电针对偏头痛大鼠行为学及中脑导水管周围灰质小胶质细胞激活和P2X7受体表达的影响

裴培; 陈怀珍; 崔圣玮; 张帅帅; 杨文明; 王麟鹏

doi:10.13702/j.1000-0607.20211059

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电针对偏头痛大鼠行为学及中脑导水管周围灰质小胶质细胞激活和P2X7受体表达的影响

机制探讨 | 更新时间：2023-08-10

- 电针对偏头痛大鼠行为学及中脑导水管周围灰质小胶质细胞激活和P2X7受体表达的影响
- Effects of electroacupuncture on ethology, microglia activation and P2X7 receptor expression in periaqueductal gray in rats with migraine
- 针刺研究 2022年47卷第12期页码：1054-1059
- 作者机构：
  
  1. 安徽中医药大学第一附属医院脑病中心
  2. 安徽中医药大学研究生院
  3. 首都医科大学附属北京中医医院针灸科
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(No.81803903);安徽中医药大学临床科研项目(No.2021yfylc22)
- DOI：10.13702/j.1000-0607.20211059
  中图分类号：
- 纸质出版日期：2022
- 稿件说明：
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裴培, 陈怀珍, 崔圣玮, 等. 电针对偏头痛大鼠行为学及中脑导水管周围灰质小胶质细胞激活和P2X7受体表达的影响[J]. 针刺研究, 2022,47(12):1054-1059.

PEI Pei, CHEN Huai-zhen, CUI Sheng-wei, et al. Effects of electroacupuncture on ethology, microglia activation and P2X7 receptor expression in periaqueductal gray in rats with migraine[J]. Acupuncture research, 2022, 47(12): 1054-1059.
裴培, 陈怀珍, 崔圣玮, 等. 电针对偏头痛大鼠行为学及中脑导水管周围灰质小胶质细胞激活和P2X7受体表达的影响[J]. 针刺研究, 2022,47(12):1054-1059. DOI： 10.13702/j.1000-0607.20211059.

PEI Pei, CHEN Huai-zhen, CUI Sheng-wei, et al. Effects of electroacupuncture on ethology, microglia activation and P2X7 receptor expression in periaqueductal gray in rats with migraine[J]. Acupuncture research, 2022, 47(12): 1054-1059. DOI： 10.13702/j.1000-0607.20211059.

摘要

目的:观察电针“风池”对反复发作性偏头痛大鼠行为学、中脑导水管周围灰质(PAG)小胶质细胞激活及嘌呤能离子通道型受体7(P2X7R)表达的影响，探讨电针抗偏头痛中枢敏化的可能机制。方法:SD大鼠随机分为对照组、模型组、电针组，每组12只。反复硬脑膜电刺激诱导反复发作性偏头痛大鼠模型。电针组电针“风池”

每次10 min

每日1次，连续治疗9 d。电子von-Frey测痛仪检测各组大鼠第0、2、4、6、8天面部及后足底机械痛阈；免疫荧光染色法检测各组大鼠PAG区离子钙接头蛋白(Iba)-1阳性细胞数，Western blot法检测各组大鼠PAG区Iba-1、白细胞介素(IL)-1β及P2X7R的蛋白表达水平。结果:与对照组相比，模型组大鼠第2、4、6、8天面部及后足底机械痛阈均显著降低(P<0.01

P<0.001);与模型组相比，电针组大鼠第4、6、8天面部及后足底机械痛阈显著升高(P<0.05

P<0.001

P<0.01)。与对照组相比，模型组大鼠PAG区小胶质细胞显著激活，Iba-1阳性细胞数明显增多(P<0.001)

Iba-1、IL-1β及P2X7R蛋白表达水平均显著升高(P<0.05);与模型组相比，电针组大鼠PAG区Iba-1阳性细胞数减少(P<0.05)

Iba-1、IL-1β及P2X7R蛋白表达水平均显著降低(P<0.05)。结论:电针“风池”能显著改善偏头痛大鼠皮肤异常性疼痛，其可能与抑制PAG区P2X7R介导的小胶质细胞激活相关。

Abstract

Objective To observe the effects of electroacupuncture(EA) at “Fengchi”(GB20) on the ethology

microglia activation and P2 X7 receptor(P2 X7 R) expression in the periaqueductal gray(PAG) in recurrent migraine rat model

so as to explore the underlying mechanism of EA reducing central sensitization of migraine. Methods Thirty-six male SD rats were randomly divided into control

model and EA groups

with 12 rats in each group. Recurrent migraine model was induced using repea-ted dural electrical stimulation once another day(the 1

(st)

3(st)

(rd)

5(rd)

(th)

7(th)

(th)

and 9(th) and 9

(th)

days)

for a total of 5 times; rats in the EA group received EA treatment(2 Hz/15 Hz

0.8—1 mA) at GB20 after dural electrical stimulation

for 10 min every time

once a day for 9 days; rats in the control group only received electrode placement. The facial and hindpaw mechanical withdrawal threshold was detected by using an electronic von-Frey on the 0(th) days)

for a total of 5 times; rats in the EA group received EA treatment(2 Hz/15 Hz

0.8—1 mA) at GB20 after dural electrical stimulation

for 10 min every time

once a day for 9 days; rats in the control group only received electrode placement. The facial and hindpaw mechanical withdrawal threshold was detected by using an electronic von-Frey on the 0

(th)

(baseline)

2(th)(baseline)

(nd)

4(nd)

(th)

6(th)

(th)

and 8(th)

and 8

(th)

days. Microglia activation in the PAG was evaluated by using immunofluorescence staining to detect the number of ionized calcium binding adaptor molecule-1(Iba-1)-labeled microglia. Expression levels of microglia marker Iba-1

inflammatory factor interleukin(IL)-1β and P2 X7 R were detected by Western blot. Results Compared with the control group

the facial and hindpaw mechanical withdrawal threshold of rats were significantly reduced on the 2(th) days. Microglia activation in the PAG was evaluated by using immunofluorescence staining to detect the number of ionized calcium binding adaptor molecule-1(Iba-1)-labeled microglia. Expression levels of microglia marker Iba-1

inflammatory factor interleukin(IL)-1β and P2 X7 R were detected by Western blot. Results Compared with the control group

the facial and hindpaw mechanical withdrawal threshold of rats were significantly reduced on the 2

(nd)

4(nd)

(th)

6(th)

(th)

and 8(th)

and 8

(th)

days(P

0.01

0.001); the microglia in the PAG area were significantly activated

with the number of Iba-1-positive microglia

and the expression levels of Iba-1

IL-1β and P2 X7 R proteins significant increased(P

0.001

0.05) in the model group. Compared with the model group

the facial and hindpaw mechanical withdrawal threshold of rats were significantly increased on the 4(th) days(P

0.01

0.001); the microglia in the PAG area were significantly activated

with the number of Iba-1-positive microglia

and the expression levels of Iba-1

IL-1β and P2 X7 R proteins significant increased(P

0.001

0.05) in the model group. Compared with the model group

the facial and hindpaw mechanical withdrawal threshold of rats were significantly increased on the 4

(th)

6(th)

(th)

and 8(th)

and 8

(th)

days(P

0.05

0.001

0.01)

and the above indicators were significantly reversed(P

0.05) in the EA group. Conclusion EA at GB20 can significantly improve facial and hindpaw mechanical withdrawal threshold of migraine rats

and its possible mechanism may be related to inhibiting microglia activation mediated by P2 X7 R in the PAG.

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