摘要:十年来,循经感传现象的研究在全国各地广泛开展,取得了显著的成绩。本文的目的不是对已经发表的大量资料进行概述,而只是着重考虑近年来感传研究所取得的一些主要成就和当前工作中存在的问题,并对有关感传形成机制的两种观点以及感传研究的意义进行了扼要的讨论,以期对今后的工作有所助益.Studies on the phenomenon of the propagated sensation along channels
(PSC)have been conducted extensively in our country for ten years and
a remarkable success had been achieved.This review does not attempt to
cover the enormous literatures accumulated in that time.Instead,attention
will be concentrated on the main results had been achieved and some difficult
problems to be solved at present.Lastly,the different points of view con-
cerning the mechanism of the formation of PSC and the physiological
significance of PSC research are briefly discussed.It is hoped that this
review may be somewhat beneficial to the further investigations from now
on.
摘要:临床实践证明,腹部针麻手术效果的好坏,常常受内脏牵拉反应和内脏痛的影响.因此,如何寻找能够预测内脏牵拉反应的方法,是选择针麻适应症,提高针麻效果的重要因素之一.方法病种分腹部及颈面部手术两组。腹部有全子宫切除术、胃大部切除术;颈面部有甲状腺囊肿或腺瘤切除术,青光眼减压术共Clinical practice suggests that the effect of acupuncture anesthesia is
unstable and has close relation to individual conditions,esp.in abdominal
operations such as gastrectomy,hysterectomy,cesarean section,oophoro-
cystectomy,etc.In these procedures,refractive reaction and pain of the
abdominal organs are more remarkable.In order to find out an appropriate
individual for the acupuncture anesthetic operations,the alteration of heart
rate before and after needling Neiguan point as the presumptive index for
the acupuncture anesthesia is presented as a good method in this study.
The heart rate should first be recorded when the patient is in a rest
state.After that,needling Neiguan point and an electric inducement should
be continued for 20 minutes.Then,the heart rate should be recorded again.
I88 patients were tested in this study and the alteration of the heart rate
was compared with the postoperative acuupuncture anesthesia effect.It was
found that the slowed-down heart rate produced after needling Neiguan point
was in a direct proportion to the effect by acupuncture anesthesia on the
abdomen,i.e.those had a slowed-down heart rate would have a good effect,
while those had an unaltered or increased heart rate would have as unde-
sirable effect.The corvespondent rate is 77.5%.
摘要:针刺麻醉术前预测对了解个体差异与针麻效果的临床规律、寻找提高针麻有效方法、选择针麻适应症和阐明针麻原理均具有重要意义.目前采用预测指标繁多、方法各异,且易受主观成份及客观因素影响,因而结论往往不一,所以在条件相对稳定,方法相对统一的情况下研究一些方法简易、符合率高,受主、客观因素干扰小的预测指标仍是当前The preoperative estimation of acupuncture anesthesia effectiveness is
of great importance in evaluating the relationship between individual varia-
tion and acupuncture anesthesia effect.This paper introduces a study on
various preoperative estimation methods,including the indices of touch
threshold measurement and pulse rate.
The pulse rate of the patient undergoing gastrectomy was measured
with an electronic pulse rate instrument during three to five days before
operation.
The changes in pulse rate were taken down seperately when patients
lying down,after twirling of acupuncture needle for 15 min and again 10
min.after the removal of the acupuncture needle.Changes of the touch
threshold were measured on the intended part of incision with a BY-7A touch
threshold data reading equipment.By double blind method,we compared
the average values of the changes with the acupuncture anesthesia effect.
The points used for surgery as well as the method of acupuncture,para-
meter of stimuli,were all the same,the explanations for measures taken
were played off a tape recorder. 1.The relationship between the change in pulse rate after acupunctuer
and the effect of acupuncture anesthesia.
The average decrease of the pulse rate after acupuncture in grade 1-2
was 4.5 beats/min.(P<0.01)and in grade 3-4 was 1.3 beats/min.(P>0.05).
The effect on pulse rate persisted 10 min.after the acupuncture needles
were removed.The change of pulse rate in grade 1-2 was more obvious
than in grade 3-4 from beginning to end.
After insertion of the needles,the change of the pulse rate may be
classified into three classes,i.e.increase,decrease and unchanged.These
were compared seperately with the actual effect of acupuncture anesthesia,
the total rate of correspondence being 74.3%.The rate of correspondence
in grade 1-2 was 92.3%.
