预电针调控瞬时受体电位通道相关蛋白防治荨麻疹的机制研究

李记泉; 李思佳; 王列; 陈怡然; 王鹰; 刘晶晶; 戴俭宇; 王巍; 马铁明

doi:10.13702/j.1000-0607.20220166

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预电针调控瞬时受体电位通道相关蛋白防治荨麻疹的机制研究

机制探讨 | 更新时间：2023-08-11

- 预电针调控瞬时受体电位通道相关蛋白防治荨麻疹的机制研究
- Electroacupuncture preconditioning relieves cutaneous passive anaphylaxis by down-regulating IP3 mediated ROS/TRPM2 signaling pathway and inhibiting mast cell degranulation in rats with urticaria
- 针刺研究 2023年48卷第3期页码：274-280
- 作者机构：
  
  1. 辽宁中医药大学针灸推拿学院
  2. 辽宁中医药大学中医学院
- 作者简介：
- 基金信息：
  
  中国博士后科学基金项目(No.2021M693850);辽宁省自然科学基金博士启动基金(No.2022-BS-199);辽宁省教育厅重点攻关项目(No.L201946);辽宁省“兴辽英才计划”项目(No.XLYC1902004)
- DOI：10.13702/j.1000-0607.20220166
  中图分类号：
- 纸质出版日期：2023
- 稿件说明：
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李记泉, 李思佳, 王列, 等. 预电针调控瞬时受体电位通道相关蛋白防治荨麻疹的机制研究[J]. 针刺研究, 2023,48(3):274-280.

LI Ji-quan, LI Si-jia, WANG Lie, et al. Electroacupuncture preconditioning relieves cutaneous passive anaphylaxis by down-regulating IP3 mediated ROS/TRPM2 signaling pathway and inhibiting mast cell degranulation in rats with urticaria[J]. Acupuncture research, 2023, 48(3): 274-280.
李记泉, 李思佳, 王列, 等. 预电针调控瞬时受体电位通道相关蛋白防治荨麻疹的机制研究[J]. 针刺研究, 2023,48(3):274-280. DOI： 10.13702/j.1000-0607.20220166.

LI Ji-quan, LI Si-jia, WANG Lie, et al. Electroacupuncture preconditioning relieves cutaneous passive anaphylaxis by down-regulating IP3 mediated ROS/TRPM2 signaling pathway and inhibiting mast cell degranulation in rats with urticaria[J]. Acupuncture research, 2023, 48(3): 274-280. DOI： 10.13702/j.1000-0607.20220166.

摘要

目的:观察预电针“曲池”“血海”对荨麻疹大鼠的防治作用，以及对瞬时受体电位(TRP)离子通道相关蛋白三磷酸肌醇(IP3)、活性氧(ROS)、TRPM2、钙调蛋白(CaM)介导肥大细胞(MCs)脱颗粒的影响，探讨其分子机制。方法:SD大鼠随机分为空白对照组、模型对照组、预电针组、阳性药物组，每组8只。造模前10 d

预电针组给予“曲池”“血海”预电针干预，20 min/次，1次/d

连续10 d;阳性药物组每天给予氯雷他定片稀释液灌胃(1 mg/kg)

1次/d

连续10 d。采用卵白蛋白与免疫佐剂稀释液联合注射法制备大鼠抗卵白蛋白血清，皮肤被动过敏反应法制备荨麻疹大鼠模型。计数大鼠背部致敏皮肤搔抓次数，测量大鼠皮肤致敏蓝斑的直径，甲苯胺蓝染色法观察并计数大鼠皮肤MCs脱颗粒率，免疫组织化学法检测大鼠皮肤组织IP3、ROS表达，Western blot法检测大鼠皮肤组织TRPM2、CaM蛋白表达。结果:与空白对照组比较，模型对照组搔抓次数、皮肤致敏蓝斑直径均增加(P<0.01)

MCs脱颗粒率升高(P<0.01)

皮肤组织中IP3、ROS阳性表达及TRPM2、CaM蛋白表达水平均升高(P<0.01)。与模型对照组比较，预电针组、阳性药物组搔抓次数减少、皮肤致敏蓝斑直径减小(P<0.01)

MCs脱颗粒率降低(P<0.01)

皮肤组织中IP3、ROS阳性表达及TRPM2、CaM蛋白表达水平均降低(P<0.05

P<0.01)。结论:预电针“曲池”“血海”可减少荨麻疹模型大鼠背部致敏区域搔抓次数，缩短致敏蓝斑直径，从而抑制MCs脱颗粒，该效应可能与电针调控TRP通道相关蛋白IP3、ROS、TRPM2、CaM表达有关。

Abstract

Objective To observe the effect of electroacupuncture(EA) preconditioning of “Quchi”(LI11) and “Xuehai”(SP10) on mast cell(MC) degranulation

and expressions of inositol triphosphate(IP3)

reactive oxygen species(ROS)

transient receptor potential(TRP) M2

calmodulin(CaM) in rats with urticaria

so as to reveal its molecular mechanism under-lying improving urticaria. Methods Thirty-two male SD rats were randomly divided into blank control

model

preconditioning of EA(Pre-EA) and medication groups(n=8 rats/group). The urticaria model was established by intradermal injection of dilute allogeneic antioalbumin serum at the spots of the bilateral symmetry of the spine on the back

and followed by tail venous injection of mixture solution of egg albumin diluent

plus 0.5% Evans blue and normal saline. Ten days before the end of modeling

rats of the pre-EA group received EA stimulation of LI11 and SP10 for 20 min

once a day for 10 consecutive days

and those of the medication group received gavage of loratadine tablets diluted solution(1 mg/kg) once a day for 10 days. The times of rat's scratching the sensitized skin were recorded

the diameter of the sensitized blue spots was measured and the degranulation rate of skin MCs was counted under microscope after toluidine blue staining. The expression levels of IP3

ROS

TRPM2 and CaM in the skin tissue were measured by immunohistochemistry and western blot

respectively. Results Compared with the blank control group

the scratching times

diameter of the sensitized blue spots

degranulation rate of MCs

and the expression levels of ion channel related proteins(IP3

ROS

TRPM2 and CaM) were significantly increased(P<0.01) in the model group. In comparison with the model group

the scratching times

diameter of sensitized blue spot

degranulation rate of MCs

and the expression levels of IP3

ROS

TRPM2 and CaM in both pre-EA and medication groups were significantly down-regulated(P<0.01

P<0.05). No significant differences were found between Pre-EA and medication groups in down-regulating the levels of the above-mentioned 7 indexes. Conclusion EA-LI11 and SP10 preconditioning can reduce the cutaneous anaphylaxis in urticaria rats

which may be related to its effects in inhibiting the degranulation of MCs

and the expression of TRP channel related proteins.

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