 陈鹏, 张加敏, 等. 电针“中髎”“下髎”对慢传输型便秘大鼠结肠5-羟色胺信号系统及粪便短链脂肪酸的影响[J]. 针刺研究, 2022,47(12):1101-1106. DOI： 10.13702/j.1000-0607.20211058.

MA Jia-ze, CHEN Peng, ZHANG Jia-min, et al. Effects of electroacupuncture at “Zhongliao” and “Xialiao” on serotonin signaling system and short-chain fatty acids in slow transit constipation rats[J]. Acupuncture research, 2022, 47(12): 1101-1106. DOI： 10.13702/j.1000-0607.20211058.

摘要

目的:观察电针“中髎”“下髎”对慢传输型便秘(STC)大鼠结肠5-羟色胺(5-HT)信号系统及粪便中短链脂肪酸(SCFA)的影响，探讨电针治疗STC的作用机制。方法:SD大鼠随机分为正常组、模型组、阳性药组及电针组，每组8只。采用洛哌丁胺灌胃建立STC大鼠模型。阳性药组予6 g/kg乳果糖灌胃治疗，电针组电针“中髎”“下髎”

每次30 min

两组均每日治疗1次，连续干预14 d。观察各组大鼠首粒黑便排出时间及粪便含水率；Western blot法检测各组大鼠结肠组织中5-羟色胺4受体(5-HT4R)、色氨酸羟化酶1(TPH1)、5-HT转运体(SERT)相对表达量；ELISA法检测各组大鼠血清P物质(SP)、血管活性肠肽(VIP)含量；高效液相色谱-质谱联用法检测各组大鼠结肠组织中5-HT含量及粪便中SCFA含量。结果:与正常组比较，模型组大鼠首粒黑便排出时间延长(P<0.05)

粪便含水率降低(P<0.05);结肠组织中5-HT含量及5-HT4R、TPH1、SERT蛋白表达量降低(P<0.05);血清SP含量降低(P<0.05)

VIP含量升高(P<0.05);粪便SCFA含量(除异丁酸外)均显著降低(P<0.05)。与模型组比较，电针组和阳性药组大鼠首粒黑便排出时间缩短(P<0.05)

粪便含水量升高(P<0.05);结肠组织中5-HT含量及5-HT4R、TPH1、SERT蛋白表达量升高(P<0.05);粪便中乙酸及丁酸含量均明显升高(P<0.05);电针组血清SP含量升高(P<0.05)

VIP含量降低(P<0.05)。电针组大鼠血清VIP含量较阳性药组显著降低(P<0.05);结肠组织TPH1、SERT蛋白表达量较阳性药组显著升高(P<0.05)。结论:电针“中髎”“下髎”可以通过干预5-HT信号系统的多个环节促进肠道动力，从而缓解STC。

Abstract

Objective To observe the effect of electroacupuncture(EA) at “Zhongliao”(BL33) and “Xialiao”(BL34) on the 5-hydroxytryptamine(5-HT) signaling system in colon tissue and short-chain fatty acids in feces of rats with slow transit constipation(STC)

so as to explore the underlying mechanisms of EA in the treatment of STC. Methods A total of 32 SD rats were randomly divided into normal

model

drug control and EA groups

with 8 rats in each group. The STC model was established by intragastric administration of loperamide for 14 days. The EA stimulation(2 Hz/15 Hz) was performed at bilateral BL33 and BL34 for 30 min

once a day for 14 days. The first black stool de-fecation time and fecal water content were detected after treatment. The expressions of 5-hydroxytryptamine 4 receptor(5-HT4 R)

tryptophan hydroxylase 1(TPH1) and 5-HT transporter(SERT) in colon tissues were detected by Western blot. The contents of substance P(SP) and vasoactive intestinal peptide(VIP) in serum were detected by ELISA. The contents of 5-HT in colon tissue and short chain fatty acid(SCFA) in feces were detected by mass spectrometry. Results Compared with the normal group

the fecal water content

the expressions of 5-HT

5-HT4 R

TPH1 and SERT in colon tissue

the content of serum SP were significantly decreased(P<0.05)

the first black stool de-fecation time

and the content of serum VIP was significantly increased(P<0.05)

the contents of SCFA in feces were significantly decreased except isobutyric acid(P<0.05) in the model group. Compared with the model group

the fecal water content

the expressions of 5-HT

5-HT4 R

TPH1 and SERT in colon tissues

the contents of acetic acid and butyrate in feces were significantly increased(P<0.05) in the EA and drug control groups

the first black stool defecation time was decreased(P<0.05) in the EA and drug control groups

and the content of serum SP was increased and the content of serum VIP was decreased(P<0.05) in the EA group. Compared with the drug control group

the content of serum VIP was significantly decreased(P<0.05)

and the expressions of TPH1 and SERT in colon tissue were significantly increased(P<0.05) in the EA group. Conclusion EA at BL33 and BL34 can promote intestinal motility by intervening multiple links of 5-HT signaling system in treating STC.

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