2.The relationship between the change of touch threshold and the
effect of acupuncture anesthesia:
After the insertion of the needles,the average value of touch thre-
shold in grade 1-2 changed from 10.05(mA)to 10.36(mA)(P>0.5).
The average value of touch threshold after needling in grade 3-4 decre-
ased from 9.81(mA)to 8.32(mA)(P<0.01).
The preoperative predict of the effect of acupuncture according to chan-
ges of the touch threshold was carried out.The total rate of correspon-
dence was 92.1%.Moreover,the test of the touch threshold can be re-
peated with unchanged results.
The investigation shows that both the pulse rate and the touch thre-
shold measurements can be used as indices for preoperative estimation,
the touch threshold being more stable and reliable with a high rate of
correspondence.
It was found that the changes of the touch threshold and the pulse
rate after needling were more marked in grade 1-2 than in grade 3-4.It
is suggested that the relative specificity of points and the individual pecu-
liarity of grade 3-4 patients be further investigated.
摘要:针刺镇痛和对抗手术中生理扰乱的调整作用,已被国内外针麻临床和实验研究所确认.但针刺效应可持续多久?尤其是针刺的后效应对机体康复起多大作用?没有引起应有的注意。深入研究针刺的后效应,将有助于全面地认识针刺对机体的作用和针刺的基本原理的阐明。本文选择溃疡病胃大部切除术患者103例,观察术后康复过程中创伤性“手术热”的程度与持续时间、血液白细胞变化、术后伤口痛时腹直肌肌电图的变化和肠蠕动功能恢复时间等指标,了解到针刺的后效应主要表现为抗手术创伤和促进机体康复两方面的作用,现将研究结果报告如下:The analgesic effect of acupuncture and its adjusting effect on physio-
logical disturbances of the surgery have been proved at home and abroad
by clinical and experimental research.
This paper reports 103 selected cases of partial gastrectomy in peptic
ulcer patients,comparing the data of phyological and biochemical changes
during the postoperative recovery;the study of post-acupuncture effects
in cases having undergone acupuncture anesthesia,epidural anesthesia and
general anesthesia,resulted in the following findinds:
A.The post-acupuncture effects on“post-operative fever”.
The average rise in temperature caused by surgical trauma(“post-opera-
tire fever”)usually does not exceed 38.2℃.In observing continually for
a week after operation,the“postoperative fever”of the acupuncture group
is lowr than that of other groups.The average duration of“post-operative
fever”of the acupuncture anesthesia group is 3.45 days,of the epidural
anesthesia group,4.91 days,and of the general anesthesia group,6.9 days.
The difference is very significant.(P<0.001).
The post-operative change of the pulse rate in the acupuncture anesthesia
group was less than that in other groups,with a marked difference on the
first day after operation compared with the epidural anesthesia group(P<
0.05).This may indicate that the resistance of the body against surgical
trauma has been increased after acupuncture.
B.Post-acupunture effect on against infection:
In comparing the total white cell and neutrocyte count on the second
day after surgery with those before it,both of these counts were increased
in all three groups;however the increase in the acupuncture anesthesia
group was most significant(P<0.01).The total white cell count increased 70、2%,the neutroeytes increased 23.4%.As professor Гордиенко pointed
out,the increase in wbc is proportional to the ability of phagocytosis
According to a report from Beijing,acupuncture on Zusanli point will
increase the ability of phagocytosis lasting for 72 hours.This statement
is in coincidence with our experience,indicating the existance of a post-
operative antiinflammatory effect.
C.The postoperative analgesic effect on the incisional wound:
The electro-myogram of the rectus muscle was observed continually
for 3 hrs after operation.The average accumulated voltage of electromyo-
dium in acupuncture anesthesia and epidural anesthesia were 14.2 μV and
13.7μV.respectively,the difference being insignificant.However,it was
found that during the first 1.5 hrs.the microvoltage in acupuncture anes-
thesia cases was higher than that of the epidural anesthesia cases,while
in the later 1.5 hrs.in acupuncture anesthesia,it was lower than that in
the epidural anesthesia.This shows that the efficacy of acupuncture anls-
thesia was not so good as that of the epidural anesthesia.But after removal
of theneedles,the analgesic effect against postoperative incisional pain did
persist to a certain extent.
D.The post-acupuncture effect on recovery.
In observing the duration needed for the first flatus expulsion after
operation,the average duration in the acupuncture anesthesia group was
44.15 hrs,and in the epidural anesthesia group 56.12 hrs.This shows that
aeupuncuure can mitigate physiological disturbance of the intestinal func-
tion,and be beneficial to recovery.
