摘要:五年来(1975~1979) 的临床实践和实验研究证明,按胃大部切除手术过程中经常出现的症状辨证循经选穴,能够充分发挥针刺的调整作用,从而促使人体内发生一系例的规律性变化,并明显地提高了胃大部切除术的针麻效果。为了深入研究体内这些变化的发生机理,需要有一个近似针麻手术病人变化的实验动物模型。为此研制了本实验模型.Subtotal gastrectomy was performed on 107 dogs,93 of which were operated under acupunctureanesthesia.The needling points were Xinshu,Neiguan,Pishu,Zusanli.The acupuncture anesthetic ef-fect was graded as:Excellent(Ⅰ),good(Ⅱ),poor(Ⅲ)and non(Ⅳ).In these 93 dogs,theanesthetic effect was excellent in 50(54%),good in 27(29%)and poor in 16(17%).During incision of the skin and peritonium,clamping gastric arteries(after 0. 5% procain 0. 2-0. 5ml infiltration),retracting the stomach,clamping and cutting the duodenum and the stomach,aswell as during gastro-intestinal anastomosis,there was no response in grade Ⅰ;in grade Ⅱ,the respon-ses to pain and visceral tractional sensation were slight and short;in grade Ⅲ there were marked andprolonged responses.On 12 dogs subtotal gastrectomy was performed without acupuncture but only with supplementarydrugs.All of these dogs struggled and barked during the whole operation.As for the dogs operated with neither acupuncture nor supplementary drags,the operation wasvery difficult to perform.At the end of the operation traumatic shock occured.This exprimental model not only can be used to prove the anesthetic effect of acupuncture butcan also be used to further the study of the mechanism of acupuncture anesthesia.
摘要:已有许多文献报导血浆多巴胺-β-羟化酶(DβH)活性可以作为交感神经活动的指标,过去,我们曾用血浆DβH 活性来反映交感神经活动,在针麻胃大部切除术和子宫切除病人中观察到:在针麻效果好时,诱导后和术中血浆DβH 活性下降或不变,而针麻效果差者,血浆DβH 活性上升,Plasma DβH activity of dog during subtotal gastrectomy under acupuncture anesthesia was deter-mined by the spectrophotometric method.It was found that the change of DβH activity was closelyrelated to the acupuncture effect.In dogs with excellent acupuncture anesthetic effect after induction and during operation the pla-sma DβH activity decreased.Whereas when acupuncture anesthetic effect was poor, plasma DβH acti-vity increased continuously after induction and during operation.These results are similar to thoseof patients during subtotal gastrectomy under acupuncture anesthesia.
摘要:我们曾观察到针麻手术病人术中网膜和胃壁交感神经介质荧光组织化学变化及胃壁神经的超微结构变化与针麻效果有关。针麻效果较好者,其交感神经纤维及末梢内含有较多的去甲肾上腺素,针麻效果差者,含量明显减少。动物在针麻下,其变化又是怎样?为了进一步验证这一变化规律,我The changes of noradrenaline content in the adrenergic nerves of the stomach wall have beeninvestigated by glyoxylic acid-induced fluorohistochemical method.The experimental results revealed that there were more noradrenergic nerve terminals with morefluorescent intensity in the dogs with excellent effect of acupuncture anesthesia,but less noradrenergicterminals with weak fluorescent intensity in those with poor effect of acupuncture anesthesia.These results suggest that there is an increase of storage and a decreased release of noradrenalineduring operation in dogs with good acupuncture anesthetic effects;on the contrary,an increased re-lease with decreased storage in those with poor effects.All these changes are similar to those observedin clincal patients.
摘要:5-羟色胺(5-HT),是体内一种具有广泛生理功能的活性物质。中枢神经内的5-HT 参与针刺镇痛,已有不少报导。但在外周5-HT 则是受人重视的一种具有强烈致痛作用的物质,针刺对外周5-HT 作用的研究尚少。为此,我们曾In previous clinical studies,we found that in patients with excellent acupuncture anesthetic effectplasma free serotonin (5-HT) level during operation was lower than in patients with poor effects.Inthe present study the changes of 5-HT content in the whole blood of 53 dogs during subtotal gastrec-tomy under acupuncture anesthesia have been determined by spectrophotofluorometric assay.The results showed that the increase rate of 5-HT in animals with poor anesthetic effects during operation washigher than that in those with excellent anesthetic effects.This result may be related to the increasedstorage and decreased release of 5-HT in APUD cells as well as in platelets.It has been suggested thatthe above changes reflect one aspect of the acupuncture regulatory functions.
摘要:前言在针灸,针麻原理的研究中,体表皮肤电阻(确切地说是阻抗)的测定是一个重要的手段。由于人体属于离子导电,皮肤电阻的大小与细胞膜的离子通透性直接有关,也与测量时,所施的电流性质有关。用直流测量时,由于组织的极化现象,阻值与电流的极性、强弱和时间有关,是一个变In the research of acupuncture treatment and acupuncture anesthesia,measuring the impedance ofthe skin is an important approach.In order to maintain the original state of the skin and to prevent distortion caused by measuringcurrent,a low level A.C.current skin impedance meter was used.Two different kinds of high freque-ncy and low frequency instruments are available,for measnring impedance of different zones.The feature,working principle,making and operation of these two instruments are introduced inthis report.