摘要:针灸治疗猴细菌性痢疾的疗效和部分机理的讨论已在前文中报导。为了对针灸治疗细菌性痢疾过程中与痛觉有密切联系的胆碱能神经末梢形成和释放进行观察,我们系统分析了针灸治疗前后和整个治疗过程全血胆碱酯酶活力的变化,同时还对与免疫功能有关的血清蛋白质在针灸过程中比例的变化进行了醋酸纤维薄膜电泳的分析,并且拍摄了聚丙烯酰胺凝胶盘状电泳(disc 电泳)的图谱,对血清清蛋白、α-球蛋白、β-球蛋白和γ-球蛋白中 IgA、IgM、IgG 电泳行为进行了讨论.现将结果初报如下:This paper reported the results of whole blood choline esterase,serum
protein ratio and polyacrylamide gel electrophoresis(Disc)that were obser-
ved in the course of monkeys' experimental bacillous dysentery treated by
acupuncture and moxibustion.When the monkeys were infected and suffered
from dysentery,the activity of blood choline esterse decreased 17.43%(the
maximal decreasing value was 25.98),which denoted the pain causing ma-
terial acetyl choline began to accumulate when the monkeys were sick.After
acupuncture treatment,the pain of the sick monkeys was alliviated or di-
sappeared,then the actvity of blood choline esterase returned to normal
level gradually.
The mechanism of acupuncture and moxibustion treatment is to improve
the effectiveness of immumne system in sick monkeys.In order to identify
this situation,we have tested the serum protein ratio by cellulose acetate
membrane electrophoresis,and the results presented that when the monkeys
were infected by experimental bacillous dysentery,then the r-globulin in
their serum decreased from normal level 18.08% to 12.32%.After acupun-
cture treatment the quantity returned to the normal level two months later.
It means that mechanism of acupuncture and moxibustion may improve
the effectiveness of immune system in the monkeys.
摘要:目前针刺麻醉的临床实践和理论研究工作中,普遍采用针麻仪代替手法运针,作为维持穴位针感的刺激方法。临床实践中人们普遍地认为选用的电针刺激参数与针麻效果关系密切.因而合理地掌握刺激量是提高针麻效果的一个重要条件。这就要求我们对电针的刺激参数进行筛选,以求得针麻适宜的刺激条件.为此目的我们在北京大学高等数学教研组和生物系的指导下设计了如下一组动物实验来观察针麻仪(电针)主要的几种刺激参数对针麻效果的影响.From the neurophysiologic point of view,the stimulating effects of ele-
ctric needle in acupuncture anesthesia is a kind of induced effect causing
transmission of nerve impulse.In the instrument design,it is not necessary
to provide stronger power output as for electric therapy or electric an-
esthesia.Since 1966,we have successfully applied the intermittent oscillator
as the main oscillating circuit in the operations under acupuncture anesth-
esia.For more than 10 years,the fundamental circuit design was mostly used
in various types of acupuncture anesthesia apparatus.
Through the clinical and experimental observations,it was found that
there were 4 characteristics in the pulse produced:
1.High pulse amplitude,usually reaching 1-200 V(peak-to-peak pulse
amplitude).
2.Small pulse,width range within 0.2-0.5ms.
3.Accumulating volume of DC component is small,or even negligible,
so that there are no burns and electrolysis produced.
4.High ouput impedance.
In order to observe the relationship between the stimulating parameters
of electric needle and the effects of acupuncture anesthesia,and to further
improve the stimulating parameters of electric needle,we simulated the acute
trauma of surgical abdominal exploration as the pain stimulation,and took
the stuggle reactions in experimental celiotomy of animal models as the pain
index,through the quantitative calculation of the pain struggle reactions
of the rabbit during the course of operation under acupuncture anesthesia.
We screened the parameters of the strength,wave width,duty ratio and
frequency of electric needle.The results obtained were follows.
1.The main factor influencing the effects of acupuncture anesthesia in
the strength,wave width,duty ratio and frequency is the strength of stimula- tion.
2.The best stimulating strength chosen in this experiment is identical
with the standard of stimulations selected in the clinical operations under
acupuncture anesthesia,e.g.the largest stimulation strength which could be
tolerated by the patient.
3.The relationship between the variation of the frequency within 10-100
kHz and the effects of acupuncture anesthesia was not correlated markedly,
Thus,the determining factors of frequency,e.g.,the combination of wave
width and duty ratio should be further studied in the future.
摘要:对于心理因素在针刺麻醉中的作用,人们的看法殊不一致。有关情绪状态,暗示等心理因素对针麻效果的关系,已有过一些探讨。在我国针刺麻醉的大量临床实践中,多数单位在术前对病人均作“解说”工作,主要内容包括介绍针麻的优越性及尚存在的缺点,术中主要步骤有何感觉,以及应如何配合等。病人的这种“思想准备”对针麻效果有何意义,是否必要,这是一个应The purpose of thie study was to explore the influence of preopera-
tive explanation on the effect of acupuncture anaesthesia.48 normal subj-
ects were divided into readiness and non-readiness groups according to
preexperimental instructions and the presence or absense of advanced in-
formation of the stimulation applied.The two groups were matched accor-
ding to sex,age,as well as their sensitivity of pain and acupuncture.
The results were as follows:
1.Pain feelings and G.S.R.responses to stimuli of the non-readiness
group were greater than those of the other group,especially in sensitive
subjects.The evoked responses of pulse amplitude and the compounded
pulse,respiration and G.S.R.responses were different between the two
groups only in the sensitive subjects,showing that the sensitive subjects
in the non-readiness group responded more strongly.
2.Both the pain feelings and the compounded evoked physiological re-
sponses of the sensitive subjects were significantly higher than those of
the non-sensitives in both groups.
3.The results show that knowledge or mental readiness of acupunc-
ture analgesia,i.e.the time,quality,feeling of stimuli,played only a
moderate role in acupuncture analgesia.It seemed that prior-explanation
of acupuncture had some significance,but its effect was limited and varied
with subjects.
摘要:近十余年来,对于环-磷酸鸟苷(cGMP)在中枢神经系统中的机能作用引起了人们的注意.有报导 cGMP 具有镇痛性质,并假设 cGMP 或许是一种或一种以上的神经递质的调节者。本工作是观察脑室注射cGMP 对针刺镇痛的影响,并探索它与乙酰胆碱及阿片样物质之间可能存在的关系.The purpose of this study is to observe the effects of intraventricular
injection(IVT)of cGMP on acupuncture analgesia(AA)and its relationship
with acetylcholine and endogenous opiate like substances.The following
results have been obtained.
1.After the intraventricular injection of cGMP(400μg)pain thresho-
lds of rats were markedly elevated (47%~63%).During continuous electro-
acupuncture for thirty minutes,the average pain threshold increased by
47%.Intraventicular injection of cGMP associated with additional electro-
acupuncture elevated markedly Pain thresholds(130%).The effect was
more that of arithmetic sum of cGMP plus electro-acupuncture.These
results indicated that cGMP enhanced the effects of AA.
2.The effects of AA decreased by 18% at 3 hours after IVT of hemi-
cholinium-3(50μg).On the basis of that,cGMP raised the effects of AA by
42%,but the effect failed to reach the original level.The result suggested
that the enhancement of cGMP on AA could be partially antagonized by
hemicholinium-3.
3.The effect of AA of rats could not be blocked by the IVT of
naloxone(30μg).When cGMP was used in combination with naloxone,
the reenforce effect of cGMP on AA was not shown.The results suggested
that the enhancement action of cGMP on AA could be antagonized by bloc-
king the opiate receptor with naloxone.
摘要:在针刺镇痛的作用中,不少资料证明与中枢神经化学介质有一定关系。究竟那些神经介质,它们各自起什么作用,这是一个极为复杂的过程。我们只是企图从中枢神经系统不同区域内乙酰胆碱(ACh)的变化来探讨。一般的组织化学方法至今还不能直接显示乙酰胆碱。但脑内有些区域乙酰胆碱(ACh)、乙酰胆碱酯酶(AChE)、胆碱乙酰化酶(ChAT)的含量多少与活性的高低大体是一致的。所以我们在电针刺激的条From the point of view that AChE and ChE are related to neurotra-
nsmitters,the histochemical changes of these enzymes in different areas of
the CNS of rat were taken as the experimental index to investigate the
mechanism of acupuncture analgesia.
223 rats(male 115,female 108)weighing 115 to 220 grams were divided
into 3 groups:Group 1,SGS studies on subtantia gelatinosa of spinal cord.
Group 2,TST on nucleus tra(?)tus spinalis n.trigemini and group 3,LC on
locus caeruleus.Each group was divided into three subgroups.A pain-sti-
mulation,a pain-stimulation plus acupuncture and a control subgroup.