摘要:目前针刺麻醉的临床实践和理论研究工作中,普遍采用脉冲电波--针麻仪来代替手法运针,作为针麻中维持穴位针感的刺激方法。临床实践中,人们普遍地认为电刺激所取参数与针麻效果关系密切,近年来,一些单位专门对电针刺激参数进行了研究,也发现电刺激参数在一定程度上影响针麻效In the paper,through a test for the output impulse parameters of acupuncture-anesthetic apparatusunder stimulating loading and during operations under acupuncture anesthesia,the problem present inthe measurement of the electric stimulation of the acupuncture-anesthetic apparatus type G-6805 widelyused at present is discussed.It is suggested that taking the current value as a parameter indicator for theintensity of stimulaiton is reasonable,and a way of solving this problem is presented.
摘要:在针麻动物模型的实验中,需要比较精确的测定电针的刺激参数,以便掌握不同的刺激参数对针麻效果的影响。但是一般针麻仪的刻度都不够精确,而且刺激的脉冲电流也随机体阻抗的大小而变化,因此很难从针麻仪刻度盘上读出精确的刺激参数值。由于针麻仪输出的电流脉冲都很窄(约在0. 3毫For the precise measurement of parameters during acupuncture anesthesia,a sampling resistor isconnected into the output circuit of model 57-6D electroacupuncture apparatus.The current intensity and frequency of electro-acupuncture for stimulation through the organismcan be accurately measured by monitoring the voltage across the sampling resistor.
摘要:在针刺镇痛原理和痛觉生理的实验性研究中,大白鼠是被研究和应用得较多的一种动物,有人采取闭式装置,即将动物装入硬质筒内,这样只能使其身体的一部分露于筒外。我们是采用敞式法,将清醒大白鼠比较自然的固定在特制的实验基座上,使其身体的绝大部分敞露于外。这对选择伤害性An apparatus of exposed type for restraining the rat in acupuncture analgesia research has beendesigned.During the experiment the rat was restrained on a wooden base having a shallow throughwith two canvas-bands passing over the posterior of the animal's shoulders and the anterior of thecoxae respectively.In contrast with the commonly used hard tube,the apparatus has the followingadvantages:Most parts of the body surface are exposed,the animal is being kept in natural position,and the respiration remains normal.The restrain is not causing uneasiness,consternation,struggle,gnawing,attack etc.gnawing 260 experiments were performed on 48 rats.A single square wave impulse delivered from theC 64-2 multi-wave therapeutic stimulator was applied to the tail skin of the unanesthetized animals.Thepain response was indicated by tail flick and screaming of the animals.The pain threshold was mea-sured in voltage of stimulation.Acupuncture analgesia was then induced by electricali needling at cer-tain points corresponding to the“Renzhong”point,“Jiaji”points and bilateral“Zusauli”points inman respectively with the repetitive wave impulses which were delivered from the G-6805 therapeuticstimulator.(Continued on p.232 The results of the experiments indicated that after analgesia being induced with electrical needlingat above-mentioned points separately,the mean values of pain threshold increased significantly ascompared with those before electrical needling and of control group.On the other hand,there is nosignificant difference in the effect of acupuncture analgesia among these three poin-groups.The sameexperiments were repeated after an interval of one and half months,the pain threshold values and theeffect of acupuncture analgesia were shown to be relatively stable.
摘要:巴多胺-β-羟化酶(DβH)可促使多巴胺-β-位羟基化为去甲肾上腺素,不少学者提出DβH 活性可以作为交感神经功能活动的指标、但也有人持有不同的看法。我们自1975年以来测定针麻病人血浆中DβH以及针麻狗模型血浆及脑组织中DβH 的活性,企图探讨针刺对交感神经系统调整功In this paper an improved spectrophotometric assay for dopamine-β-hydroxylase is described.Themethod is simpler and easier to perform than enzymatic radioassay.The optimal conditions for the use of this method is discussed.
摘要:本文介绍了近年来国内外运用计算机累加技术,从人体头皮上引出的累加脑诱发电位作为疼痛客观测量方法的研究及其动态,脑诱发电位中某些早反应(潜伏期30毫秒左右)和晚反应(潜伏期160~260毫秒)的波形成份和疼痛关系密切.人脑诱发反应研究的进展为实验性疼痛的估价提供了新的方向。但还存在一些问题.In this paper a objective method of pain measurement with the cerebral evoked potential from scalpby computer average technique and the development in this field is presented.The components of earlyresponse (latency adout 3 ms) and late response (latency about 160-260 ms) in the cerebral evokedpotential were closely related to pain perception.In this review,it has pointed out the way for theevaluation of exprimental pain but some questions are still exist.