Basic pain thresholds were measured.The loci of pain-application were as
follows:In the group SGS,at the right third intercostal skin,in group
TST,at the left upper lip,and in group LC,a“mimetic abdominal opera-
tion”was performed as in pain-stimulation.Different points used for
acupuncture were Zusanli Neiguan;Hegu and Neiguan,Zusanli,Shangjuxu
and Huatuo Jiagi“Qiekoupang”respectively.At the same time,electromyo-
graphic changes in the M.rectus abdominis were observed as an additional
experimental index.
The Karnovsky-Root's method with some modifications according to
EL-Badawi-Schenk's technique was used for demonstration of AChE and
ChE.
The experimental results showed that some regularities seemed to exsist
concerning the changes of activity of AChE and ChE under different con-
ditions of acupuncture analgesia.There was an obvious decrease of AChE
and ChE activity in the substantia gelatinosa as well as the nucleustractus
spinal n.trigemini.However,ACHE activity in locus caeruleus increased
after electroacupuncture.
Pain stimulation alone resuted in just the con Contrary trary to the
above data(P<0.01).
The evidence mentioned above suggests that the AChE activity in dif-
ferent areas of the rat brain may respond to the process of acupuncture and
the corresponding cholinergic neurones in CNS might playa certain role in
the analgesic and regulatory effects of acupuncture anesthesia.
摘要:文献记载,杏仁核不仅同下丘脑,中脑网状结构,中央灰质,尾核头部有密切的纤维联系,也与内侧丘脑,后丘脑有一定的纤维联系.关于杏仁核同疼痛的关系,国内外已有一些报导.如:杏仁核含有丰富的内啡胀和吗啡受体,向杏仁核内微量注射吗啡和5-羟色胺可以提高动物的痛阈,抑制动物的攻击性行为,电刺激杏仁核可以提高动物的痛阈.可见,杏仁核同痛反应有一定的关系.为了探讨杏仁核及The role of amygdala in acupuncture analgesia was studied in rabbits
with chronically implanted electrodes in the amygdala.Rabbits weighing
1.5-2.Skilogams were used for this study.By means of the stereotaxical
apparatus two pairs of electrodes were implanted bilaterally into the amygd-
ala accotding to Sawyer's atlas.Three days after operation all experiments
were carried out.Iontophoresis of potassium ion to the skin of rabbit's ear
served as the noxious stimulation.Minimal electric current(mA)required
to produce the defence reaction of the head and forelimb was taken as the
pain threshold.Control pain threshold was determined before experiment.
The parameters of electric stimulation were 150-250mA,5-10Hz,0.5mSec.
At first,amygdala was stimulated unilaterally during one minute,and then
noxious stimulation was given.Both stimulations were stopped at once when
the reaction of the pain presented.
In the 14 rabbits an increase of 25.55%(P<0.01)in the pain threshold
occured when the amygdala was stimulated unilaterally.The control group had no effect on the pain threshold(P>0.05).
Whe pain threshold had risen strikingly during amygdala stimulation,
naloxone(0.4mg/kg)was administre.The result was that the raised pain
threshold fell in 13 rabbits,showing reversal of analgesic effect in various
degrees.In the control experiment,7 rabbits were injected with normal
aqueous solution.No noticeable change of the analgesic effect was found.
The difference of two experiments was statistically significant(P<0.05).
The result suggest that the raised pain threshold can be reversed by nalox-
one.
In 11 rabbits,there was,befor lession of the bilateral amygdala,art
increase of 41.0%(P<0.01)in the pain threshold following electro-acu-
puncture.After lession only an increase of 25.75% was produced by electro-
acupuncture.The difference was statistically significant(P<0.05),sugges-
ting a marked decrease of the effect of acupuncture analgesia after the am-
ygdala was damaged bilaterally.
摘要:按照祖国医学理论,针刺作为外因,通过体内调节系统实现各种治疗作用,针刺镇痛则是通过加强和激活体内痛与抗痛调节系统而凑效.以往工作纳洛酮能大部分阻断针刺抑制内脏痛反应,说明内源性吗啡样物质包括在体内抗痛系统内而参与针刺镇痛作用.国内外研究吗啡镇痛作用时,发现室周一导水管周围灰质(PAG)是吗啡的高效区,电刺激脑干某些区,特别是中脑Recent experiments have demonstrated that serotonin,as a neuro-transmit-
ter,is involved in acupuncture analgesia and that NRM plays an important
role in the descending inhibitory control on nociceptive information,In our
present study the role of NRM in acupuncture anti-visceralgia was investi-
gated.
Experiments were performed on male,awake and restrained rats.Elec- trolytie lesions(0.8=1.0mA, 8-10 sec.)of NRM were made using a 0.5mm
exposed tip electrode inserted stereotaxically into NRM 5-7 days before
experimentation.Analgesia testing was carried out with splanchnic nocicep-
tire defensive reflex measured by induced abdominal muscle discharge.
It was found that antivisceralgia produced by electro-acupuncture(EA)
was markedly reduced in the lesion rats whereas the effects of EA was still
significant in sham lesion rats.
In addition,NRM central stimulation delivered through an implanted
concentric electrode with 100μA constant current could produce similar anti-
visceralgia effects as EA.
These results show that NRM activated by EA plays an important role
in producing antivisceralgia.The possible correlation between the endogenous
opiate systme and the serotoninergic system involved in acupuncture analge-
sia was discussed.
摘要:内脏的感觉是由植物性神经传入,早在Langley 及 Pickinson 时已有研究,并被神经学者所公认。Ranson 著《神经系统解剖学》中谈到,“1914年 Chase 和 Ranson、1918年Ranson 和 Billingsley 等认为内脏传入纤维起源于脑脊神经节细胞,它们的纤维经过相应的脑脊神经、植物性神经及神经节,直接到达内脏。这些纤维粗细不等,包含粗细有髓维和纤许多无髓纤维”。Carpenter 也认Twenty seven rabbits and fifteen eats were used.To each animal 80-100μl
of 10%horseradish peroxidase(HRP,Sigma VT)solution was injected into the
tunica serosa of the stomach(anterior wall)of each animal.After a survial time
of five days,the animals were sacrificed and their hearts were perfused
with 2% paraformaldehyde solubed in 0.1M phosphate acid buffer(pH7.2-7.3).
The spinal ganglia were treated according to HRP method and incubated in
3. 3-diominobenzidine(DAB)H_2O_2 solution.The distribution of the HRP labeled cells were examined under microscope and the results were as folllows:
We could find clearly labeled cells appearing in the spinal ganglia from
cervical 4(C4)to lumbars,ofwhich ganglia THS-9 were the segments where
the labeled cells were the most numerous,cervical ganglia 4-8(C4-8)next,and
thelumbus labeled cells were themost minimum.Moreover,it was found that
the labeled cells of different sizes contained large cells and small cells,
but the labeled cells were mainly small cells,and the large cells were
comparatively less.
A new point of view has been put forward by the above experimental
studies.It suggested a new idea,i.e,the segment of the sympathetic
afferent fibers of the stomach is longer(from cervical 4 to lumbus).
摘要:寻找一个较为理想的针刺镇痛模型是深入研究针刺镇痛原理的重要前提,为此我们对钾离子透入甩尾测痛法及其在针刺镇痛模型上的应用作了一些探索.实验方法【测痛电极的制作】取两条胶布(1×3.5厘米),剪一窄而长锡薄纸(宽约0.4厘米)固定在胶布上,胶布两端露出锡纸约0.5厘米,以备与导线上的夹子相连接。然后把分别浸泡有饱和氯化钾溶液及生理盐水的棉花条放在锡纸中间,此电极即可备用。实验前用酒精棉球擦净鼠尾,A model of acupuncture analgesia in rats was established.On this
model the potassium iontophoresis tail-flick method for measuring pain
was used.
The stimulating electrode was very simple and convenient.It was made
of two narrow pieces of silver paper on which a little piece of cotton sep-
aratly saturated with KCI(saturated sulution)or NaCl(0.9%)was put.
In experiments the electrodes should be tightly fixed on the tail of rat with a strip of adhesive plaster.
The apparatus for measuring pain has a negative feedback circuit.So
the stimulating current may increase smoothly and is not influenced by any
changes from the organism impedence.
Electro-acupuncture was given bilaterally at“pishu”with 60 cps.1-2
mA;“zusanli”with 20 cps,2-4 mA.
168 out of 195 rats weighing 150-250g had a comparatively stable pain
threshold within one hour or even longer.The range of fluctuation of
the pain threshold was+7.1~-7.8%.
After electro-acupuncture induction for 10-15 minuts,the pain thres-
hold increased from 0.64±0.03 mA to 1.44±0.05 mA in 35 out of 45 rats.
The difference between the values before and after aupuncture was statist-
ically significant(P<0.001).But the pain threshold in control rats rem-
ained unchanged during the whole process of the experiment.
This model has been used for studying the mechanism of acupuncture
analgesia.